We narrowed to 11,390 results for: mpl

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pAG415Gal_Gcn4 SATtoG KtoRGFP

Plasmid

#233006

Purpose

Galactose iduced expression of Gcn4 SATtoG KtoRGFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 SATtoG KtoR

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 ILVtoED solvvol

Plasmid

#231858

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED solvvol

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED solvvol

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

Promoter

T7

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCA528 IST1 L328D,L355A (316-366)(C-Cys)

Plasmid

#193037

Purpose

Bacterial expression plasmid for IST1 (316-366) with C-terminal Cysteine for fluorescence polarization binding assays. Contains L328D and L355A mutations that diminishes binding to MIT domains.

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

IST1 (IST1 Human)

Tags

6xHis-Sumo

Expression

Bacterial

Mutation

L328D, L355A, Residues 316-366, Non-native C-term…

Promoter

T7

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pEF2-FL-hTyro_gR1

Plasmid

#225995

Purpose

Expresses a chimeric contruct having N-terminal human Tyro3 and C-terminal human Interferon Gamma Receptor

Depositor

Raymond Birge, Sergei Kotenko

Article

Tsou et al J Biol Chem. 2014 Sep 12;289(37):25750-6

Insert

human Tyro3 N-Terminal human Interferon Gamma R1 Receptor C-Terminal fusion (TYRO3, IFNGR1 Human)

Expression

Mammalian

Promoter

EF1-a

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pEF2-FL-hAxl_gR1

Plasmid

#225994

Purpose

Expresses a chimeric contruct having N-terminal human Axl and C-terminal human Interferon Gamma Receptor

Depositor

Raymond Birge, Sergei Kotenko

Article

Tsou et al J Biol Chem. 2014 Sep 12;289(37):25750-6

Insert

human Axl N-Terminal human Interferon Gamma R1 Receptor C-Terminal fusion (AXL, IFNGR1 Human)

Expression

Mammalian

Promoter

EF1-a

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-hWIPI3-3×FLAG

Plasmid

#215511

Purpose

Expresses FLAG tagged human WIPI3.

Depositor

Noboru Mizushima

Article

Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

Insert

WD-repeat protein interacting with phosphoinositides 3 (WDR45B Human)

Use

Retroviral

Tags

3×FLAG

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA.5.1-FHS-TNRC6A-SD-S1332D/S1346D/S1616D/S1691D

Plasmid

#215910

Purpose

Expression of TNRC6A-silencing domain (SD) with S1332D/S1346D/S1616D/S1691D mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

Flag-HA-SBP

Expression

Mammalian

Mutation

contain aa1224-1709, S1332D, S1346D, S1616D, S169…

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA.5.1-FHS-TNRC6A-SD-S1332A/S1346A/S1616A/S1691A

Plasmid

#215911

Purpose

Expression of TNRC6A-silencing domain (SD) with S1332A/S1346A/S1616A/S1691A mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

Flag-HA-SBP

Expression

Mammalian

Mutation

contain aa1224-1709, S1332A, S1346A, S1616A, S169…

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-S1332A/S1346A/S1616A/S1691A

Plasmid

#215905

Purpose

Expression of TNRC6A-silencing domain (SD) with S1332A/S1346A/S1616A/S1691A mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1224-1709, S1332A, S1346A, S1616A, S169…

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor-FMR1-CGG-BsmBI

Plasmid

#222966

Purpose

0CGG with BsmBI site for inserting CGG repeats

Depositor

Xinyu Zhao

Article

Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

Insert

FMR1 homologous template (no CGG repeats) with cloning site (FMR1 Human)

Use

CRISPR

Mutation

CGG repeats deleted from 5' UTR, BsmBI site …

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1297-AAV-EFSNC-dCjCas9-HP1b(79-173)

Plasmid

#223142

Purpose

Expression of truncated HP1b with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1b (CBX1 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 79-173

Promoter

EF1a

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1298-AAV-EFSNC-dCjCas9-HP1bNH(111-173)

Plasmid

#223143

Purpose

Expression of truncated HP1b with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1b (CBX1 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 111-173

Promoter

EF1a

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1308-AAV-EFSNC-dSaCas9-HP1bNH(111-173)

Plasmid

#223153

Purpose

Expression of truncated HP1b with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1b (CBX1 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 111-173

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1313-AAV-EFSNC-dSaCas9-MECP2(204-310)

Plasmid

#223158

Purpose

Expression of truncated MECP2 with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1307-AAV-EFSNC-dSaCas9-HP1b(79-173)

Plasmid

#223152

Purpose

Expression of truncated HP1b with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1b (CBX1 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 79-173

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-NOT1 isoform C-mCherry-PH (EB5)

Plasmid

#206474

Purpose

NOT1 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

NOT1 (Not1 Fly)

Tags

mcherry PH

Expression

Insect

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-Oskar 388-606 (OSK domain) (HK75)

Plasmid

#206463

Purpose

Oskar 388-606 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Oskar 388-606 (osk Fly)

Tags

PH mcherry

Expression

Insect

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-EGFP-Aubergine 4R-K (R11K/R13K/R15K/R17K) (HK121)

Plasmid

#206433

Purpose

Aubergine expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Aubergine (aub Fly)

Tags

EGFP

Expression

Insect

Mutation

R11K/R13K/R15K/R17K

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-mEGFP-NOT1 isoform C (HK53)

Plasmid

#206440

Purpose

NOT1 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

NOT1 (Not1 Fly)

Tags

mEGFP

Expression

Insect

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-mEGFP-NOT2 isoform A (HK54)

Plasmid

#206441

Purpose

NOT1 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

NOT1 (Not1 Fly)

Tags

mEGFP

Expression

Insect

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-mCherry-Bruno isoform A (JM78)

Plasmid

#206446

Purpose

Bruno expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Bruno (aret Fly)

Tags

mCherry

Expression

Insect

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mEGFP-Bruno isoform A (JM165)

Plasmid

#206452

Purpose

Bruno expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Bruno (aret Fly)

Tags

PH mEGFP

Expression

Insect

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-Oskar 139-606 isoform A (Short Oskar) (HK195)

Plasmid

#206460

Purpose

Oskar 139-606 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Oskar 139-606 (osk Fly)

Tags

PH mcherry

Expression

Insect

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Beta1 Q280* full-length

Plasmid

#221402

Purpose

Mammalian expression of human integrin beta1 Q280* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 Q280* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Beta1 K418* full-length

Plasmid

#221403

Purpose

Mammalian expression of human integrin beta1 K418* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 K418* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Beta1 N391* full-length

Plasmid

#221404

Purpose

Mammalian expression of human integrin beta1 N391* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 N391* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Beta1 Q280* K418* full-length

Plasmid

#221406

Purpose

Mammalian expression of human integrin beta1 Q280* K418* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 Q280* K418* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Beta1 N562* full-length

Plasmid

#221405

Purpose

Mammalian expression of human integrin beta1 N562* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 N562* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Beta1 N391* N562* full-length

Plasmid

#221407

Purpose

Mammalian expression of human integrin beta1 N391* N562* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 N391* N562* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB1.HMBP.PrS.EZH2-mt1

Plasmid

#220239

Purpose

Expression of an EZH2 mutant in insect cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 mt 1 (EZH2 Human)

Tags

6x His, MBP

Expression

Insect

Mutation

R27A, R31A, F165A

Promoter

Polyhedrin

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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