We narrowed to 19,373 results for: LEA

1. pYPQ230-E795L

   Plasmid

   #176890

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing LbCas12a with E795L mutation, without promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d

   Insert

   LbCas12a-E795L
   

   Use

   CRISPR; Gateway compatible lbcpf1 entry clone

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 3521 pET15b Bid p15

   Plasmid

   #8782

   Depositor

   Stanley Korsmeyer

   Article

   Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.

   Insert

   Bid p15 (Bid Mouse)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 6, 2005

   Availability

   Academic Institutions and Nonprofits only

3. pCI syn iGluSnFR

   Plasmid

   #106123

   Purpose

   Indicator for glutamate imaging (synapsin promoter)

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGluSnFR
   

   Use

   Tags

   Myc and PDGFR

   Expression

   Mammalian

   Mutation

   Promoter

   synapsin

   Available since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. WT (REP10)

   Plasmid

   #21368

   Depositor

   Gregory Germino

   Article

   Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

   Insert

   autosomal dominant polycystic kidney disease type I (PKD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 3xflag-APEX2-NES

   Plasmid

   #164623

   Purpose

   Expresses a nuclear exporting signal (NES) tagged with APEX2 and 3xflag

   Depositor

   Mark Cookson

   Article

   Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

   Insert

   NES
   

   Use

   Tags

   3xFLAG and APEX2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-ABKAR

   Plasmid

   #140051

   Purpose

   Genetically encoded FRET-based biosensor for monitoring AMPK activity. Also sensitive to phosphorylation by BRSK1/2 when expressed in neurons.

   Depositor

   Jin Zhang

   Article

   Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

   Insert

   ABKAR
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mRFP-LAP2β

   Plasmid

   #228029

   Purpose

   lentiviral construct for mRFP-LAP2β expression

   Depositor

   David Pellman

   Article

   Zhao et al Dev Cell. 2023 May 22;58(10):847-865.e10

   Insert

   LAP2b (Tmpo Rat)
   

   Use

   Lentiviral

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTMEM-PhoCl-Insert

   Plasmid

   #219674

   Purpose

   Caging at cytosolic N-ter of POI: TMEM-PhoCl-rtta3mScarlet

   Depositor

   Helge Ewers

   Article

   Kashyap et al Nat Methods. 2024 Mar 8. doi: 10.1038/s4

   Insert

   TMEM-PhoCl-rtTA3
   

   Use

   Tags

   PhoCl

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pET28_human-pro-IL-18_DeltaN24

   Plasmid

   #214327

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL18 (IL18 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Deletion of first 24 amino acids

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFastBac_human-pro-IL-18

    Plasmid

    #214337

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. BPK2301

    Plasmid

    #65778

    Purpose

    Human expression plasmid for S. thermophilus 1 Cas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-St1-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    St1Cas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSin-GFP-Fg

    Plasmid

    #174307

    Purpose

    Lentiviral expression of GFP

    Depositor

    Bruce Beutler

    Article

    Yue et al Science. 2021 May 14;372(6543). pii: 372

    Insert

    GFP (eGFP Aequorea victoria)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV E2F1 E132

    Plasmid

    #24224

    Purpose

    Mammalian expression of human E2F1 (DNA binding mutant) with HA tag

    Depositor

    Kristian Helin

    Article

    Helin et al Genes Dev. 1993 Oct . 7(10):1850-61.

    Insert

    E2F1 E132 (E2F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pDuRCC-N

    Plasmid

    #81194

    Purpose

    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please view depositor comments below and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pWZL N-Myc-hPOT1_deltaOB

    Plasmid

    #166408

    Purpose

    express hPOT1 ΔOB in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

    Insert

    POT1 ΔOB (POT1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    ΔOB

    Promoter

    Available since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21-pelB-LaG-18

    Plasmid

    #172763

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-18; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-18
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21-pelB-LaG-10

    Plasmid

    #172743

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-10; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-10
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTU398

    Plasmid

    #178980

    Purpose

    13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization)

    Depositor

    Stan Brouns

    Article

    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

    Insert

    gRAMP
    

    Use

    Tags

    Strep-Tag II SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV-syn-ChR2-Kv-P2A-H2b-mRuby2

    Plasmid

    #89570

    Purpose

    Channelrhodopsin localized to neuronal soma with simultaneous nuclear labeling

    Depositor

    McLean Bolton

    Article

    Baker et al Elife. 2016 Aug 15;5. pii: e14193. doi:

    Inserts

    Channelrhodopsin
    Histone 2B
    

    Use

    AAV

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    synapsin

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21-pelB-LaG-42

    Plasmid

    #172758

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-42; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-42
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge