We narrowed to 4,489 results for: 256
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PtetA-PBAD-PLacO3O1 200bp with pBELO_L6-PT3-T4_CAMR_TAN+
Plasmid#225649PurposetetA, araBAD and LacO3O1 promoters in tandem conformation with 200bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistanceDepositorInserttetR/tetA, araBAD and LacO3O1 in tandem conformation
TagsmCherryExpressionBacterialPromotertetA, araBAD and LacO3O1 promotersAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFsynW SYT1-PGM reporter
Plasmid#197278PurposeEncodes for viral expression of Synaptotagmin 1 (SYT1) for use in the RUSH system; visualized with HaloTag. Reporter only (no hook). SYT1 is mutated to disrupt palmitoylation and glycosylation.DepositorInsertSYT1 and HaloTag (Syt1 Rat)
UseLentiviralTagsFLAGMutationT15A, T16A, N24Q, C74A, C75A, C77A, C79A, and C82APromoterhSyn (human synapsin I promoter)Available SinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
mU6-sgCebpb_v2-hu6-sgCebpd_v2
Plasmid#177256PurposeExpresses Cebpb_v2 (mU6), Cebpd_v2(hU6) gRNAs and Cre-recombinaseDepositorInsertsgCebpb_v2/sgCebpd_v2
UseLentiviralPromotermU6/hU6Available SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_DHN_HbDHN1 (pBS0788)
Plasmid#185256PurposeFor the mammalian expression of the Para rubber tree protein DHN_HbDHN1. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertDHN_HbDHN1
ExpressionMammalianAvailable SinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-Hypa-nCas9n-A69
Plasmid#125645Purposemicro-injection of poly-adenylated Hypa-nCas9n RNADepositorInsertHypa-nCas9n
UseRna in vitro transcription vectorMutationN692A, M694A, Q695A, H698A from wild-type Cas9PromoterT3Available SinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEMS1957
Plasmid#82568PurposeHigh copy number plasmid containing hs1218 MiniPromoter insert.DepositorInserthshs1218
UsePleiades promoter project [sic, pleaides plieades]Available SinceSept. 14, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS1953
Plasmid#82567PurposeHigh copy number plasmid containing hs671 MiniPromoter insert.DepositorInserths671
UsePleiades promoter project [sic, pleaides plieades]Available SinceSept. 14, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS1944
Plasmid#82566PurposeHigh copy plasmid containing Ple267 (UGT8 MiniPromoter) insert.DepositorInsertPle267
UsePleiades promoter project [sic, pleaides plieades]Available SinceSept. 14, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
Human Epigenetic Knockout Library
Pooled Library#162256PurposeKnockout library that targets human epigenetic genes.DepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Human SLC SAM Library
Pooled Library#132561PurposeActivation library targeting human SLC proteins.DepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable SinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFsynW SYB2 reporter
Plasmid#197276PurposeEncodes for viral expression of wild type Synaptobrevin 2 (SYB2) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).DepositorInsertSYB2 and HaloTag (Vamp2 Rat)
UseLentiviralTagsFLAGMutationN/APromoterhSyn (human synapsin I promoter)Available SinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
GS2.1/EC
Plasmid#132568PurposesgRNA targeting A. thaliana pds3, regulated by A. thaliana U6 polIII promoter. Cas9 with a C-terminal mApple fusion, egg cell-specific expression. Hygromycin selectable marker (hpt).DepositorInsertegg cell promoter, Cas9-mApple, pds3 sgRNA
UseCRISPR and Synthetic BiologyExpressionPlantPromoterA. thaliana egg cell promoterAvailable SinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEND-184_Cacnes_mCherry
Plasmid#225610PurposeReplicative vector for red fluorescent reporter protein mCherry strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCherry.DepositorInsertmCherry
UseSynthetic BiologyTagsV5-6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-227_Cacnes_mCerulean
Plasmid#225614PurposeReplicative vector for blue fluorescent reporter protein mCerulean strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCerulean.DepositorInsertmCerulean
UseSynthetic BiologyTags6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRubiG-T2A-Cas9
Plasmid#75348PurposeUbiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.DepositorInsertCas9
UseCRISPR and RetroviralTagsGFP (via t2a)ExpressionMammalianPromoterUbiquitinAvailable SinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRubiC-T2A-Cas9
Plasmid#75347PurposeUbiquitin promoter expresses mCherry and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.DepositorInsertCas9
UseCRISPR and RetroviralTagsmCherry (via t2a)ExpressionMammalianPromoterUbiquitinAvailable SinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Puro-CAG-ASAP2s
Plasmid#101274PurposeGenetically encoded voltage indicator ASAP2s expressed under strong mammalian promoter (CAG)DepositorInsertASAP2s
ExpressionMammalianPromoterCAGAvailable SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
YES1 (T348I) in pLX303
Plasmid#42564DepositorAvailable SinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
TFORF0233
Plasmid#142567PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only
