We narrowed to 15,989 results for: LEA

1. pRSF-G1TMSNKRS

   Plasmid

   #198323

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((trimethylsilyl)methyl)carbamoyl)-L-lysine (TMSNK), into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Ekanayake et al J Med Chem. 2023 Mar 15. doi: 10.1021/ac

   Insert

   tRNA synthetase
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBactin-Nedd1-mOrange2

   Plasmid

   #196860

   Purpose

   Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Nedd1-mOrange2 (Nedd1 Rat)
   

   Expression

   Mammalian

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-rox-inv[Talpha1-iCre-pA]-rox-LynGFP

   Plasmid

   #196874

   Purpose

   Neuron-specific expression of LynGFP reporter. Used in combination with Talpha1-Dre-pA plasmids

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   LynGFP
   

   Use

   Cre/Lox; Dre/rox

   Expression

   Mammalian

   Promoter

   Quimeric CAG

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-lox-inv[Talpha1-Dre-pA]-lox-Lyn-EGFP

   Plasmid

   #196876

   Purpose

   Neuron-specific expression of LynGFP reporter. Used in combination with Talpha1-iCre-pA plasmids

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   LynEGFP
   

   Use

   Cre/Lox; Dre/rox

   Expression

   Mammalian

   Promoter

   Quimeric CAG

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-DIO-inv[roxSTOProx-ZsGreen-bGH]

   Plasmid

   #196883

   Purpose

   Dual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotransfection reporter

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   roxSTOProx-ZsGreen-bGH
   

   Use

   Cre/Lox; Dre/rox

   Expression

   Mammalian

   Promoter

   Quimeric CAG

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-DIO-inv[roxSTOProx-Lyn-GFP-bGH]

   Plasmid

   #196884

   Purpose

   Dual Cre/Dre-dependent expression of LynGFP. Used as Cre-Dre cotransfection reporter

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   roxSTOProx-Lyn-GFP-bGH
   

   Use

   Cre/Lox; Dre/rox

   Expression

   Mammalian

   Promoter

   Quimeric CAG

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBactin-DIO-inv[roxSTOProx-ZsGreen-bGH]

   Plasmid

   #196886

   Purpose

   Dual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotransfection reporter

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   roxSTOProx-ZsGreen-bGH
   

   Use

   Cre/Lox; Dre/rox

   Expression

   Mammalian

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGBT9-FTS

   Plasmid

   #198526

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-FTS fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

   Insert

   FTS (AKTIP Human)
   

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGBT9-FHIP-L

   Plasmid

   #198528

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-FHIP-L fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

   Insert

   FHIP-L (FHIP1A Human)
   

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBridge-tyrosinase.σ3A

    Plasmid

    #197513

    Purpose

    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Inserts

    tyrosinase cytosolic tail
    AP-3 σ3A
    

    Tags

    GAL4-DNA binding domain fragment, HA tag, and nuc…

    Expression

    Yeast

    Mutation

    silent substitution in codon 2 of tyrosinase tail…

    Promoter

    ADH1 and MET25

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAc5.1B-lambdaN-HA-DmPAN2-del1047-1063-GSG-dsRNAres_Y

    Plasmid

    #148070

    Purpose

    Insect Expression of DmPAN2-del1047-1063-GSG-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

    Insert

    DmPAN2-del1047-1063-GSG-dsRNAres (PAN2 Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation compared the the sequence gei…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT7-EGFP-C1-HsNot1iso1-4x mut_R

    Plasmid

    #147371

    Purpose

    Mammalian Expression of HsNot1iso1-4xmut

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    HsNot1iso1-4xmut (CNOT1 Human)
    

    Expression

    Mammalian

    Mutation

    A deletion of 5 AA 822-827 SKMKPS-> T and one …

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7-EGFP-C1-HsNot1iso1-K1208AH1212AK1218A_R

    Plasmid

    #147372

    Purpose

    Mammalian Expression of HsNot1iso1-K1208AH1212AK1218A

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    HsNot1iso1-K1208AH1212AK1218A (CNOT1 Human)
    

    Expression

    Mammalian

    Mutation

    A deletion of 5 AA 822-827 SKMKPS-> T and one …

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT7-EGFP-C1-HsNot1iso1-K1208EH1212TK1218E_R

    Plasmid

    #147373

    Purpose

    Mammalian Expression of HsNot1iso1-K1208EH1212TK1218E

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    HsNot1iso1-K1208EH1212TK1218E (CNOT1 Human)
    

    Expression

    Mammalian

    Mutation

    A deletion of 5 AA 822-827 SKMKPS-> T and one …

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pT7-EGFP-C1-HsNot1iso1-P1209YP1257Y_R

    Plasmid

    #147374

    Purpose

    Mammalian Expression of HsNot1iso1-P1209YP1257Y

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    HsNot1iso1-P1209YP1257Y (CNOT1 Human)
    

    Expression

    Mammalian

    Mutation

    A deletion of 5 AA 822-827 SKMKPS-> T and one …

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT7-EGFP-C1-HsNot1iso1-P1257Y_R

    Plasmid

    #147375

    Purpose

    Mammalian Expression of HsNot1iso1-P1257Y

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    HsNot1iso1-P1257Y (CNOT1 Human)
    

    Expression

    Mammalian

    Mutation

    A deletion of 5 AA 822-827 SKMKPS-> T and one …

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAc5.1B-lambdaN-HA-DmNot1-K1277A-dsRNAres_R

    Plasmid

    #147359

    Purpose

    Insect Expression of DmNot1-K1277A-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    DmNot1-K1277A-dsRNAres (Not1 Fly)
    

    Expression

    Insect

    Mutation

    10 silent mutations and two non silent N605S, K75…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAc5.1B-lambdaN-HA-DmNot1-Q1271A-dsRNAres_R

    Plasmid

    #147360

    Purpose

    Insect Expression of DmNot1-Q1271A-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    DmNot1-Q1271A-dsRNAres (Not1 Fly)
    

    Expression

    Insect

    Mutation

    10 silent mutations and two non silent N605S, K75…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAc5.1B-lambdaN-HA-DmNot1-R1267A-dsRNAres_R

    Plasmid

    #147361

    Purpose

    Insect Expression of DmNot1-R1267A-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    DmNot1-R1267A-dsRNAres (Not1 Fly)
    

    Expression

    Insect

    Mutation

    10 silent mutations and two non silent N605S, K75…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLNHA-C1-HsNot1iso1_1-1089_K

    Plasmid

    #146804

    Purpose

    Mammalian Expression of HsNot1iso1_1-1089

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    HsNot1iso1_1-1089 (CNOT1 Human)
    

    Expression

    Mammalian

    Mutation

    Deletion of 5 AA compared to the sequence given b…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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