We narrowed to 16,623 results for: puromycin

1. XLone-Puro Cas13d-eGFP U6 BbsI

   Plasmid

   #155184

   Purpose

   PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and U6 gRNA backbone

   Depositor

   Xiaoping Bao

   Article

   Chang et al Biomaterials. 2022 May 6;285:121569. doi

   Type

   Empty backbone

   Use

   Unspecified; Piggybac transposon

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLV[Exp]-Puro-EF-1α>{H2B-RFP}

   Plasmid

   #201121

   Purpose

   RFP expression in cells under EF-1α promoter; cytoplasmic and histone-bound, nuclear

   Depositor

   Ori Bar-Nur

   Article

   Zvick et al Stem Cell Reports. 2022 Sep 13;17(9):194

   Insert

   H2B-RFP
   

   Use

   Lentiviral

   Mutation

   No

   Available Since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LENTICRISPR-UPP1_sgRNA1

   Plasmid

   #201634

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

   Insert

   UPP1 (UPP1 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLKO.1 shRUNX1 puro

   Plasmid

   #45816

   Depositor

   Kevin Janes

   Article

   Wang et al Proc Natl Acad Sci U S A. 2011 Oct 4;108

   Insert

   Runx1 (RUNX1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TFORF1193

   Plasmid

   #143479

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF574 (ZNF574 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PDG459 V3

   Plasmid

   #226958

   Purpose

   CBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1/Puro-CAG-ASAP2s

   Plasmid

   #101274

   Purpose

   Genetically encoded voltage indicator ASAP2s expressed under strong mammalian promoter (CAG)

   Depositor

   Francois St-Pierre

   Article

   Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

   Insert

   ASAP2s
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Human USP7-3X FLAG

   Plasmid

   #225334

   Purpose

   Lentiviral expression of human USP7 with 3x FLAG tag in mammalian cells

   Depositor

   Huda Zoghbi

   Article

   Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

   Insert

   USP7 (USP7 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLEX-uORF-HA-birA*-N-Ras(Q61K)-IRES-Puro

   Plasmid

   #120564

   Purpose

   Expresses HA-birA*-N-Ras Q61K fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   Q61K

   Promoter

   CMV

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLJM1-EGFP-GRAM-H

    Plasmid

    #211703

    Purpose

    Lentiviral expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187L mutation (pLJM1-EGFP-GRAM1b G187L)

    Depositor

    Yasunori Saheki

    Article

    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

    Insert

    EGFP-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Changed Glycine 187 to Leucine

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLEX-uORF-HA-birA*-N-Ras(Q61K/T35A)-IRES-Puro

    Plasmid

    #120565

    Purpose

    Expresses HA-birA*-N-Ras Q61K/T35A fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Q61K, T35A

    Promoter

    CMV

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLEX-uORF-HA-birA*-N-Ras(Q61K/Y40A)-IRES-Puro

    Plasmid

    #120567

    Purpose

    Expresses HA-birA*-N-Ras Q61K/Y40A fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Q61K, Y40A

    Promoter

    CMV

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLEX-uORF-HA-birA*-N-Ras(Q61K/E37A)-IRES-Puro

    Plasmid

    #120566

    Purpose

    Expresses HA-birA*-N-Ras Q61K/E37A fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Q61K, E37A

    Promoter

    CMV

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SPHK1 gRNA (BRDN0001147454)

    Plasmid

    #76813

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SPHK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SPHK1 (SPHK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. STK11 gRNA (BRDN0001146880)

    Plasmid

    #75912

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human STK11

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    STK11 (STK11 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSLQ2821 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI

    Plasmid

    #84257

    Purpose

    Expresses Sp dCas9 and GA-inducible VPR for OR gate/diametric regulation

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sp dCas9-ABI
    GID1-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …

    Expression

    Mammalian

    Promoter

    CAG and PGK

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMGF196

    Plasmid

    #97005

    Purpose

    Expresses LEMD2-mCherry in mammalian cells under a crippled promoter

    Depositor

    Adam Frost, Wesley Sundquist

    Article

    Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    LEMD2 (LEMD2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV delta 5

    Available Since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SPHK1 gRNA (BRDN0001147534)

    Plasmid

    #76814

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SPHK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SPHK1 (SPHK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SPHK2 gRNA (BRDN0001146882)

    Plasmid

    #77096

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SPHK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SPHK2 (SPHK2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SPHK1 gRNA (BRDN0001148403)

    Plasmid

    #76815

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SPHK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SPHK1 (SPHK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge