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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 25,284 results for: crispr

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Showing: 7341 - 7360 of 25284 results

  2. pSmart-EF1a-Nla-cas5

    Plasmid
    #178878
    Purpose
    Expression of human codon-optimized Nlacas5 with C terminal NLS and HA tag
    Depositor
    Yan Zhang
    Publication
    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EF1a-NlaCas5c-bGH polyA
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    178878-plasmid-map-sequence-id-351751

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  3. pSmart-EF1a-Nla-cas7

    Plasmid
    #178879
    Purpose
    Expression of human codon-optimized Nlacas7 with C terminal NLS and HA tag
    Depositor
    Yan Zhang
    Publication
    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EF1a-NlaCas7c-bGH PolyA
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    178879-plasmid-map-sequence-id-351739

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  4. pSmart-EF1a-Nla-cas8

    Plasmid
    #178880
    Purpose
    Expression of human codon-optimized NlaCas8 with N terminal NLS and HA tag
    Depositor
    Yan Zhang
    Publication
    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EF1a-NlaCas8c-bGH PolyA
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    178880-plasmid-map-sequence-id-351750

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  5. pSmart-EF1a-Nla-cas11

    Plasmid
    #178882
    Purpose
    Expression of human codon-optimized NlaCas11 with N terminal NLS and HA tag
    Depositor
    Yan Zhang
    Publication
    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EF1a-NlaCas11c-bGH polyA
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    178882-plasmid-map-sequence-id-351753

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  6. pM378

    Plasmid
    #173174
    Purpose
    Expresses C9-1 gRNA-12xMBS from hU6 promoter
    Depositor
    Hajin Kim
    Publication
    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    C9-1 targeting gRNA-12xMBS
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    Aug. 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    173174-plasmid-map-sequence-id-341543

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  10. TMPRSS2 g3

    Plasmid
    #153017
    Purpose
    A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing mCherry
    Depositor
    Jason Sheltzer
    Insert
    TMPRSS2 gRNA
    Use
    CRISPR and Lentiviral
    Tags
    mCherry
    Available since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    153017-plasmid-map-sequence-id-298697

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  14. dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP

    Plasmid
    #154139
    Purpose
    Expresses the dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP fusion protein for targeted DNA methylation.
    Depositor
    Albert Jeltsch
    Publication
    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9, DNMT3a (catalytic domain), DNMT3l (C-terminal part), tagBFP (DNMT3A, DNMT3L Human, Streptococcus pyogenes)
    Tags
    3x FLAG and tagBFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    154139-plasmid-map-sequence-id-345683

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  17. pCMV-dCpf1-eBE-YE

    Plasmid
    #107689
    Purpose
    Expresses dLbCpf1-eBE-YE in mammalian cells
    Depositor
    Jia Chen
    Publication
    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCpf1-eBE-YE
    Expression
    Mammalian
    Mutation
    APOBEC1(W90Y/R126E), dLbCpf1(D832A/E925A/D1148A)
    Promoter
    CMV
    Available since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    107689-plasmid-map-sequence-id-212121

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  19. PABE-7-NG

    Plasmid
    #138142
    Purpose
    Targeted A to G in rice protoplasts
    Depositor
    Caixia Gao
    Insert
    wtTadA-TadA7.10-nCas9-NG-3XNLS
    Expression
    Plant
    Available since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    138142-plasmid-map-sequence-id-311738

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  20. AW01_pU.CAG.eSpCas9(1.1).rBGpA

    Plasmid
    #199255
    Purpose
    Expression construct encoding the high specificity eSpCas9(1.1) nuclease
    Depositor
    Manuel Goncalves
    Publication
    Chen et al Nucleic Acids Res. 2016 Jun 8. pii: gkw5
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    High specificity eSpCas9(1.1) nuclease
    Use
    CRISPR
    Tags
    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    K848A K1003A R1060A
    Promoter
    CAG promoter
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    199255-plasmid-map-sequence-id-391368

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Showing: 7341 - 7360 of 25284 results