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  2. pSKI

    Plasmid
    #232484
    Purpose
    pSKI can be used for single-copy transgene expression within the Universal SKI System or for extrachromosomal arrays. It contains an MCS with 47 single cuts and two synthetic 900 bp homology arms.
    Depositor
    Carlos Silva García
    Article
    Dinneen et al bioRxiv [Preprint]. 2024 Dec 10:2024.12.
    Insert
    pSKI
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    232484-plasmid-map-sequence-id-464988

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  10. pRegev_ExonSkip_Backbone

    Plasmid
    #204187
    Purpose
    Backbone for cloning splicing library with truncated CAG promoter and first half of beta globin minigene reporter (exon 1 and intron 1).
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    First exon and intron for beta globin splicing minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Truncated CAG
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    204187-plasmid-map-sequence-id-404040

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  16. SKILA

    Plasmid
    #42418
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SKILA (SKIL Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. pBluescriptSKII+ U6-sgRNA(F+E) empty

    Plasmid
    #74707
    Purpose
    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence
    Depositor
    Eugene Yeo
    Article
    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30
    Insert
    U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    74707-plasmid-map-sequence-id-152737

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  18. pBluescriptSKII+ U6-sgRNA(F+E) ACTB

    Plasmid
    #74705
    Purpose
    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of ACTB mRNA
    Depositor
    Eugene Yeo
    Article
    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30
    Insert
    U6 promoter driving sgRNA targeting the 3'UTR of ACTB mRNA
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    74705-plasmid-map-sequence-id-151422

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  19. pBluescriptSKII+ U6-sgRNA(F+E) TFRC

    Plasmid
    #74709
    Purpose
    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of TFRC mRNA
    Depositor
    Eugene Yeo
    Article
    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30
    Insert
    U6 promoter driving sgRNA targeting the 3'UTR of TFRC mRNA
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    April 27, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 6922 results