Addgene

We narrowed to 15,118 results for: nans

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pDG04093

Plasmid

#196005

Purpose

Nanobody Obs-Nano55 production

Depositor

Dirk Görlich

Insert

H14-SUMO-Cys- ObsNano55-Cys

Use

E.coli expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDG04108

Plasmid

#196010

Purpose

Nanobody Actn-Nano62 production

Depositor

Dirk Görlich

Insert

H14-SUMO-Cys- ActnNano62-Cys

Use

E.coli expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDG04096

Plasmid

#196007

Purpose

Nanobody Obs-Nano57 production

Depositor

Dirk Görlich

Insert

H14-SUMO-Cys- ObsNano57-Cys

Use

E.coli expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDG04109

Plasmid

#196011

Purpose

Nanobody Actn-Nano63 production

Depositor

Dirk Görlich

Insert

H14-SUMO-Cys- ActnNano63-Cys

Use

E.coli expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGFPHYG2

Plasmid

#30173

Depositor

Lalita Ramakrishnan

Article

Cosma et al Infect Immun. 2006 Jun . 74(6):3125-33.

Insert

Mycobacterium Strong Promoter (MSP)

Use

Mycobacteria expression

Tags

GFP

Expression

Mutation

GFPmut3 allele has improved brightness.

Promoter

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

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pYUB1062-GFP

Plasmid

#117695

Purpose

Express recombinant proteins with GFP-His fusion in Mycobacterium smegmatis

Depositor

Elizabeth Fullam

Article

Radhakrishnan et al RSC Adv. 2018 Sep 27;8(58):33087-33095.

Type

Empty backbone

Use

Tags

GFP and His

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pTrcHIS-7D12Gln13TAG

Plasmid

#125264

Purpose

Expresses 7D12 anti-EGFR nanobodies with UAG codon at Gln13 for synthetic amino acid in combination with pEVOL-pAzF

Depositor

Angus Johnston

Article

Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

Insert

Gln13TAG 7D12

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pBiex-1 Myosin VI HMM_SNAP

Plasmid

#82716

Purpose

Expresses Myosin VI HMM with a C-terminal SNAP tag.

Depositor

Sivaraj Sivaramakrishnan

Article

Hariadi et al Nat Nanotechnol. 2015 Aug;10(8):696-700.

Insert

Myosin VI

Use

Tags

6xHis Tag, FLAG purification tag, and SNAP tag

Expression

Bacterial and Insect

Mutation

Promoter

T7 Promoter

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pTrcHis-7D12CTermTAG

Plasmid

#125267

Purpose

Expresses 7D12 anti-EGFR nanobodies with UAG codon at c-terminus for synthetic amino acid in combination with pEVOL-pAzF

Depositor

Angus Johnston

Article

Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

Insert

CTermTAG

Use

Tags

His-Tag

Expression

Mutation

Promoter

Trc

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pLJM1-NtermRGS2-GFP-CIBN

Plasmid

#112258

Purpose

Expresses alpha-helix component of opto-RGS2 in mammalian cells

Depositor

Brian Chow

Article

Hannanta-Anan et al ACS Synth Biol. 2018 Jun 15;7(6):1488-14

Insert

NtermRGS2-GFP-CIBN

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pTrcHis-7D12Asp73TAG

Plasmid

#125266

Purpose

Expresses 7D12 anti-EGFR nanobodies with UAG codon at Asp73 for synthetic amino acid in combination with pEVOL-pAzF

Depositor

Angus Johnston

Article

Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

Insert

7D12 Asp73TAG

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pYFJ16-LplA(W37V) (for E. Coli expression)

Plasmid

#34838

Depositor

Alice Ting

Article

Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

Insert

LplA W37V (lplA E. Coli)

Use

Tags

His Tag

Expression

Bacterial

Mutation

Mutated Trp37 to Val

Promoter

T5 promoter

Available since

May 16, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-InPAkt

Plasmid

#181914

Purpose

Indicator of Phosphoinositides using Akt; genetically encoded FRET-based sensor for monitoring 3-phosphoinositide dynamics in living cells.

Depositor

Jin Zhang

Article

Ananthanarayanan et al Proc Natl Acad Sci U S A. 2005 Oct 18;10

Insert

InPAkt

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pLJM1-CRY2-mCherry-(GGGGS)3-hRGS2box

Plasmid

#112257

Purpose

Expresses RGS component of opto-RGS2 in mammalian cells

Depositor

Brian Chow

Article

Hannanta-Anan et al ACS Synth Biol. 2018 Jun 15;7(6):1488-14

Insert

CRY2-mCherry-(GGGGS)3-hRGS2box

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pSG5-FLAG-mEKLF

Plasmid

#67833

Purpose

Expression of FL-EKLF driven by SV40 promoter

Depositor

James Bieker

Article

Soni et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

EKLF (Klf1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Full-length mEKLF is aa 20-376; amino acid 19 is …

Promoter

SV40

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only
pBW_0065

Plasmid

#102817

Purpose

pVec8 containing Sr22 gene

Depositor

Brande Wulff

Article

Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

Insert

wheat stem rust resistance gene Sr22 (NEWENTRY Synthetic)

Use

Tags

Expression

Plant

Mutation

Promoter

Native

Available since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pBW_0059

Plasmid

#102819

Purpose

pAGM4723 containing Sr35 gene

Depositor

Brande Wulff

Article

Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

Insert

wheat stem rust resistance gene Sr35 (NEWENTRY Synthetic)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Native

Available since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pBW_0141

Plasmid

#102820

Purpose

pVec8 containing Sr45 gene

Depositor

Brande Wulff

Article

Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

Insert

wheat stem rust resistance gene Sr45 (NEWENTRY Synthetic)

Use

Tags

Expression

Plant

Mutation

Promoter

Sr33

Available since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PM-InPAkt

Plasmid

#181915

Purpose

Indicator of Phosphoinositides using Akt; targeted to the plasma membrane.

Depositor

Jin Zhang

Article

Ananthanarayanan et al Proc Natl Acad Sci U S A. 2005 Oct 18;10

Insert

pm-InPAkt

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Contains the following mutations with respect to …

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBW_0001

Plasmid

#102833

Purpose

Binary vector containing Sr22 Schomburgk allele driven by maize ubiquitin promoter, Sr22 terminator

Depositor

Brande Wulff

Article

Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

Insert

Sr22 Schomburgk (NEWENTRY Synthetic)

Use

Tags

Expression

Plant

Mutation

Promoter

Maize ubiquitin

Available since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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