We narrowed to 166,390 results for: addgene
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Plasmid#133233PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with NoP (pOGG113), sfGFP (pOGG037) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0380
Plasmid#133230PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter Rlv3841pnifH (pOGG082), mCherry (EC15071) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shMki67
Plasmid#160958PurposeMki67 shRNA in pMKO.1 retroviral vectorDepositorAvailable SinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
tet pLKO.1-shCSE1L v1 puro
Plasmid#192345PurposeLentiviral expression vector for an inducible shCSE1L v1DepositorInsertshCSE1L v1 (CSE1L Human)
UseLentiviralAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
tet pLKO.1-shCSE1L v2 puro
Plasmid#192346PurposeLentiviral expression vector for an inducible shCSE1L v2)DepositorInsertshCSE1L v2 (CSE1L Human)
UseLentiviralAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAG-RORb2
Plasmid#73999PurposeOverexpression of RORb2DepositorAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_GSK3B
Plasmid#111687PurposeMAC-tagged gene expressionDepositorInsertGSK3B (GSK3B Human)
ExpressionMammalianAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pyc128m
Plasmid#158778Purposesoluble GCaMP expressionDepositorInsertGCaMP6m
UseAAVAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
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(517) SB KRAB-SadCas9 U6-gStop
Plasmid#163023PurposeSleeping Beauty TET-On expression of CRISPRi with gSTOP gRNADepositorInsertMammalian codon-optimized SadCas9
UseTransposonTagsKRAB and myc NLSExpressionMammalianPromoterTet-OnAvailable SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
BirA_BVES untagged
Plasmid#196581Purposeexpresses BirADepositorInsertBirA_Ecoli
UseBaculovirus expressionAvailable SinceJune 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPLN-hFluc-DM-eGFP-KDEL
Plasmid#177728PurposeDM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention and fused to eGFP at the C-terminusDepositorInserthFlucDM
TagsKDEL, Prolactin Signal Sequence, and eGFPExpressionBacterial and MammalianMutationR188Q, R261Q (Destabilized Mutant)Available SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
sgRNA1_NANOG
Plasmid#64153PurposePhotoactivatable transcription system. Lentiviral expression of NANOG sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassetteDepositorAvailable SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-iPuro-L3 #BGV205
Plasmid#48987PurposeSupplies the selection marker (puromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInsertpuromycin resistance
UseGateway cloning vectorTagsIRES (weak)PromoterNAAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pCRISPR_RAF1_94
Plasmid#111826PurposeKnockout Raf1DepositorInsertgRNA targeting RAF1 94-114 (RAF1 Human)
UseCRISPRTagsmCherryExpressionBacterial and MammalianPromoterU6Available SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 mir-1-1 reporter hsa-let-7a-1
Plasmid#46670DepositorInserthsa-let-7a-1 (MIRLET7A1 Human)
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianPromoterCMVAvailable SinceJuly 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
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ADARB1_pCSdest
Plasmid#53939DepositorAvailable SinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pgRNA_hMECP2_promoter_No4
Plasmid#194899PurposeLentiviral construct with mCherry and Puro cassettes to express sgRNA-4 targeting the promoter of human MECP2DepositorInsertsgRNA targeting human MECP2 promoter No.4
UseLentiviralExpressionMammalianPromoterU6Available SinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only