We narrowed to 10,650 results for: t7

1. pT7-AsCas12a_crRNA-site3 (RTW549)

   Plasmid

   #160138

   Purpose

   T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #3

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Insert

   AsCas12a crRNA with spacer #3 (spacer=CTGATGGTCCATGTCTGTTACTC)
   

   Use

   In vitro transcription of sgrna from t7 promoter

   Promoter

   T7

   Available Since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Pr2-T7RNAP

   Plasmid

   #67739

   Purpose

   OR2-OR1-P2 promoter expressing T7 RNAP

   Depositor

   Richard Murray

   Article

   Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

   Insert

   UTR1-T7RNAP-T500
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   OR2-OR1-Pr2

   Available Since

   Aug. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZB573_pB1H1_T7-RNAP_LARP-I5CHO_1

   Plasmid

   #228508

   Purpose

   Bacterial Hybrid Driver Plasmid

   Depositor

   Timothy Whitehead

   Article

   Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

   Insert

   T7 RNAP, LARP-I5CHO (#1)
   

   Use

   Synthetic Biology

   Tags

   Umu Degron, GTG-start codon

   Mutation

   R378K; S430P, N433T, S633P, F849I, F880Y; W727G, …

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZB578_pB1H1_T7-RNAP_LARP-I

   Plasmid

   #228507

   Purpose

   Bacterial Hybrid Driver Plasmid

   Depositor

   Timothy Whitehead

   Article

   Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

   Insert

   T7 RNAP, LARP-I
   

   Use

   Synthetic Biology

   Tags

   Umu Degron, GTG-start codon

   Mutation

   R378K; S430P, N433T, S633P, F849I, F880Y; W727G, …

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28-5'TwStrep

   Plasmid

   #208620

   Purpose

   Empty vector for prokaryotic expression, insert a Twin Strep tag between NdeI and BamHI site of pET28a (+), both site retained, T7 tag replaced, BamHI site in-frame with Twin Strep tag.

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Tags

   Twin Strep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET E. coli expression vector with BioBrick polypromoter restriction sites (14-A)

   Plasmid

   #48307

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Nov. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET LIC cloning vector with BioBrick polycistronic restriction sites (9A)

   Plasmid

   #48283

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Nov. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGBR22-pGEM-T

   Plasmid

   #231055

   Purpose

   Expresses Montipora efflorescens GFP-like chromoprotein under T7 with a 6xHIS tag

   Depositor

   Josh Beckham

   Insert

   GFP-like chromoprotein
   

   Use

   Cloning

   Tags

   6x HIS

   Promoter

   T7

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETM6-G6-vioABE-4A6-vioC-3A2-vioD

   Plasmid

   #73440

   Purpose

   Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   PG6-vioABE-P4A6-vioC-P3A2-vioD
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   PG6, P4A6, and P3A2 (orthogonal T7-lac variants)

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSP2283

    Plasmid

    #70702

    Purpose

    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSP2266

    Plasmid

    #70705

    Purpose

    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBS-Cx43

    Plasmid

    #65439

    Purpose

    Cloning, T7 RNA expression

    Depositor

    Trond Aasen

    Article

    Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

    Insert

    Cx43 (GJA1 Human)
    

    Use

    T7 in vitro rna production

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET His6 TEV expression vector with BioBrick polypromoter restriction sites (14-B)

    Plasmid

    #48308

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET His6 Sumo TEV expression vector with BioBrick polypromoter restriction sites (14-S)

    Plasmid

    #48313

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6 and Sumo

    Expression

    Bacterial

    Available Since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET His6 msfGFP TEV cloning vector with BioBrick polycistronic restriction sites (9GFP)

    Plasmid

    #48287

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6 and msfGFP

    Expression

    Bacterial

    Available Since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET His6 TEV cloning vector with BioBrick polycistronic restriction sites (9B)

    Plasmid

    #48284

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET MBP TEV expression vector with BrioBrick polypromoter restriction sites (14-Q)

    Plasmid

    #48311

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    MBP

    Expression

    Bacterial

    Available Since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET His6 Sumo TEV cloning vector with BioBrick polycistronic restriction sites (9S)

    Plasmid

    #48291

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6 and Sumo

    Expression

    Bacterial

    Available Since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET His6 Thioredoxin TEV cloning vector with BioBrick polycistronic restriction sites (9T)

    Plasmid

    #48292

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6 and Thioredoxin

    Expression

    Bacterial

    Available Since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET His6 MBP TEV expression vector with BioBrick polypromoer restriction sites (14-C)

    Plasmid

    #48309

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6 and MBP

    Expression

    Bacterial

    Available Since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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