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We narrowed to 14,905 results for: sta

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Showing: 7181 - 7200 of 14905 results

  2. MADR pDonor smFP-HA (dark) WPRE

    Plasmid
    #131007
    Purpose
    MADR donor plasmid for FlpO+Cre- mediated insertion of SM_FP-HA, a cytoplasmic fluorescent protein which includes multiple HA epitope tags
    Depositor
    Joshua Breunig
    Publication
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    smFP-HA WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    HA - 10 total HA epitope tages
    Expression
    Mammalian
    Mutation
    "dark" variant with GGG fluorphore comp…
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    131007-plasmid-map-sequence-id-305185

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  3. pcDNA4/TO/Strep-HA-ZAKalpha_deltaS

    Plasmid
    #141197
    Purpose
    Expresses Strep-HA-ZAKalpha_deltaS in mammalian cells, can be used to make inducible cell lines
    Depositor
    Simon Bekker-Jensen
    Publication
    Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MAP3K20 (MAP3K20 Human)
    Tags
    Strep, HA
    Expression
    Mammalian
    Mutation
    deletion of amino acids 670-712
    Promoter
    CMV
    Available since
    June 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141197-plasmid-map-sequence-id-299550

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  4. pGBW-m4136460

    Plasmid
    #149532
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 E (envelope)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    6xHIS;Linker_GGSG;MBP;Linker_GGSG;Cleavable TEV
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149532-plasmid-map-sequence-id-313668

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  5. pGBW-m4095420

    Plasmid
    #149523
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 nsp3
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    d1-745 d1063-1945
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149523-plasmid-map-sequence-id-297203

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  6. pGBW-m4136454

    Plasmid
    #149529
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 13, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149529-plasmid-map-sequence-id-297077

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  7. pGBW-m4046953

    Plasmid
    #145764
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 ORF7b
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 ORF7b (ORF7b Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145764-plasmid-map-sequence-id-297851

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  8. pGBW-m4046801

    Plasmid
    #145741
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;Myc
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145741-plasmid-map-sequence-id-298377

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  9. pGBW-m4046890

    Plasmid
    #145740
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 ORF6 protein
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 ORF6 protein (ORF6 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145740-plasmid-map-sequence-id-297848

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  10. pcDNA3-Q842gp120-8his

    Plasmid
    #100932
    Purpose
    Mammalian expression plasmid for gp120 subunit from the Q842.d12 HIV-1 strain
    Depositor
    Erik Procko
    Publication
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HIV-1 (Q842.d12) Env
    Tags
    CD5 leader sequence and Purification tag after K5…
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene
    Promoter
    CMV
    Available since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    100932-plasmid-map-sequence-id-197608

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  13. pAAV-CamKIIa(0.4)-stChrimsonR-EGFP-P2A-PdCO-WPRE

    Plasmid
    #202198
    Purpose
    Expresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of minimal CamKIIa promotor
    Depositor
    Ofer Yizhar
    Publication
    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s
    Insert
    stChrimsonR, PdCO
    Use
    AAV
    Tags
    EGFP and Rho1D4
    Expression
    Mammalian
    Promoter
    CaMKIIα minimal promotor (0.4kb)
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202198-plasmid-map-sequence-id-397077

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  14. pGBW-m4046925

    Plasmid
    #145694
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145694-plasmid-map-sequence-id-313545

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  15. pCRI003-pGEM-LS-Bar-PgpdA-dSpCas9-VPR-TtrpC-LS

    Plasmid
    #140196
    Purpose
    Chromosomal integration of PgpdA-dSpCas9-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi vector.
    Depositor
    Yit Heng Chooi
    Publication
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dSpCas9-VPR
    Bar
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
    Use
    CRISPR and Synthetic Biology; Fungal genome integ…
    Tags
    VPR (VP64-p65-Rta) , NLS
    Promoter
    PgpdA and PtrpC
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    140196-plasmid-map-sequence-id-309706

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  16. pNW538_pAAVS1-pAP1(2)-FlpO-ABI-P2A-PYL-FlpO-CAG-U1-frt-STOP-frt-U2-GFP-P2A-luc2-ZeoR

    Plasmid
    #192939
    Purpose
    FlpO-based digitizer circuit driven by AP1 promoter
    Depositor
    Wilson Wong
    Publication
    Wong et al Nat Commun. 2021 Oct 22;12(1):6150. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    AP1(2)/minTK promoter
    FlpO-ABI
    PYL-FlpO
    CAG promoter
    frt-STOP-frt
    GFP
    luciferase
    pPGK
    Zeocin resistance
    BFP
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available since
    Dec. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    192939-plasmid-map-sequence-id-380399

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  17. MADR pDonor TagBFP2-V5-HRAS G12VWPRE

    Plasmid
    #129420
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion
    Depositor
    Joshua Breunig
    Publication
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    TagBFP2-V5-HRAS G12V (HRAS Entacmaea quadricolor (TagBFP), Human)
    Use
    Mosaic analysis for dual recombinase-mediated cas…
    Tags
    TagBFP2-V5 tag
    Mutation
    G12V
    Promoter
    none (3x polyA to mitigate episomal expression)
    Available since
    Sept. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    129420-plasmid-map-sequence-id-257068

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  18. K15-TK/pGL3

    Plasmid
    #44267
    Depositor
    George Cotsarelis
    Publication
    Ito et al Nat Med. 2005 Dec;11(12):1351-4. Epub 20
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    K15 promoter (-4.8) (Krt15 Mouse)
    Use
    Mouse Targeting; Thymidine kinase
    Tags
    HSV-1 TK
    Expression
    Mammalian
    Mutation
    Contains the murine K15 promoter (-4.8) fragment
    Promoter
    murine K15 promoter (-4.8) fragment
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 7181 - 7200 of 14905 results