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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 26,412 results for: Cas9

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Showing: 7401 - 7420 of 26412 results

  8. dSpCas9-VPR

    Plasmid
    #205247
    Purpose
    In vitro transcription plasmid for production of dSpCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #63798.
    Depositor
    Rasmus Bak
    Publication
    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d
    Insert
    dSpCas9-VPR
    Use
    CRISPR
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    205247-plasmid-map-sequence-id-408440

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  9. pZ8-P_dCas9

    Plasmid
    #74063
    Purpose
    pZ8-1 plasmid carrying dcas9 driven by the propionate-inducible prpD2 promoter (PprpD2), KanR
    Depositor
    Timothy Lu
    Publication
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    dcas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Propionate inducible promoter (prp)
    Available since
    April 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    74063-plasmid-map-sequence-id-141970

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  12. pmiCas9

    Plasmid
    #162758
    Purpose
    express miCas9
    Depositor
    Eugene Chen
    Publication
    Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    miCas9
    Use
    CRISPR
    Tags
    FLAG and NLS
    Expression
    Mammalian
    Mutation
    Contains Brex27 sequence range is 4958-5065
    Promoter
    CMV
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    162758-plasmid-map-sequence-id-329432

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  13. dSpCas9-VPR

    Plasmid
    #205247
    Purpose
    In vitro transcription plasmid for production of dSpCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #63798.
    Depositor
    Rasmus Bak
    Publication
    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d
    Insert
    dSpCas9-VPR
    Use
    CRISPR
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    205247-plasmid-map-sequence-id-408440

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  14. pZ8-P_dCas9

    Plasmid
    #74063
    Purpose
    pZ8-1 plasmid carrying dcas9 driven by the propionate-inducible prpD2 promoter (PprpD2), KanR
    Depositor
    Timothy Lu
    Publication
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    dcas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Propionate inducible promoter (prp)
    Available since
    April 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    74063-plasmid-map-sequence-id-141970

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  16. pCC_11 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9-NLS-2A-Puro-WPRE

    Plasmid
    #139096
    Purpose
    Expresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dxCas9 3.7
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T, R324L,S409I, E480K, E543D, M6…
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139096-plasmid-map-sequence-id-273894

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  17. p300 core-dCas9-CBP core

    Plasmid
    #179559
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of human p300 core (aa 1048-1664) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Publication
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of human p300 core (aa 1048-1664) & c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179559-plasmid-map-sequence-id-354771

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Showing: 7401 - 7420 of 26412 results