We narrowed to 12,130 results for: LEA

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p667-UBC-DYRK3-V5-miniTurbo_IDG-K

Plasmid

#158190

Purpose

Gateway destination clone of DYRK3 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

DYRK3-V5-miniTurbo (DYRK3 Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

R293H- please see depositor comments

Promoter

Ubiquitin

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-Nr4a1sgRNA1

Plasmid

#160945

Purpose

Guide RNA 1 to generate Nr4a1 knockout by CRISPR

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nr4a1 gRNA 1 (Nr4a1 Mouse)

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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KRAS-V14I

Plasmid

#154427

Purpose

V14I Kras with Flag tag inserted into N-terminal domain between 6Xhis tag and TEV cleavage site.

Depositor

Kenneth Westover

Article

Bera et al J Biol Chem. 2019 Sep 20;294(38):13964-1

Insert

KRAS (KRAS Human)

Tags

6xHis-tag and TEV protease cleavage sequence

Expression

Bacterial

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-mCherry-FLARE-EKAR-EV

Plasmid

#123341

Purpose

Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

mCherry-mCherry-FLARE-EKAR-EV

Tags

6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-Venus-FLARE-AKAR

Plasmid

#123331

Purpose

Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Venus-Venus-FLARE-AKAR

Tags

6xHIS, T7 tag (gene 10 leader), Venus, and Xpress…

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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pGBW-m4252811

Plasmid

#153563

Purpose

Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;6xHis-003

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046548

Plasmid

#149130

Purpose

Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Tags

Cleavable thrombin;StrepII

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046386

Plasmid

#149089

Purpose

Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Tags

Cleavable thrombin;3xFLAG

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046380

Plasmid

#148977

Purpose

Yeast Expression plasmid for SARS-CoV-2 E (envelope)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Tags

Cleavable thrombin;MBP

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046194

Plasmid

#148979

Purpose

Yeast Expression plasmid for SARS-CoV-2 ORF7a protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF7a protein (ORF7a Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046181

Plasmid

#149090

Purpose

Yeast Expression plasmid for SARS-CoV-2 M (membrane)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Tags

Cleavable thrombin;StrepII

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available Since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046506

Plasmid

#145560

Purpose

Yeast Expression plasmid for SARS-CoV-2 ORF8 protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF8 protein (ORF8 Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available Since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046422

Plasmid

#145515

Purpose

Yeast Expression plasmid for SARS-CoV-2 ORF6 protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF6 protein (ORF6 Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Tags

Cleavable thrombin;3xFLAG

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available Since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046486

Plasmid

#145506

Purpose

Yeast Expression plasmid for SARS-CoV-2 ORF10 protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF10 protein (ORF10 Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available Since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046455

Plasmid

#145487

Purpose

Yeast Expression plasmid for SARS-CoV-2 E (envelope)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Tags

Cleavable thrombin;3xFLAG

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available Since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046351

Plasmid

#145449

Purpose

Yeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available Since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046589

Plasmid

#145450

Purpose

Yeast Expression plasmid for SARS-CoV-2 ORF6 protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF6 protein (ORF6 Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available Since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBB0323-TEV-His12

Plasmid

#137039

Purpose

expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0323 with C-terminal TEV-protease-cleavable His12

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Insert

AAC66700.1

Tags

His12 (TEV protease cleavable)

Expression

Bacterial

Mutation

wild type, full-length protein, contains signal p…

Promoter

T7

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pPRIME-CMV-GFP-shCrh1

Plasmid

#132709

Purpose

Encodes short hairpin RNA (shRNA) #1 that targets the 3’-untranslated region of the rat Crh gene

Depositor

Robert Messing

Article

Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

Insert

shCrh(1) (Crh Rat)

Use

Lentiviral

Promoter

CMV

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP2NhNABP1C

Plasmid

#128313

Purpose

Express chimeric protein hNABP2 N-terminus and hNABP1 C-terminus in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

chimeric protein hNABP2N+hNABP1C (NABP2, NABP1 Human)

Tags

His

Expression

Bacterial

Mutation

chimeric protein hNABP2N+hNABP1C, Y85C (please se…

Promoter

T7

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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