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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,675 results for: BLI

Showing: 7401 - 7420 of 9675 results

  1. pUC-ODN-hsRPL3-CterTwStrep

    Plasmid
    #208644
    Purpose
    for long single strand DNA donnor preparation for Twin Strep tag knock-in at C ter of human RPL3. Digest with nt.BspQI and nb.BsrDI to get ssDNA. This plasmid will cause re-edit and fail knock-in.
    Depositor
    Quanfu Ma
    Insert
    Twin Strep tag with RPL3 genome HomoArm (RPL3 Human, Synthetic)
    Use
    CRISPR; Ssdna donnor preparation
    Tags
    Twin Strep
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208644-plasmid-map-sequence-id-418358

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  2. pcDNA3.1-msEGFP1.8

    Plasmid
    #208619
    Purpose
    Mammalian expression of a EGFP variant. Mutations were adopted from (Addgene#135301, PMC7508230), named msEGFP1.8 because N-ter and C-ter features of that in msGFP2 (PMC7508230) not included.
    Depositor
    Quanfu Ma
    Insert
    msEGFP1.8
    Expression
    Mammalian
    Mutation
    (compared with pEGFP-C1, protein level) S30R, Y39…
    Promoter
    CMV
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208619-plasmid-map-sequence-id-418315

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  3. GOSR2(G144W)-mCherry

    Plasmid
    #209887
    Purpose
    Expresses human GOSR2 (GS27) G144W-mutant fused to mChery in mammalian cells
    Depositor
    Geert van den Bogaart
    Insert
    GOSR2 (GOSR2 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Changed glycine 144 to tryptophan; this mutation …
    Promoter
    CMV
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    209887-plasmid-map-sequence-id-413669

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  5. pVRC8400-SARS-CoV-2-NTD-BA.4/5-AVI

    Plasmid
    #192113
    Purpose
    Expresses SARS-CoV-2 NTD protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Insert
    SARS-CoV-2-NTD-BA.4/5 (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    T19I, L24S, P25-, P26-, A27-, H69-, V70-, G142D, …
    Available Since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    192113-plasmid-map-sequence-id-374987

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  6. human KANK2-A670V Ankyrin Repeats pET151

    Plasmid
    #164055
    Purpose
    Expresses human KANK2 Ank domain - A670V mutant in bacterial cells
    Depositor
    Ben Goult
    Insert
    Kidney Ankyrin Repeat-Containing Protein 2 A670V mutant (KANK2 Human)
    Tags
    His-tag, TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    164055-plasmid-map-sequence-id-327673

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  7. mouse KANK3 Ankyrin Repeats pET151

    Plasmid
    #164056
    Purpose
    Expresses mouse KANK3 Ank domain in bacterial cells
    Depositor
    Ben Goult
    Insert
    Kidney Ankyrin Repeat-Containing Protein 3 (Kank3 Mouse)
    Tags
    His-tag, TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    164056-plasmid-map-sequence-id-327670

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  8. mouse KANK4 Ankyrin Repeats pET151

    Plasmid
    #164057
    Purpose
    Expresses mouse KANK4 Ank domain in bacterial cells
    Depositor
    Ben Goult
    Insert
    Kidney Ankyrin Repeat-Containing Protein 4 (Kank4 Mouse)
    Tags
    His-tag, TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    164057-plasmid-map-sequence-id-327675

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  10. pCDZ2FE_Champ1_1-812_2PA

    Plasmid
    #105676
    Purpose
    Expresses Flag-tagged full-length human Champ1 protein with mutations abolishing Rev7 binding
    Depositor
    Thanos Halazonetis
    Insert
    Champ1 (CHAMP1 Human)
    Tags
    2X Flag tag
    Expression
    Mammalian
    Mutation
    Mutations: P336A, P341A
    Available Since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105676-plasmid-map-sequence-id-203181

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  11. pT5T_Rev7_1-211_SA112_KVK_YAK

    Plasmid
    #105630
    Purpose
    Expresses mouse monomeric Rev7 protein with mutations abolishing binding to Champ1 and Rev3
    Depositor
    Thanos Halazonetis
    Insert
    Rev7 (Mad2l2 Mouse)
    Expression
    Bacterial
    Mutation
    Mutations: F11S, G12A, I41Y, V132K, C133V, A135K,…
    Promoter
    T7
    Available Since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105630-plasmid-map-sequence-id-203820

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  12. MS2_SC2_S_B.1.640

    Plasmid
    #180228
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the B.1.640 SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Expression
    Bacterial
    Mutation
    B.1.640 (Amino Acid 319-869)
    Promoter
    T7
    Available Since
    Jan. 28, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    180228-plasmid-map-sequence-id-352513

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  13. CD44U-c010

    Plasmid
    #175453
    Purpose
    Protein expression/secretion in mammalian cells. CD44 splice variant 10 (exons 2-5, 11, 15-16), A20-E649.
    Depositor
    Opher Gileadi
    Insert
    CD44 (CD44 Human)
    Tags
    His6
    Expression
    Mammalian
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175453-plasmid-map-sequence-id-353218

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  14. MS2_SC2_S_C.1.2

    Plasmid
    #177367
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the C1.2 SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Expression
    Bacterial
    Mutation
    C1.2 (Amino Acid 319-869)
    Promoter
    T7
    Available Since
    Jan. 20, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    177367-plasmid-map-sequence-id-347828

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  15. MS2_SC2_S_Mu

    Plasmid
    #177366
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Mu SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Expression
    Bacterial
    Mutation
    Mu (Amino Acid 319-869)
    Promoter
    T7
    Available Since
    Jan. 20, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    177366-plasmid-map-sequence-id-347825

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  16. MS2_SC2_S_Kappa

    Plasmid
    #175953
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Kappa SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Expression
    Bacterial
    Mutation
    Kappa (Amino Acid 319-869)
    Promoter
    T7
    Available Since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    175953-plasmid-map-sequence-id-343945

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  17. MS2_SC2_S_Eta

    Plasmid
    #175954
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Eta SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Expression
    Bacterial
    Mutation
    Eta (Amino Acid 319-869)
    Promoter
    T7
    Available Since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    175954-plasmid-map-sequence-id-343942

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  18. MS2_SC2_S_Gamma

    Plasmid
    #175047
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Gamma SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Expression
    Bacterial
    Mutation
    Gamma (Amino Acid 319-869)
    Promoter
    T7
    Available Since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    175047-plasmid-map-sequence-id-343946

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  19. MS2_SC2_S_Theta

    Plasmid
    #175951
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Theta SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Expression
    Bacterial
    Mutation
    Theta (Amino Acid 319-869)
    Promoter
    T7
    Available Since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    175951-plasmid-map-sequence-id-343941

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  20. MS2_SC2_S_Zeta

    Plasmid
    #175952
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Zeta SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Expression
    Bacterial
    Mutation
    Zeta (Amino Acid 319-869)
    Promoter
    T7
    Available Since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    175952-plasmid-map-sequence-id-343947

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Showing: 7401 - 7420 of 9675 results