We narrowed to 14,321 results for: cas9

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pGEM-T Easy-AtpU6-26

Plasmid

#160218

Purpose

AtpU6-26 Golden Gate level 0 piece to express gRNAs

Depositor

Matias Kirst

Publication

Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

Insert

pAtU6-26 promoter

Use

Golden gate level 0 piece to express grnas

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMC0x_gRNA_Pos5_Ptet

Plasmid

#179336

Purpose

gRNA Position Vector 5, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

Depositor

Anke Becker

Publication

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptet gRNA with sfGFP dropout

Use

CRISPR

Available since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pMC0x_gRNA_Pos4_Ptet

Plasmid

#179335

Purpose

gRNA Position Vector 4, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

Depositor

Anke Becker

Publication

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptet gRNA with sfGFP dropout

Use

CRISPR

Available since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pMC0x_gRNA_Pos5b2_Ptet

Plasmid

#179342

Purpose

gRNA Position Vector 5b2, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

Depositor

Anke Becker

Publication

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptet gRNA with sfGFP dropout

Use

CRISPR

Available since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pRU206

Plasmid

#167693

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Publication

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUBQ4-2::asCpf1

Use

CRISPR

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRU322

Plasmid

#167683

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Publication

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::asCas9

Use

CRISPR

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-TetR-Rad18UBD

Plasmid

#119010

Purpose

Mammalian expression of a fusion protein of single chain Tet repressor with the human Rad18 Ubiquitin binding domain and BFP expression

Depositor

Ralf Kuehn

Publication

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

TetR

Expression

Mammalian

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19-EAMP

Plasmid

#138265

Purpose

mCherry acceptor plasmid with iCas9 target site upstream of mCherry to be used in plasmid to plasmid integration reporter assay.

Depositor

Xiao Wang

Publication

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

iCas9 target-mCherry

Use

CRISPR

Expression

Mammalian

Promoter

EF1a-HTLV

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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PABE-2

Plasmid

#115623

Purpose

Targeted A to G in wheat plants or monocotyledons protoplasts

Depositor

Caixia Gao

Publication

Li et al Genome Biol. 2018 May 29;19(1):59. doi:

Insert

wtTadA-TadA7.10-nCas9-NLS

Use

CRISPR

Expression

Plant

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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PABE-1

Plasmid

#115622

Purpose

Targeted A to G in wheat plants or monocotyledons protoplasts

Depositor

Caixia Gao

Publication

Li et al Genome Biol. 2018 May 29;19(1):59. doi:

Insert

NLS-wtTadA-TadA7.10-nCas9-NLS

Use

CRISPR

Expression

Plant

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-stop

Plasmid

#113745

Purpose

Contains three stop codons in every frame to ensure a premature stop codon when introduced into the genome via homology-directed repair. Gateway multisite entry clone.

Depositor

Magdalena Bezanilla

Publication

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

stop cassette

Use

CRISPR and Gateway Cloning

Mutation

Added 3 stop codons to the original sequence; see…

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pENTR-R4R3-3xmEGFP-C

Plasmid

#113751

Purpose

Gateway multi-site entry clone for second position 3x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Publication

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mEGFP-mEGFP-mEGFP

Use

CRISPR and Gateway Cloning

Tags

3xmEGFP

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pENTR-R4R3-3xmRuby-N

Plasmid

#113754

Purpose

Gateway multi-site entry clone for second position 3x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Publication

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mRuby2-mRuby2-mRuby2

Use

CRISPR and Gateway Cloning

Tags

3xmRuby2

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAP1716-1

Plasmid

#105245

Purpose

human eGFP with homology arms for Lamin A/C

Depositor

Geraldine Seydoux

Publication

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

eGFP with homology arms for Lamin A/C (LMNA Human)

Expression

Bacterial

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAP1892-1

Plasmid

#105247

Purpose

human GFP11 with tagRFP 33bp downstream in Lamin A/C

Depositor

Geraldine Seydoux

Publication

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

Insertion of GFP11 at cut tagRFP 33bp downstream in Lamin A/C (LMNA Human)

Expression

Bacterial

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCFD3-MS2tail-Scaffold

Plasmid

#78906

Purpose

Vector for expressing a single sgRNA with Scaffold MS2 tail, under U6:3 promoter

Depositor

Norbert Perrimon

Publication

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available since

Sept. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pAWp9-5

Plasmid

#73856

Purpose

pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg2-U6p-GFP-sg1

Depositor

Timothy Lu

Publication

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Insert

UBCp-RFP-CMVp-GFP-U6p-RFP-sg2-U6p-GFP-sg1

Use

Lentiviral

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
sgRNA2_NANOG

Plasmid

#64154

Purpose

Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA2_NANOG (NANOG Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA3_NANOG

Plasmid

#64155

Purpose

Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA3_NANOG (NANOG Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA4_NANOG

Plasmid

#64156

Purpose

Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA4_NANOG (NANOG Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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