Skip to main content
Addgene
Showing: 721 - 740 of 3138 results

  2. Unc104_K560GFP

    Plasmid
    #129773
    Purpose
    Expresses worm Kinesin-3 Unc104 head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged
    Depositor
    William Hancock
    Insert
    Unc104 (unc-104 Nematode)
    Use
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 361, fused to kinesin-1 starting Al…
    Promoter
    Available since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129773-plasmid-map-sequence-id-263568

    Enlarge

  11. pBridge-tyrosinase.σ1A

    Plasmid
    #197381
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-1 σ1A fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-1 σ1A
    Use
    Tags
    GAL4-DNA binding domain fragment, HA tag, and SV4…
    Expression
    Yeast
    Mutation
    Contains an extra 42 nucleotides encoding 14 resi…
    Promoter
    ADH1 and MET25
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197381-plasmid-map-sequence-id-388878

    Enlarge

  12. pTK331

    Plasmid
    #59572
    Purpose
    TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceI
    Depositor
    Ron Weiss
    Article
    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038
    Insert
    SGP(16) 2xK-turn Kozak EGFP attB2 AscI
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    59572-plasmid-map-sequence-id-92977

    Enlarge

  14. pM-CBE

    Plasmid
    #196293
    Purpose
    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding CBE in place of viral GP
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV M antigenome encoding CBE in place of viral GP
    Use
    CRISPR
    Tags
    3×flag
    Expression
    Plant
    Mutation
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available since
    April 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    196293-plasmid-map-sequence-id-389996

    Enlarge

Showing: 721 - 740 of 3138 results