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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,031 results for: nanoluc

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Showing: 701 - 720 of 1031 results

  1. G15-CASE

    Plasmid
    #168126
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein G15. Composed of the subunits G alpha 15 (GNA15) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Publication
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNA15, GNB3, GNG9 (GNA15, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at E244/N245 within GNA15
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168126-plasmid-map-sequence-id-332871

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  3. SMN2E7-Fluc-Nluc-reporter

    Plasmid
    #221005
    Purpose
    Transiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.
    Depositor
    Benjamin Blencowe, Mikko Taipale
    Publication
    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Fluc-SMN2E7-STOP-Nluc
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    221005-plasmid-map-sequence-id-437012

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  4. pcDNA3.1.Gα13-LgB106

    Plasmid
    #134364
    Purpose
    Nanoluc complementation assay. Expression of Gα13 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 106 and 107 of Gα13. Addition of the HA epitope at N term of Gα13.
    Depositor
    Julien Hanson
    Publication
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gα13-LgB106 (GNA13 Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134364-plasmid-map-sequence-id-306388

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  5. pcDNA3.1.Gα11-LgB97

    Plasmid
    #134361
    Purpose
    Nanoluc complementation assay. Expression of Gα11 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gα11. Addition of the HA epitope at N terminus of Gα11.
    Depositor
    Julien Hanson
    Publication
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gα11-LgB97 (GNA11 Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134361-plasmid-map-sequence-id-306399

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  6. nLuc-PTHR1

    Plasmid
    #162577
    Purpose
    Expresses N-terminally NanoLuc-Tagged PTHR1 in mammalian cells
    Depositor
    Sam Gellman
    Publication
    Yu et al ACS Chem Biol. 2022 Nov 18;17(11):3148-3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Parathyroid Hormone Receptor 1 (PTH1R Human)
    Tags
    Nanoluc
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    162577-plasmid-map-sequence-id-324482

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  9. pCDNA3.1-S-Luc_cyto

    Plasmid
    #113926
    Purpose
    Plasmid encoding the cytosolic localization of the chimeric SNAP-NanoLuc fusion S-Luc in mammalian cells for BRET
    Depositor
    Kai Johnsson
    Publication
    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S-Luc
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    113926-plasmid-map-sequence-id-222528

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  10. pCDNA3.1-S-Luc_mito

    Plasmid
    #113927
    Purpose
    Plasmid encoding the mitochondrial localization of the chimeric SNAP-NanoLuc fusion S-Luc in mammalian cells for BRET
    Depositor
    Kai Johnsson
    Publication
    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S-Luc_mito
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    113927-plasmid-map-sequence-id-222130

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  11. MLRV3:HRE-P53-MAPK/JNK-FOXO-TGFβ

    Plasmid
    #178320
    Purpose
    Multiplex luciferase reporter vector, with luciferase reporters for the pathways HRE, P53, AP-1, FOXO and SMAD.
    Depositor
    Koen Venken
    Publication
    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    HRE RedFirefly reporter
    P53 FLuc reporter
    AP-1 Renilla reporter
    FOXO NLuc Reporter
    SMAD GrRenilla reporter
    Use
    Luciferase and Synthetic Biology
    Expression
    Mammalian
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    178320-plasmid-map-sequence-id-363290

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  14. pNLF1W

    Plasmid
    #136404
    Purpose
    Expression of N-terminal NanoLuc luciferase fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.
    Depositor
    Sebastian Maurer
    Publication
    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    NanoLuc luciferase
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    136404-plasmid-map-sequence-id-271260

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  17. pAAV-anti-PDL1(KN035) PAGER(TF)

    Plasmid
    #230003
    Purpose
    Expresses anti-PDL1 PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)
    Depositor
    Alice Ting
    Publication
    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-
    Insert
    IL2SP-Aro6-KN035-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    Use
    AAV
    Expression
    Mammalian
    Mutation
    V360A/R361A on KORD
    Promoter
    CMV
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230003-plasmid-map-sequence-id-457206

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  18. pAAV-anti-GFP(LaG17) PAGER(TF)

    Plasmid
    #229997
    Purpose
    Expresses anti-GFP PAGER(TF) (high reversibility) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)
    Depositor
    Alice Ting
    Publication
    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-
    Insert
    IL2SP-Aro6-LaG17-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    Use
    AAV
    Expression
    Mammalian
    Mutation
    V360A/R361A on KORD
    Promoter
    CMV
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    229997-plasmid-map-sequence-id-457203

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Showing: 701 - 720 of 1031 results