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pTagBFP2-C1-lynN11-FKBP-PI-PLCN187

Plasmid

#139308

Purpose

N-terminal half of split PI-PLC targeted to plasma membrane

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

LYN(1-11):RSANS[GA]4:TagBFP2: FKBP1A(3-108): [GGSA]4GG:L. monocytogenes PI-PLC(1-187)

Expression

Mammalian

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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Opto-ACTU−

Plasmid

#198869

Purpose

Optogenetic recruitment construct to increase inner membrane surface charge

Depositor

Peter Devreotes

Article

Banerjee et al Nat Cell Biol. 2022 Oct;24(10):1499-1515

Insert

Rad17 polyanionic tail (-14)

Use

Synthetic Biology

Tags

CRY2PHR-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLV_ADRB2-mTurquoise2-iLID

Plasmid

#161002

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to ADRB2 for slow diffusion

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

ADRB2-mTurquoise2-iLID

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pEx-insertion-erm

Plasmid

#172210

Purpose

For genetic insertion in erythromycin-sensitive Prevotella copri strains

Depositor

Till Strowig

Article

Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

Type

Empty backbone

Use

Genetic insertion in prevotella copri

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_C_LMTK3

Plasmid

#187773

Purpose

MAC-tagged gene expression of human LMTK3

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

LMTK3 (LMTK3 Human)

Expression

Mammalian

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_C_LTK

Plasmid

#187762

Purpose

MAC-tagged gene expression of human LTK

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

LTK (LTK Human)

Expression

Mammalian

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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5HT6-YFP-PIPK

Plasmid

#96810

Purpose

cilia-membrane-targeted phosphatidylinositol 4-phosphate 5-kinase

Depositor

Takanari Inoue

Article

Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

Insert

5HT6-YFP-PIPK

Tags

YFP

Expression

Mammalian

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pEx-insertion-erm-T1T2

Plasmid

#172212

Purpose

For genetic insertion in erythromycin-sensitive Prevotella copri strains, with T1-T2 terminator

Depositor

Till Strowig

Article

Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

Type

Empty backbone

Use

Genetic insertion in prevotella copri

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNDGG003

Plasmid

#231316

Purpose

BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Tc-par-ELT4; GG assembly from pOGG010, pOGG012, pOGG014, pOGG042, and pOGG004, TcR

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Type

Empty backbone

Expression

Bacterial

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-MLC2 WT

Plasmid

#195235

Purpose

Mammalian expression of GFP tagged MLC2. Wild-type

Depositor

David Bryant, Keith Mostov

Article

Yu et al EMBO Rep. 2008 Sep;9(9):923-9. doi: 10.1

Insert

MLC2 (MYL2 Human)

Expression

Mammalian

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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