167,642 results

1. pCK011 p5E exorh

   Plasmid

   #195949

   Purpose

   Gateway p5E vector

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   exorh regulatory elements
   

   Use

   Other

   Available Since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Talin-CS

   Plasmid

   #83377

   Purpose

   Control FRET probe for measuring tension on Talin molecule

   Depositor

   Martin Schwartz

   Article

   Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d

   Insert

   Talin1 (Tln1 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP-F40-tagRFP (tension sensor module)

   Expression

   Mammalian

   Mutation

   Tension sensor module is inserted at C-terminal o…

   Promoter

   CMV

   Available Since

   Oct. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123)

   Plasmid

   #209080

   Purpose

   Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-sfGFP over-expression

   Depositor

   Vikram Khurana

   Article

   Lam et al bioRxiv 2022.11.08.515615

   Inserts

   SNCA (SNCA Human)
   NGN2 (Neurog2 Mouse)
   

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   A53T

   Promoter

   TRE4G

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ223 (dAsCpf1-SRDX)

   Plasmid

   #86209

   Purpose

   Deactivated AsCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmid

   Depositor

   Yiping Qi

   Article

   Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

   Insert

   dAsCpf1
   

   Use

   Gateway compatible cpf1 vectors

   Tags

   NLS and SRDX repressor and NLS

   Expression

   Plant

   Mutation

   D908A

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSW002-Pc-mNeonGreen

   Plasmid

   #205016

   Purpose

   Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mNeonGreen (codon optimized for P. fluorescens)

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   mNeonGreen
   

   Expression

   Bacterial

   Mutation

   mNeonGreen is codon optimized for expression in P…

   Promoter

   Pc

   Available Since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. tetO-PBX1

   Plasmid

   #170699

   Purpose

   doxycycline-inducible overexpression of PBX1

   Depositor

   Paul Burridge

   Article

   Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

   Insert

   PBX1 (PBX1 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet-ON

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-TripleColour-StAR

   Plasmid

   #222695

   Purpose

   Competition assay

   Depositor

   Ulrich Elling

   Article

   Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   TagBFP, miRFP703, dTomato

   Expression

   Mammalian

   Mutation

   PGK, TagBFP, dTomato without BsmBI cutsite

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-mCherry-Rheb

   Plasmid

   #192436

   Purpose

   Encodes mCherry-tagged wild-type Rheb.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-Rheb (RHEB Human)
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Active Wnt7A-V5

   Plasmid

   #43816

   Depositor

   Xi He

   Article

   MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

   Insert

   human WNT7A (WNT7A Human)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGWB505

    Plasmid

    #74847

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. FLAG.PKCepsilon

    Plasmid

    #10795

    Depositor

    Alex Toker

    Article

    Cenni et al Biochem J. 2002 May 1. 363(Pt 3):537-45.

    Insert

    PKC epsilon (PRKCE Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. ActTS-GR

    Plasmid

    #79774

    Purpose

    FRET-based actinin tension sensors

    Depositor

    Tetsuya Kitaguchi

    Article

    Yamashita et al Sci Rep. 2016 Jun 23;6:28535. doi: 10.10

    Insert

    ActTS-GR
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAVU6+27-F30-2xdBroccoli

    Plasmid

    #66842

    Purpose

    Expresses two dimeric Broccoli aptamers in the F30 scaffold in mammalian cells

    Depositor

    Samie Jaffrey

    Article

    Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi

    Insert

    Two dimeric Broccoli aptamers in the F30 scaffold
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1 puro shRNA beta-catenin

    Plasmid

    #18803

    Purpose

    3rd gen lentiviral vector for knocking down beta-catenin gene expression

    Depositor

    Bob Weinberg

    Article

    Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

    Insert

    b-catenin shRNA (CTNNB1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 22, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP (AAV8)

    Viral Prep

    #137148-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP (#137148). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP plasmid DNA. nEF-driven, Cre and Flp-dependent expression of Arch3.3-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    nEF

    Tags

    EYFP (Cre and Flp-dependent)

    Available Since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

16. pCM433kanT

    Plasmid

    #61262

    Purpose

    Sucrose counterselection (sacB) vector for unmarked allelic exchange.

    Depositor

    Mary Lidstrom

    Article

    Puri et al Appl Environ Microbiol. 2014 Dec 29. pii

    Type

    Empty backbone

    Use

    Synthetic Biology; Sucrose counterselection

    Expression

    Bacterial

    Available Since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFH2.10_Gag-MCP-P2A-eGFP

    Plasmid

    #205525

    Purpose

    Express HIV Gag-MCP-P2A-eGFP

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    Gag-MCP
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. CMV-synaptophysin-Lime

    Plasmid

    #201016

    Purpose

    Expresses Lime in synaptic vesicle lumen by fusion to synaptophysin

    Depositor

    Jaime de Juan-Sanz

    Article

    Shen et al ACS Sens. 2023 Aug 25;8(8):3014-3022. do

    Insert

    Synaptophysin-Lime (Syp Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] (AAV5)

    Viral Prep

    #84445-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] (#84445). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] plasmid DNA. CAG-driven, Cre-dependent Jaws-GFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Promoter

    CAG

    Tags

    GFP (Cre-dependent)

    Available Since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

20. pSEVA331Bb

    Plasmid

    #78269

    Purpose

    pSEVA331 with Biobrick multiple cloning site

    Depositor

    Tom Ellis

    Article

    Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

    Type

    Empty backbone

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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