We narrowed to 18,595 results for: MUT

1. pRK5-myc-Miro2 S430N

   Plasmid

   #47899

   Purpose

   Expresses myc tagged Miro2 S430N dominant negative mutant

   Depositor

   Pontus Aspenström

   Article

   Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

   Insert

   Miro2 S430N (RHOT2 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   S430N, dominant negative mutant

   Promoter

   CMV

   Available Since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pJWK_VD_01

   Plasmid

   #158102

   Purpose

   5'UTR stem-loop luciferase reporter: 0kcal/mol

   Depositor

   Ted Dawson

   Article

   Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

   Insert

   5'UTR synthetic stem-loop 0kcal/mol
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF5/FRT/DEST-3xHA/mGli2 Pc-gE

   Plasmid

   #51256

   Purpose

   Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Glu

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli2 (Gli2 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV

   Expression

   Mammalian

   Mutation

   amino acids 216,220,224,230,248 mutated to Glu

   Promoter

   EF1a

   Available Since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pYPQ239-RR

   Plasmid

   #108862

   Purpose

   FnCpf1 Gateway entry plasmid with N623R and K687R mutations

   Depositor

   Yiping Qi

   Article

   Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

   Insert

   FnCpf1-RR (FnCpf1 with N623R and K687R mutations)
   

   Use

   CRISPR; Gateway compatible cpf1 entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   N623R and K687R, Cpf1 is rice codon optimized

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP TEM4 R130D

   Plasmid

   #58895

   Purpose

   Expresses GFP Tagged full length TEM4 protein with R130D mutation. Please see notes below for information regarding the L722F mutation in TEM4.

   Depositor

   Channing Der

   Article

   Mitin et al PLoS One. 2012;7(7):e41876. doi: 10.1371

   Insert

   TEM4 (ARHGEF17 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   R130D, L722F

   Promoter

   CMV

   Available Since

   Sept. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)_Granulin 2-no linker

   Plasmid

   #176921

   Purpose

   express Granulin 2 without linker 3 in mammalian cells

   Depositor

   Thomas Kukar

   Article

   Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

   Insert

   Granulin 2 (GRN Human)
   

   Tags

   Twin-Strep and FLAG tags encoded after Signal Pep…

   Expression

   Mammalian

   Available Since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)_Granulin 1+linker2

   Plasmid

   #176918

   Purpose

   express Granulin 1 with linker 2 in mammalian cells

   Depositor

   Thomas Kukar

   Article

   Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

   Insert

   Granulin 1+linker2 (GRN Human)
   

   Tags

   Twin-Strep and FLAG tags encoded after Signal Pep…

   Expression

   Mammalian

   Available Since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. psicheck-2-LPPRC-mod

   Plasmid

   #48168

   Purpose

   contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading frame element

   Depositor

   Vamsi Mootha

   Article

   Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

   Insert

   LRPPRC leucine rich pentatricopeptide repeat containing (LRPPRC Human)
   

   Use

   Luciferase

   Mutation

   A/T mutation in the ATG of the uORF

   Available Since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pEGFP-LC3-K51A

   Plasmid

   #155265

   Purpose

   Mammalian expression of rat LC3 fused to EGFP

   Depositor

   David Rubinsztein

   Article

   Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Tags

   pEGFP

   Expression

   Mammalian

   Mutation

   Lysine 51 to Alanine

   Available Since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-LC3-R70A

    Plasmid

    #155264

    Purpose

    Mammalian expression of rat LC3 fused to EGFP

    Depositor

    David Rubinsztein

    Article

    Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Tags

    pEGFP

    Expression

    Mammalian

    Mutation

    Arginine 70 to Alanine

    Available Since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGB 35S:dCas9:Tnos (GB1191)

    Plasmid

    #68223

    Purpose

    Transcriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    dCas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed; human codon optimis…

    Promoter

    35S

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLgw EcoDam-V5-SRF

    Plasmid

    #98602

    Purpose

    Mammalian DamID lentiviral vector forSRF with Dam-V5 using Gateway cloning

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    SRF (Srf Mouse)
    

    Use

    Lentiviral; Damid

    Tags

    Dam (DNA adenine methyltransferase) and V5

    Expression

    Mammalian

    Promoter

    Heat Shock Minimal Promoter

    Available Since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFUSEss-CHIg-mG1_Arg322Lys

    Plasmid

    #105849

    Purpose

    Expression plasmid coding for mutated heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Arg322Lys (EU numbering).

    Depositor

    Joanna Bereta

    Article

    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

    Insert

    IgG1 heavy chain (Ighg1 Mouse)
    

    Expression

    Mammalian

    Mutation

    Arg322Lys in constant region of IgG1 heavy chain

    Promoter

    hEF1-HTLV

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-XRN1__PRKCE

    Plasmid

    #116846

    Purpose

    Lentiviral expression of XRN1__PRKCE fusion

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    XRN1__PRKCE
    

    Use

    Lentiviral

    Available Since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCEP4-FLAG-CCR5-VN

    Plasmid

    #98963

    Purpose

    FLAG-tagged human CCR5 fused to N-terminus of split Venus

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CCR5 (CCR5 Human)
    

    Tags

    FLAG (dykdddd) epitope tag, Signal/leader sequenc…

    Expression

    Mammalian

    Mutation

    Full-length, wildtype

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV9-hSyn iCyt_BrEt_SnFR_PM

    Plasmid

    #177757

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

    Insert

    pAAV9-hSyn iCyt_BrEt_SnFR_PM
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+)_Granulin 4+linker5

    Plasmid

    #176924

    Purpose

    express Granulin 4 with linker 5 in mammalian cells

    Depositor

    Thomas Kukar

    Article

    Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

    Insert

    Granulin 4+linker5 (GRN Human)
    

    Tags

    Twin-Strep and FLAG tags encoded after Signal Pep…

    Expression

    Mammalian

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. human TLNRD1-F250D pET151

    Plasmid

    #159385

    Purpose

    Expresses human TLNRD1 F250D mutant in bacterial cells

    Depositor

    Ben Goult

    Article

    Cowell et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    Talin Rod Domain Containing Protein 1 F250D mutant (TLNRD1 Human)
    

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Changed Phenylalanine 250 to Aspartic Acid (F250D)

    Promoter

    T7

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEF5/FRT/DEST-3xHA/mGli2 P1-4A

    Plasmid

    #51252

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 789,805,817,848 mutated to Ala

    Promoter

    EF1a

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pHAGE-NTRK3__RBPMS

    Plasmid

    #116829

    Purpose

    Lentiviral expression of NTRK3__RBPMS fusion

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    NTRK3__RBPMS
    

    Use

    Lentiviral

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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