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pDECKO-mCherry MALAT1_Enhancer.2

Plasmid

#78537

Purpose

Experimental plasmid

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 and U6 (for expressing sgRNA)

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pEMS2023

Plasmid

#49130

Purpose

AAV plasmid with hs1218 promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-hs1218-iCre

Use

AAV

Expression

Mammalian

Promoter

hs1218

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2024

Plasmid

#49131

Purpose

AAV plasmid with hs671 promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-hs671-iCre

Use

AAV

Expression

Mammalian

Promoter

hs671

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pSG5 N-HA SADBs shRNA resistant

Plasmid

#67157

Purpose

N-terminally HA tagged mouse SADBs containing substitutions providing resistance to an shRNA

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

Brsk1 (Brsk1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Nucleotide 294 C-T Nucleotide 297 C-G (shRNA resi…

Promoter

SV40 promoter

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
mCardinal-Cx26-7

Plasmid

#56156

Purpose

Localization: Gap Junctions, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Cx26

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mCardinal-CAF1-C-10

Plasmid

#56153

Purpose

Localization: Chromatin Assembly Factor 1, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

CAF1

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
tdminiSOG-Tubulin-6

Plasmid

#57808

Purpose

Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Tubulin (TUBA1B Human)

Tags

tdminiSOG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
PA-mCherry-miniSOG-Vinculin-23

Plasmid

#57801

Purpose

Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Vinculin (VCL Human)

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
miniSOG-Vinculin-23

Plasmid

#57780

Purpose

Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Vinculin (VCL Human)

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mPapaya-CENPB-N-22

Plasmid

#56344

Purpose

Localization: Nucleus/Centromeres, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

CENPB (CENPB Human)

Tags

mPapaya

Expression

Mammalian

Mutation

aa 1-169 of CENPB (DNA Binding Domain)

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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mPapaya-Vimentin-7

Plasmid

#56646

Purpose

Localization: Intermediate Filaments, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Vimentin (VIM Human)

Tags

mPapaya

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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mPapaya-Alpha-Actinin-19

Plasmid

#56640

Purpose

Localization: Alpha Actinin, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Alpha-Actinin (ACTN1 Human)

Tags

mPapaya

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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mPapaya-Zyxin-6

Plasmid

#56647

Purpose

Localization: Focal Adhesions, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Zyxin (ZYX Human)

Tags

mPapaya

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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mKO-Vinculin-23

Plasmid

#57852

Purpose

Localization: Focal Adhesions, Excitation: 548, Emission: 561

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Vinculin (VCL Human)

Tags

mKO

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only
BacPAK Myc-Adrm1

Plasmid

#19420

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

Insert

adhesion regulating molecule 1 (ADRM1 Human)

Tags

His and Myc

Expression

Insect

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pIS58 TIP120A 3'UTR

Plasmid

#14501

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

TIP120A 3'UTR (CAND1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
Toronto KnockOut version 3 library (TKOv3)

Pooled Library

#90294

Purpose

Optimized library offering improved accuracy, efficiency, and scalability for CRISPR screens compared to TKOv1

Depositor

Jason Moffat

Article

Hart et al G3 (Bethesda). 2017 Aug 7;7(8):2719-2727

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only
pEJS2602_AAV-Nme2-ABE8e-i1(V106W)-U6-2xSapI

Plasmid

#201684

Purpose

All-in-one AAV plasmid expressing Nme2-ABE8e-i1(V106W) and U6 sgRNA cassette. SapI enables cloning new spacers.

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-ABE8e-i1 (V106W)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U1a

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pEF1a-hMLH1NTD-NLS

Plasmid

#174826

Purpose

Mammalian expression of human MLH1NTD-NLS (codon optimized)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

human MLH1_NTD-NLS (codon optimized)

Tags

SV40 bpNLS

Expression

Mammalian

Mutation

truncation of residues 1-335

Promoter

EF1a

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-DRD2-cpHalo∆-mEGFP

Plasmid

#194679

Purpose

CMV driven expression of D2R GPCR recorder in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

DRD2_cpHalo∆

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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