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pEXP5-NT/pLas eGFP

Plasmid

#193630

Purpose

Inducible expression of eGFP by the transcription factor complex LasR-3OC12 homoserine lactone (HSL).

Depositor

Dora Tang

Publication

Gonzales et al ChemSystemsChem 2023

Insert

eGFP

Tags

6xHis

Expression

Bacterial

Promoter

T7 RNAP promoter

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA-ALFA-tag-Gal4-VP16

Plasmid

#199465

Purpose

Expression of the Gal4-ALFA-tag-VP16 synthetic transcription factor containing the ALFA-tag sequence in mammalian cells.

Depositor

Vladislav Verkhusha

Publication

Chernov et al Int J Mol Sci. 2023 Mar 30;24(7):6526. d

Insert

Gal4-ALFA-tag-VP16

Expression

Mammalian

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pMUT2-VtrA-mCherry

Plasmid

#192862

Purpose

pMUT2 based vector for sensing human secondary bile acids in E. coli Nissle 1917

Depositor

Morten Sommer

Publication

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

VtrA-CadC fusion protein and cognate promoter inducible by secondary bile acids

Expression

Bacterial

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On shYAP1-3

Plasmid

#193666

Purpose

Tet inducible knockdown of YAP1

Depositor

William Hahn

Publication

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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EFS eGFP hPGK BSD

Plasmid

#188520

Purpose

Backbone for CRISPR interference (CRISPRi) isogenic PAM testing

Depositor

Isaac Hilton

Publication

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Type

Empty backbone

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pH6-spTorA-mCherry

Plasmid

#169040

Purpose

mCherry preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag in pET28a.

Depositor

Siegfried Musser

Publication

Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

Insert

H6-spTorA-mCherry

Tags

6xHis and spTorA (signal peptide of E. coli TorA)

Expression

Bacterial

Promoter

T7

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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L2_UBE2:mT-N7-CsA

Plasmid

#136133

Purpose

Plasmid to transform Mp with constitutive promoter driving mTurq to the nuclei. HygR

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-35S:HygR p5-MpUBE2:mTurq-N7

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-CTSS

Plasmid

#172415

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Cathepsin S (CTSS Human)

Tags

MAC-tag

Expression

Mammalian

Promoter

CMV promoter

Available since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pMAL728

Plasmid

#169152

Purpose

Encodes OptoQ-AMP6

Depositor

Jose Avalos

Publication

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Mutation

A79Q

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pSEVA221TrxA-AID-T7RNAP

Plasmid

#167976

Purpose

Expresses fusion protein TrxA-AID-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Publication

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

TrxA-AID-T7RNAP

Tags

T7RNAP and TrxA

Expression

Bacterial

Promoter

Ptet

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-HA-GlpG

Plasmid

#155135

Purpose

Expresses N-terminally GFP- and HA-tagged E. coli GlpG from pGEX 4P-1

Depositor

Sinisa Urban

Publication

Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

Insert

GlpG (glpG Escherichia coli)

Tags

GST and HA

Expression

Bacterial

Promoter

tac

Available since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX307-CTSL-G418

Plasmid

#158468

Purpose

Constitutive lentiviral expression of human CTSL.

Depositor

Alejandro Chavez, Sho Iketani

Insert

CTSL (CTSL Human)

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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TMPRSS2 g4 + Cas9

Plasmid

#153018

Purpose

A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

Depositor

Jason Sheltzer

Insert

Cas9 + TMPRSS2 gRNA

Use

CRISPR and Lentiviral

Tags

TagBFP2

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pTNT-pT7-tetR-mCherry

Plasmid

#140870

Purpose

T7 promoter driven synthesis of TetR-mCherry fusion protein

Depositor

Neal Devaraj

Publication

Niederholtmeyer et al Nat Commun. 2018 Nov 28;9(1):5027. doi:

Insert

TetR-mCherry expressed with T7 promoter

Use

Synthetic Biology

Tags

mCherry

Expression

Bacterial

Promoter

T7

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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JB40

Plasmid

#128901

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Publication

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rab5ASN-BirA*-HA-MAO (RAB5A Human)

Expression

Mammalian

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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JB25

Plasmid

#128921

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Publication

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rab33BTN-BirA*-HA-MAO (RAB33B Human)

Expression

Mammalian

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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JB4

Plasmid

#128903

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Publication

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rab6ATN-BirA*-HA-MAO (RAB6A Human)

Expression

Mammalian

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCR2.1 GFR alpha 2

Plasmid

#119899

Purpose

Mouse GFR alpha 2 In situ hybridization probe

Depositor

David Ginty, Wenqin Luo

Publication

Luo et al Neuron. 2009 Dec 24;64(6):841-56. doi: 1

Insert

GDNF family receptor alpha-2 (Gfra2 Mouse)

Use

In situ hybridization probe

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pOGG202

Plasmid

#115503

Purpose

Clone of a level 1 unit which encodes IPTG-inducible sfGFP

Depositor

Philip Poole

Publication

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

pL1M-F1-plac pOGG031, sfGFP pOGG037, T-pharma pOGG003

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pET23a-MmUBC

Plasmid

#69555

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Publication

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBC (Ubc Mouse)

Expression

Bacterial

Promoter

T7

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only