We narrowed to 7,650 results for: ski

1. pCXLE-dCas9VP192-T2A-EGFP

   Plasmid

   #69536

   Purpose

   Episomal plasmid encoding dCas9VP192

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

   Insert

   dCas9-dCas9VP192-EGFP
   

   Use

   CRISPR

   Tags

   EGFP

   Promoter

   CAG

   Available Since

   Nov. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVTU_260_BirA

   Plasmid

   #63682

   Purpose

   Encodes E. coli biotin ligase gene (BirA) for expression in yeast cells

   Depositor

   Aaron Hoskins

   Article

   Rodgers et al RNA. 2015 May;21(5):1031-41. doi: 10.126

   Insert

   BirA
   

   Tags

   6X Histidine and TEV cleavage site

   Expression

   Yeast

   Promoter

   ADH1/yeast

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. TAF3 PHD_pGEX-6P-2

   Plasmid

   #92100

   Purpose

   Expresses GST fusion TAF3 PHD domain protein

   Depositor

   Albert Jeltsch

   Article

   Kungulovski et al Epigenetics Chromatin. 2016 Mar 22;9:11.

   Insert

   TAF3 PHD domain (TAF3 Human)
   

   Tags

   GST Tag

   Expression

   Bacterial

   Promoter

   tac promoter

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEutRwc

   Plasmid

   #216334

   Purpose

   An IPTG-inducible promoter controlling expression of eutR; PEutS, extended controlling expression of sfgfp

   Depositor

   Mark Styczynski

   Article

   McNerney et al ACS Synth Biol. 2020 Feb 21;9(2):402-411

   Inserts

   EutR
   Superfolder green fluorescent protein
   

   Expression

   Bacterial

   Available Since

   Dec. 23, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. env8 reverse J1/13

   Plasmid

   #99831

   Purpose

   GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

   Depositor

   Robert Batey

   Article

   Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

   Insert

   env8 reverse J1/13
   

   Tags

   GFPuv

   Expression

   Bacterial

   Mutation

   J1/13 sequence is reversed

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. rTetR-GFP

   Plasmid

   #183916

   Purpose

   Reverse Tet repressor labeled with GFP; binds tetO sites upon doxycycline addition

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   rTetR-GFP
   

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. DHFR-myc

   Plasmid

   #20214

   Depositor

   Carolyn Bertozzi

   Article

   Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

   Insert

   DHFR (folA E. coli)
   

   Tags

   myc

   Expression

   Mammalian

   Available Since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CMV-Flag-Rat Arc-Arc- Genomic 3'UTR

   Plasmid

   #233053

   Purpose

   To express Flag-tagged Rat Arc from a CMV promoter. The Arc gene contains the Rat Arc native Genomic 3'UTR containing two introns

   Depositor

   Jonathan Ploski

   Insert

   Rat Arc (Arc Rat)
   

   Use

   AAV

   Tags

   Flag

   Available Since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMC-J6-dCas9-10XGP41-J9

   Plasmid

   #202005

   Purpose

   MoClo level 0 dCas9-10XGP41 coding sequence module

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

   Insert

   dCas9-10XGP41
   

   Use

   Synthetic Biology; Moclo cloning vector

   Tags

   GS linkers

   Available Since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCN38-yfp

    Plasmid

    #227637

    Purpose

    Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

    Depositor

    Angelika Grundling

    Article

    Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

    Insert

    yfp
    

    Expression

    Bacterial

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Endoplasmic reticulum-targeted optoPLD

    Plasmid

    #140060

    Purpose

    Encodes CRY2-mCherry-PLD-P2A-CIBN-Sac1(TM) for mammalian expression

    Depositor

    Jeremy Baskin

    Article

    Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Phospholipase D
    

    Tags

    CIBN, CRY2, P2A, Sac1(TM), and mCherry

    Expression

    Mammalian

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA6a-mScarlet-I-mNeonGreen-kanMX (pKBJ007)

    Plasmid

    #173454

    Purpose

    Contains mScarletI-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mScarlet-I-mNeonGreen
    

    Expression

    Yeast

    Promoter

    no promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFA6a-mScarlet-mNeonGreen-kanMX (pKBJ008)

    Plasmid

    #173455

    Purpose

    Contains mScarlet-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mScarlet-mNeonGreen
    

    Expression

    Yeast

    Promoter

    no promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Endosome-targeted optoPLD

    Plasmid

    #140056

    Purpose

    Encodes CRY2-mCherry-PLD-P2A-CIBN-2xFYVE for mammalian expression

    Depositor

    Jeremy Baskin

    Article

    Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Phospholipase D
    

    Tags

    2xFYVE, CIBN, CRY2, P2A, and mCherry

    Expression

    Mammalian

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCellFree_G03

    Plasmid

    #67137

    Purpose

    Gateway destination vector for cell-free expression with N-term 8xHis eGFP tag

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

    Type

    Empty backbone

    Use

    Cell-free expression

    Tags

    EGFP and His

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCellFree_G03 TFEC

    Plasmid

    #67060

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TFEC (TFEC Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pAAV.GFA104.PI.eGFP.WPRE.bGH

    Plasmid

    #100896

    Purpose

    AAV expression of EGFP in astrocytes

    Depositor

    Philip Haydon

    Article

    Ortinski et al Nat Neurosci. 2010 May;13(5):584-91. doi

    Has Service

    AAV5

    Insert

    EGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    GFA104 (GFAP promoter)

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. plvx-AD8

    Plasmid

    #191558

    Purpose

    Inducible expression of HIV-1 Env

    Depositor

    Joseph Sodroski, Alon Herschhorn

    Article

    Herschhorn et al PLoS One. 2011;6(11):e26731. doi: 10.137

    Insert

    HIV-1 AD8 env
    

    Use

    Lentiviral

    Mutation

    None

    Available Since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6-cGAS g2 Cas9-T2A-mCherry

    Plasmid

    #164253

    Purpose

    Transcription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    sgcGAS
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. FRB-myc

    Plasmid

    #20228

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    FRB (MTOR Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    T2098L point mutation

    Available Since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only