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pRS415-TEF1pr-NFAPoptim

Plasmid

#221088

Purpose

FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-TEF1pr-CFAPoptim

Plasmid

#221089

Purpose

FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-NFAPorig

Plasmid

#221091

Purpose

FAP insert for N-terminally tagging genes of interest (original FAP + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS413-TEF1pr-NFAPoptim

Plasmid

#221093

Purpose

FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS413-TEF1pr-CFAPoptim

Plasmid

#221094

Purpose

FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS415-TEF1pr-NFAPorig

Plasmid

#221086

Purpose

FAP insert for N-terminally tagging genes of interest (original FAP + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS415-TEF1pr-CFAPorig

Plasmid

#221087

Purpose

FAP insert for C-terminally tagging genes of interest (original FAP + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1a-hfCas13d-pa

Plasmid

#233037

Purpose

To express hfCas13d from a EF1a promoter

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

hfCas13d

Use

AAV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1031 (pME_V5_LEF1_P2A_H2A_mCherry)

Plasmid

#232123

Purpose

A middle entry gateway clone containing V5 tagged human LEF1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

LEF1 (LEF1 Human)

Use

Gateway cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_W208L

Plasmid

#221486

Purpose

NKX2-1 W208L DNA binding mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

W208L

Promoter

EF1a

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_V205F

Plasmid

#221485

Purpose

NKX2-1 V205F DNA binding mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

V205F

Promoter

EF1a

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-GFP

Plasmid

#207018

Purpose

Over-expression of GFP under TEF1 promoter, use auxotrophic marker LEU2(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

GFP

Expression

Yeast

Promoter

pTEF1

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a-hP53DD (TET)

Plasmid

#214083

Purpose

Mammalian expression tetramerization domain (TET) of human TP53

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

hP53DD (TP53 Human)

Expression

Mammalian

Promoter

EF1a

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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EEF1A2 - I438M in pENTR

Plasmid

#216591

Purpose

gateway cloning donor vector containing EEF1A2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

EEF1A2 (EEF1A2 Human)

Use

Gateway entry plasmid

Mutation

I438M

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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EEF1A2 - R382H in pENTR

Plasmid

#216590

Purpose

gateway cloning donor vector containing EEF1A2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

EEF1A2 (EEF1A2 Human)

Use

Gateway entry plasmid

Mutation

R382H

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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EEF1A2 - G305S in pENTR

Plasmid

#216589

Purpose

gateway cloning donor vector containing EEF1A2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

EEF1A2 (EEF1A2 Human)

Use

Gateway entry plasmid

Mutation

G305S

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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EEF1A2 - D252H in pENTR

Plasmid

#216588

Purpose

gateway cloning donor vector containing EEF1A2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

EEF1A2 (EEF1A2 Human)

Use

Gateway entry plasmid

Mutation

D252H

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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EEF1A2 - E122K in pENTR

Plasmid

#216587

Purpose

gateway cloning donor vector containing EEF1A2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

EEF1A2 (EEF1A2 Human)

Use

Gateway entry plasmid

Mutation

E122K

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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EEF1A2 - G70S in pENTR

Plasmid

#216586

Purpose

gateway cloning donor vector containing EEF1A2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

EEF1A2 (EEF1A2 Human)

Use

Gateway entry plasmid

Mutation

G70S

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a-SRRD-HA (in pLEX_307)

Plasmid

#214918

Purpose

constitutive expression of SRRD tagged with HA

Depositor

Ophir Shalem

Article

Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

Insert

SRRD (SRRD Human)

Use

Lentiviral

Tags

HA

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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