We narrowed to 10,802 results for: T7

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MZB218

Plasmid

#221769

Purpose

Bacterial expression of human Arf4 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

Arf4 (ARF4 Human)

Tags

CPD-10xHis

Expression

Bacterial

Mutation

Q71L

Promoter

T7 Promoter

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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MZB219

Plasmid

#221770

Purpose

Bacterial expression of human Arf5 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

Arf5 (ARF5 Human)

Tags

CPD-10xHis

Expression

Bacterial

Mutation

Q71L

Promoter

T7 Promoter

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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MZB220

Plasmid

#221771

Purpose

Bacterial expression of human Arl1 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

Arl1 (ARL1 Human)

Tags

CPD

Expression

Bacterial

Mutation

Q71L

Promoter

T7 Promoter

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-1:ChrA-C63S-C66S

Plasmid

#225082

Purpose

Expresses precursor peptide ChrA-C63S/C66S in E.coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

Insert

ChrA-C63S/C66S

Tags

His6

Expression

Bacterial

Mutation

C63S/C66S

Promoter

T7

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pVF18

Plasmid

#229456

Purpose

Expresses IraD (aa17-130) with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (aa 17-130 only)

Tags

6xHis

Expression

Bacterial

Mutation

aa 17-130 only

Promoter

T7

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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CLIPf-KIF5A(Δ954-1027)-SNAPf-HisTag-FLAG

Plasmid

#200792

Purpose

Mammalian expression of CLIPf-KIF5A(Δ954-1027)-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

KIF5A (Kif5a Mouse)

Tags

CLIPf and SNAPf, HisTag, FLAG

Expression

Mammalian

Mutation

Δ954-1027

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG

Plasmid

#200798

Purpose

Mammalian expression of CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

KIF5A (Kif5a Mouse)

Tags

CLIPf and SNAPf, HisTag, FLAG

Expression

Mammalian

Mutation

411-680

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAM59

Plasmid

#227655

Purpose

pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, Δ78–81)

Depositor

Andreas Martin

Article

Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-

Inserts

Rpn11 (RPN11 Budding Yeast)
Rpn8 (RPN8 Budding Yeast)

Tags

H6-PreScission and PreScission-Strep

Expression

Bacterial

Mutation

1-179 and amino acids 2-239, Δ78–81

Promoter

T7

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET-Cas9-FCPF

Plasmid

#220132

Purpose

Expresses Cas9-FCPF in E.coli

Depositor

Xinlai Cheng

Article

Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

Insert

Cas9-FCPF (cas9 S. pyogenes)

Tags

His and NLS

Expression

Bacterial

Promoter

T7

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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iNicSnFR12

Plasmid

#227142

Purpose

Genetically encoded fluorescent biosensor for nicotine detection

Depositor

Henry Lester

Article

Haloi et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Insert

iNicSnFR12

Tags

6xHis-HA and Myc

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAM94

Plasmid

#226686

Purpose

GGG-TitinV13PV15P-23-K-9-C-25-intein-CBD

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Insert

titin (TTN Human)

Tags

GyrA inteine- Chitin Binding domain and cyclin B

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAM99

Plasmid

#226691

Purpose

TitinV15P-23-K-9-C-1

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Insert

titin (TTN Human)

Tags

GyrA inteine- Chitin Binding domain and cyclin B

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAM97

Plasmid

#226689

Purpose

GGG-Titinv15P-23-K-9-K-9-K-8-C-25

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Insert

titin (TTN Human)

Tags

GyrA inteine- Chitin Binding domain and cyclin B

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAM104

Plasmid

#226696

Purpose

GGG-TitinV15P-43-K-11-intein-CBD

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Insert

titin (TTN Human)

Tags

GyrA inteine- Chitin Binding domain and cyclin B

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAM100

Plasmid

#226692

Purpose

TitinV15P-21-C-2-K-1

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Insert

titin (TTN Human)

Tags

GyrA inteine- Chitin Binding domain and cyclin B

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAM98

Plasmid

#226690

Purpose

TitinV15P-23-K-9-C-15

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Insert

titin (TTN Human)

Tags

GyrA inteine- Chitin Binding domain and cyclin B

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAM105

Plasmid

#226697

Purpose

GGG-TitinV15P-23-K-19-K-11-intein-CBD

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Insert

titin (TTN Human)

Tags

GyrA inteine- Chitin Binding domain and cyclin B

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAM95

Plasmid

#226687

Purpose

GGG-TitinV15P-43-K-8-C-25-intein-CBD

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Insert

titin (TTN Human)

Tags

GyrA inteine- Chitin Binding domain and cyclin B

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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WDV

Plasmid

#226983

Purpose

Expresses the HUH endonuclease WDV, used as a no ligand control for DNA tension sensor experiments

Depositor

Wendy Gordon

Article

Pawlak et al Nat Commun. 2023 Apr 28;14(1):2468. doi:

Insert

WDV (repA )

Tags

6xHis and SUMO

Expression

Bacterial

Promoter

T7

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pJB65

Plasmid

#226319

Purpose

E. coli protein expression for protein purification

Depositor

David Savage

Article

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

6xHis- (VTG to AAA MR1-6)-strep

Tags

6xHis and Strep

Expression

Bacterial

Mutation

N-terminal domain deleted, C-terminal domain dele…

Promoter

T7

Available Since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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