We narrowed to 150,751 results for: ins

1. R2pH-LAMP-FRB

   Plasmid

   #225138

   Purpose

   R2pH-LAMP1-labelled lysosomes with FRB

   Depositor

   Massimiliano Stagi

   Article

   Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

   Insert

   Lamp1 (Lamp1 Mouse)
   

   Expression

   Mammalian

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-NPR4 (SA binding domain)

   Plasmid

   #226885

   Purpose

   Expresses the Salicylic acid binding core (SBC) of NPR4 in E.coli, in an insoluble form

   Depositor

   Ning Zheng

   Article

   Wang et al Nature. 2020 Oct;586(7828):311-316. doi:

   Insert

   The Salicylic acid (SA) binding domain of NPR4 (NPR4 Mustard Weed)
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   NPR4 SA-binding core (residues 373-516), with a s…

   Promoter

   T7

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMAL-MBP-KRas-Q61L

   Plasmid

   #214300

   Purpose

   Express constitutively active KRas fused with MBP

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Insert

   Constitutively active KRas (KRAS Human)
   

   Tags

   Maltose-binding protein (MBP)

   Expression

   Bacterial

   Mutation

   KRas-Q61L

   Promoter

   Tac

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMAL-MBP-KRas-S17N

   Plasmid

   #214301

   Purpose

   Express dominant negative KRas fused with MBP

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Insert

   Dominant negative KRas (KRAS Human)
   

   Tags

   Maltose-binding protein (MBP)

   Expression

   Bacterial

   Mutation

   KRas-S17N

   Promoter

   Tac

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR-Sensor_P2A_SLC2A4

   Plasmid

   #221429

   Purpose

   Gateway entry clone encoding a polyprotein that is cleaved to FRET glucose sensor and SLC2A4 proteins

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   Sensor_P2A_SLC2A4 (SLC2A4 Synthetic)
   

   Expression

   Mammalian

   Available Since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. TH1203-pOCC177_pOEM1-N-HIS-MBP-PS-TAF15_opt-TEV-SNAP

   Plasmid

   #221891

   Purpose

   Shuttle vector for baculovirus production, using FlashBac bacmid

   Depositor

   Anthony Hyman

   Article

   Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

   Insert

   TAF15 (TAF15 Human)
   

   Tags

   6xHis-MBP-PreScission and TEV-SNAP-tag

   Expression

   Insect

   Mutation

   wt

   Promoter

   PH promoter

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-XPACK-DNAJC5 (L115R)- HaloTag

   Plasmid

   #205712

   Purpose

   Mammalian expression of XPACK-DNAJC5 (L115R)-HaloTag

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   DNAJC5 (DNAJC5 Human)
   

   Tags

   HaloTag and XPACK (GCINSKRKD)

   Expression

   Mammalian

   Mutation

   L115R

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Recruited polymerase expression - pCMV-T7-B103-CC(N5) (LM2946)

   Plasmid

   #208974

   Purpose

   Recruited B103 DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   B103-BPNLS-CC(N5)
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Recruited polymerase expression - pCMV-T7-Phi29-CC(N5) (LM2726)

   Plasmid

   #208971

   Purpose

   Recruited Phi29 DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Phi29-BPNLS-CC(N5)
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Phi29(-exo;D169A)

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Recruited polymerase expression - pCMV-T7-Phi29(+exo)-CC(N5) (LM2761)

    Plasmid

    #208970

    Purpose

    Recruited Phi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Phi29(+exo)-BPNLS-CC(N5)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV_PGK_3xHA-LTB4R(T308A)

    Plasmid

    #208092

    Purpose

    Mammalian expression of the human LTB4 receptor LTB4R with the T308A mutation with an N-terminal 3x HA tag.

    Depositor

    Sean R Collins

    Article

    Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

    Insert

    3xHA-LTB4R(T308A) (LTB4R Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    T308A

    Promoter

    PGK

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV_PGK_3xHA-LTB4R(S310A)

    Plasmid

    #208093

    Purpose

    Mammalian expression of the human LTB4 receptor LTB4R with the S310A mutation with an N-terminal 3x HA tag.

    Depositor

    Sean R Collins

    Article

    Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

    Insert

    3xHA-LTB4R(S310A) (LTB4R Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    S310A

    Promoter

    PGK

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV_PGK_3xHA-LTB4R(T308A,S310A)

    Plasmid

    #208094

    Purpose

    Mammalian expression of the human LTB4 receptor LTB4R with the T308A and S310A mutations with an N-terminal 3x HA tag.

    Depositor

    Sean R Collins

    Article

    Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

    Insert

    3xHA-LTB4R(T308A,S310A) (LTB4R Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    T308A, S310A

    Promoter

    PGK

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. ACVR1-TASA-R206H

    Plasmid

    #206182

    Purpose

    in vitro transcription of RNA encoding human ACVR1 with mutations S190A, S194A, and R206H

    Depositor

    Mary Mullins

    Article

    Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

    Insert

    activin A receptor type 1 (ACVR1 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG tag

    Mutation

    S190A, S194A, R206H

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. ACVR1-TSAA-R206H

    Plasmid

    #206183

    Purpose

    in vitro transcription of RNA encoding human ACVR1 with mutations S192A, S194A, and R206H

    Depositor

    Mary Mullins

    Article

    Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

    Insert

    activin A receptor type 1 (ACVR1 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG tag

    Mutation

    S192A, S194A, R206H

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. ACVR1-VSAS-R206H

    Plasmid

    #206185

    Purpose

    in vitro transcription of RNA encoding human ACVR1 with mutations T189V, S192A, and R206H

    Depositor

    Mary Mullins

    Article

    Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

    Insert

    activin A receptor type 1 (ACVR1 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG tag

    Mutation

    T189V, S192A, R206H

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. ACVR1-VASS-R206H

    Plasmid

    #206186

    Purpose

    in vitro transcription of RNA encoding human ACVR1 with mutations T189V, S190A, and R206H

    Depositor

    Mary Mullins

    Article

    Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

    Insert

    activin A receptor type 1 (ACVR1 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG tag

    Mutation

    T189V, S190A, R206H

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET32a-hEPGN

    Plasmid

    #199232

    Purpose

    Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

    Depositor

    Nilson Zanchin

    Article

    Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

    Insert

    Epithelial mitogen isoform 6 (EPGN Human)
    

    Tags

    TrxA-6xHis-S-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSF-G1TMSNKRS

    Plasmid

    #198323

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((trimethylsilyl)methyl)carbamoyl)-L-lysine (TMSNK), into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Ekanayake et al J Med Chem. 2023 Mar 15. doi: 10.1021/ac

    Insert

    tRNA synthetase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. EGFP-MYO10-FERM-ITGBD

    Plasmid

    #194855

    Purpose

    Express EGFP-MYO10 with mutations (S2001_F2002insA/T2009D) that interfere with ITGB binding in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

    Insert

    MYO10 S2001_F2002insA/T2009D (MYO10 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    MYO10 has the following mutations S2001_F2002insA…

    Promoter

    CMV

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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