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HMGN1_pLENTI-CAG-IRES-GFP

Plasmid

#176995

Purpose

Mammalian lentiviral expression vector encoding HMGN1

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

HMGN1 (HMGN1 Human)

Use

Lentiviral

Promoter

CAG

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pST1374-GCN4-D10A

Plasmid

#113022

Purpose

Expresses GCN4-D10A in mammalian cells

Depositor

Xingxu Huang

Article

Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

Insert

GCN4-D10A (GCN4 Budding Yeast, S. pyogenes)

Expression

Mammalian

Mutation

D10A

Promoter

CMV

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pScarlessHD-C-3xVHH05-DsRed

Plasmid

#171580

Purpose

For scarless insertion of 3xVHH05 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

3xVHH05 (UBE2J1 Human)

Expression

Insect

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-human LRRC33

Plasmid

#111602

Purpose

Express human LRRC33 in 293T cells

Depositor

Timothy Springer

Article

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

human LRRC33 (NRROS Human)

Tags

myc/his tag

Expression

Mammalian

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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sg_Brd9_i5-ipUSEPR-TR657

Plasmid

#228922

Purpose

Knockdown of Brd9 in mammalian cells

Depositor

Sika Zheng

Article

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

sg_Brd9_i5 (Brd9 Mouse)

Use

Lentiviral

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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sg_Gemin2_i1-ipUSEPR-TR657

Plasmid

#228919

Purpose

Knockdown of Gemin2 in mammalian cells

Depositor

Sika Zheng

Article

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

sg_Gemin2_i1 (Gemin2 Mouse)

Use

Lentiviral

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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sg_Gemin2_i5-ipUSEPR-TR657

Plasmid

#228920

Purpose

Knockdown of Gemin2 in mammalian cells

Depositor

Sika Zheng

Article

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

sg_Gemin2_i5 (Gemin2 Mouse)

Use

Lentiviral

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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sg_Brd9_i2-ipUSEPR-TR657

Plasmid

#228921

Purpose

Knockdown of Brd9 in mammalian cells

Depositor

Sika Zheng

Article

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

sg_Brd9_i2 (Brd9 Mouse)

Use

Lentiviral

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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sg_Snrpa_i2-ipUSEPR-TR657

Plasmid

#228923

Purpose

Knockdown of Snrpa in mammalian cells

Depositor

Sika Zheng

Article

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

sg_Snrpa_i2 (Snrpa Mouse)

Use

Lentiviral

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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sg_Snrpa_i4-ipUSEPR-TR657

Plasmid

#228924

Purpose

Knockdown of Snrpa in mammalian cells

Depositor

Sika Zheng

Article

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

sg_Snrpa_i4 (Snrpa Mouse)

Use

Lentiviral

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-nArgBP2ΔNSE

Plasmid

#74515

Purpose

Express GFP-nArgBP2ΔNSE fusion protein in mammalian cells. nArgBP2ΔNSE is the mutant without the neuronal specific exon of nArgBP2.

Depositor

Guoping Feng

Article

Zhang et al J Neurosci. 2016 Feb 17;36(7):2247-60. d

Insert

nArgBP2delNSE (Sorbs2 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-MUTED

Plasmid

#164629

Purpose

Expresses MUTED in mammalian cells with a GFP tag

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

MUTED (BLOC1S5 Human)

Tags

cycle 3 GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2021

Availability

Academic Institutions and Nonprofits only

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p426_Cas9_gRNA-CAN1y

Plasmid

#87391

Purpose

All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting CAN1y sequence GATACGTTCTCTATGGAGGA in yeast chromosome 6.

Depositor

Aindrila Mukhopadhyay

Article

Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

Insert

Human Optimized S. pyogenes Cas9 and guide RNA targeting CAN1y

Use

CRISPR

Promoter

ADH1, pTyrosine

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-OB-Linker-eGFP

Plasmid

#176068

Purpose

EGFP fused to the C-terminus of an OB domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

OB

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pSin-GFP-Fg

Plasmid

#174307

Purpose

Lentiviral expression of GFP

Depositor

Bruce Beutler

Article

Yue et al Science. 2021 May 14;372(6543). pii: 372

Insert

GFP (eGFP Aequorea victoria)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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MiniCoopR mitfa:KIT L576P

Plasmid

#118848

Purpose

Expresses human KIT L576P mutant and zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

KIT L576P (KIT Zebrafish, Human)

Use

Zebrafish expression, tol4

Mutation

KIT L576P

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-iGECI

Plasmid

#160423

Purpose

AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI

Depositor

Vladislav Verkhusha

Article

Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

Insert

iGECI

Use

AAV

Expression

Mammalian

Promoter

CaMKII

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6B CARD8 UPA-CARD-mCherry R464E

Plasmid

#164013

Purpose

Mammalian expression vector encoding CARD8 UPA-CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

mCherry

Expression

Mammalian

Mutation

R464E

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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plenti_DCLK1 G533N

Plasmid

#163627

Purpose

Expresses DCLK1 G533N in E. coli

Depositor

Kenneth Westover

Article

Liu et al Cell Chem Biol. 2020 Oct 15;27(10):1229-

Insert

DCLK1 (DCLK1 Human)

Use

Lentiviral

Tags

V5

Mutation

G33N

Promoter

sv40

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-Rabin8

Plasmid

#118070

Purpose

Expresses Flag-tagged human Rabin8 in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

human Rabin8 (RAB3IP Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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