We narrowed to 65,842 results for: cell

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pGGD_MiniTurboID-YFP

Plasmid

#222434

Purpose

Golden Gate / Green Gate module for adding MiniTurboID and YFP as C-terminus of target protein.

Depositor

Dominique Bergmann

Article

Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.

Insert

MiniTurboID (BirA mutant)

Use

Golden gate / green gate compatible cloning vector

Tags

Linker and YFP

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRV.GFP FOXP3

Plasmid

#13250

Depositor

Anjana Rao

Article

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Insert

FOXP3 (FOXP3 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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MSCV-Ccdc134-IRES-BFP

Plasmid

#117266

Purpose

Retroviral overexpression of Ccdc134

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Insert

Ccdc134 (Ccdc134 )

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pET-NlaCascade-NLS-6xHis

Plasmid

#180213

Purpose

Expressing Neisseria lactamica Type I-C Cascade subunits (Cas5-Cas8-Cas11-Cas7) with NLS and His tag on the C terminus of Cas7 for protein purification in E.Coli

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

NlaCas5-Cas8-Cas7

Tags

6XHis and NLS

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-Kif5c

Plasmid

#71853

Purpose

mammalian expression of GFP-Kif5c

Depositor

Michelle Peckham

Article

Dunn et al J Cell Sci. 2008 Apr 1;121(Pt 7):1085-95

Insert

Kif5c (Kif5c Rat)

Tags

EGFP

Expression

Mammalian

Mutation

Kif5c starts at AA3

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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NET5s pEGFP-N2 (1154)

Plasmid

#62034

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

Insert

NET5s (TMEM201 Human)

Tags

EGFP

Expression

Mammalian

Mutation

short form

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-H-Ras_V12

Plasmid

#39504

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 (HRAS Human)

Expression

Mammalian

Mutation

G12V

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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TOMM20-mTurquoise-Rac1-G12V-deltaCaaX

Plasmid

#176124

Purpose

Constitutively active, mitochondrial localized Rac1

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

RAC1 (RAC1 Human)

Expression

Mammalian

Mutation

G12V

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-p65AD-Hsp70

Plasmid

#62914

Purpose

Creating p65AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-p65AD-Hsp70

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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pLL5.0 E-cadherin shRNA/mE-cadherin-mCherry

Plasmid

#101282

Purpose

Lentiviral expression of shRNA and mouse E-cadherin -mCherry

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

E-cadherin

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Available Since

Jan. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-NRCH-PE2max-BSD

Plasmid

#191103

Purpose

Lentiviral expression plasmid of NRCH-PEmax prime editor with P2A-BSD marker

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

NRCH-PEmax

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

Changed R221K, N394K, H840A from SpCas9-NRCH

Promoter

EF-1a

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-GBP

Plasmid

#67650

Purpose

Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pLL5.0 E-cadherin shRNA/Ecad-tdTomato

Plasmid

#101278

Purpose

Lentiviral expression of shRNA targeting Ecad and Ecad-tdtomato

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

E-cadherin

Use

Lentiviral

Tags

tdtomato

Expression

Mammalian

Available Since

Jan. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-hU6-enhanced_gRNA-BFP-P2A-PuroR

Plasmid

#230935

Purpose

gRNA cloning vector encoding BFP and puromycin resistance

Depositor

Charles Gersbach

Article

Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

TagBFP

Promoter

hU6

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(0)-T2A-dVP16AD-Hsp70

Plasmid

#62905

Purpose

Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 0

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-dVP16AD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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T-STOP sgRNA-1

Plasmid

#83346

Purpose

The sgRNA at the stop codon of human Brachyury(T) locus

Depositor

James Thomson

Article

Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

Insert

T-STOP sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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3XFlag pCMV5-TOPO TAZ WT

Plasmid

#24809

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Tags

3XFlag

Expression

Mammalian

Available Since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pBS-KS-attB2-SA(1)-T2A-dVP16AD-Hsp70

Plasmid

#62908

Purpose

Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-dVP16AD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pMSCV(gfp)U6sgRNA5(BbsI)-PGKpuroBFP

Plasmid

#102798

Purpose

Retrovirus for delivery of one sgRNA (self-targeting) - GFP-targeting control plasmid contains GFP instead of PuroR

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Inserts

sgRNA
EGFP

Use

CRISPR and Retroviral

Expression

Mammalian

Mutation

H231L

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-hCD24-T2A-GFP

Plasmid

#205456

Purpose

lentiviral plasmid for expression of human CD24

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

hCD24 (CD24 Human)

Expression

Mammalian

Promoter

UBC

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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