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pWay21-MKP-1DCH2AB

Plasmid

#13472

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1DCH2AB (Dusp1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of MKP-1 CH2AB; contains aa 137-367

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pDONR221-rap1-K462-463-651R

Plasmid

#84564

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

K462-463-651R

Promoter

none

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221-rap1-K43-117-118R

Plasmid

#84565

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

K43-117-118R

Promoter

none

Available Since

Oct. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pMT3-RhoAC

Plasmid

#100032

Purpose

This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with adenylyl cyclase activity.

Depositor

Daniel Oprian

Article

Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

Insert

RhoAC

Tags

1D4 (TETSQVAPA)

Expression

Mammalian

Mutation

E497K/C566D

Promoter

Adenovirus major late promoter

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pGS1212

Plasmid

#63785

Purpose

Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Strep-tag II followed by URA3 selection marker

Depositor

Monika Golas, Bjoern Sander

Article

Rai et al Mol Biotechnol. 2014 Jun 27.

Insert

eGFP-Strep-tag II followed by URA3 selection marker

Use

E. coli cloning vector

Tags

eGFP-Strep-tag II

Promoter

none

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pUAST YFP Rab6 DN

Plasmid

#58448

Purpose

expression of drosophila YFP-Rab6 dominant negative mutant

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab6 (Rab6 Fly)

Tags

YFP

Expression

Insect

Mutation

T26N (dominant negative)

Promoter

GAL4

Available Since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
pUAST YFP Rab6 WT

Plasmid

#58447

Purpose

expression of drosophila YFP-Rab6

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab6 (Rab6 Fly)

Tags

YFP

Expression

Insect

Promoter

GAL4

Available Since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1DCH2A

Plasmid

#13470

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1DCH2A (Dusp1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of MKP-1 CH2A; contains aa 47-367

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pLEX_970_puro_DEST_V5_LUC

Plasmid

#45290

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

Luciferase

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

June 27, 2013

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab1 DN

Plasmid

#37682

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab1 (Rab1 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

S25N (dominant negative)

Promoter

GAL4

Available Since

Nov. 16, 2012

Availability

Academic Institutions and Nonprofits only
pRK5-HA-SH3BP4 W92A

Plasmid

#46893

Purpose

expresses HA tagged SH3BP4 W92A mutant in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2012 Jun 29;46(6):833-46. doi:

Insert

SH3BP4 W92A (SH3BP4 Human)

Tags

HA

Expression

Mammalian

Mutation

W92A (mutated SH3 domain)

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pUASP YFP Rab7 DN

Plasmid

#37703

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab7 (Rab7 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

T22N (dominant negative)

Promoter

GAL4

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
pTJK6 (miR-513a WT OE in pWCC52)

Plasmid

#254385

Purpose

Express human miR-513a

Depositor

Curt Civin

Article

Kim et al FEBS Open Bio. 2026 Mar 8. doi: 10.1002/

Insert

miR-513a-1 (MIR513A1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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Modified pYTK048

Plasmid

#254021

Purpose

It was constructed by reversing the orientation of the BsaI sites in the original pYTK048 and is used for the construction of connectors.

Depositor

Dae-Hee Lee

Article

Seong et al Trends Biotechnol. 2026 Jan 14:S0167-779

Insert

Spacer

Use

Synthetic Biology

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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[#GS31-LV] CMV-MitoTRACER-Donor

Plasmid

#253759

Purpose

Lentiviral MitoTRACER donor construct under CMV Promoter

Depositor

Simon Grelet

Article

Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

Insert

MitoTRACER Donor

Tags

GFP11 and HA Tag

Promoter

CMV

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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[#GS32-LV] EF1a-MitoTRACER-Donor

Plasmid

#253760

Purpose

Lentiviral MitoTRACER donor construct under EF1a Promoter

Depositor

Simon Grelet

Article

Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

Insert

MitoTRACER-Donor

Tags

GFP11 and HA Tag

Promoter

EF1a

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV_CAG(del)-FLEx(loxP)-ARch3.0:EYFP

Plasmid

#248667

Purpose

Expresses Archaerhodopsin3 tagged to eYFP in Cre-expressing cells under the CAG promoter for neuronal inhibition.

Depositor

Gordon Fishell

Article

Mazo et al Res Sq [Preprint]. 2025 Jun 12:rs.3.rs-6

Insert

Arch3.0-EYFP

Use

AAV

Tags

eYFP

Expression

Mammalian

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV_CAG(del)--FLEx(loxP)-splitTVA:HA

Plasmid

#248665

Purpose

Helper virus for monosynaptic tracing; Expresses TVA and an HA tag in Cre-expressing cells under the CAG promoter. To be co-injected with Rabies Glycoprotein (G) viruses.

Depositor

Gordon Fishell

Article

Mazo et al Res Sq [Preprint]. 2025 Jun 12:rs.3.rs-6

Insert

TVA

Use

AAV

Tags

HA

Expression

Mammalian

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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TEVESNp_pRK793

Plasmid

#247042

Purpose

pRK793 (from David Waugh, Addgene plasmid #8827) backbone with ENLYFES cut site and TEVESNp insert.

Depositor

Carl Denard

Article

Nelson et al ACS Synth Biol. 2025 Jun 20;14(6):2241-2

Insert

MBP-ENLYFES-7xHIS-TEVESNp

Tags

His and MBP

Expression

Bacterial

Mutation

S120C, D148R, Tl73A, N174R, N176 V, Nl77K, G213A,…

Available Since

Feb. 18, 2026

Availability

Academic Institutions and Nonprofits only

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P1K-3HA-Atg8-MET15

Plasmid

#207032

Purpose

Expression of 3HA-Atg8.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xHA

Expression

Yeast

Promoter

pATG8

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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