We narrowed to 7,771 results for: Pol
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Plasmid#228763PurposePlasmid expressing Cas9 and gRNAs for mouse Ift88 and Pkd2. Use for disruption of mouse Ift88 and Pkd2 in cultured cells.DepositorUseCRISPRTags3XFLAG and GFPExpressionMammalianPromoterhuman U6Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only
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pFB-Dual-MBP-NavAb (KAV/G94C/Q150C)
Plasmid#226884PurposeExpresses a mutant of the Arcobacter butzleri voltage-gated sodium channel fused to an MBP tag in insect cellsDepositorInsertNaVAb
TagsMBPExpressionInsectMutationR4A, N49K, L109A, M116VPromoterPolyhedrinAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
8V04
Plasmid#220879PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertTMPRSS2 (TMPRSS2 Human)
UseBaculovirus expresssionTagsEFVEHHHHHHHH and Honeybee melittin signalMutationS251D/R252D/Q253D/S254D/R255KPromoterPolyhedrinAvailable sinceSept. 30, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MERS Mpro - His6-SUMO with autoprocessing - MSAVMQ/SGLVKM---MGVVMQ/SGVRKVTYGTAHWL
Plasmid#204790PurposeBacterial expression of MERS MproDepositorInsertMERS Mpro
ExpressionBacterialMutationMSAVMQ/SGLVKMAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-MHL_TLE4:474-773
Plasmid#210929PurposeInsect expression of human TLE4 fragment I474 to Y773. N-terminal 6X His tag with TEV cleavage.DepositorInsertTLE4:I474-Y773 (TLE4 Human)
TagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-MHL_LRRK2:2141-2527
Plasmid#210899PurposeInsect expression of human LRRK2 fragment T2141 to E2527. N-terminal 6X His tag with TEV cleavage.DepositorInsertLRRK2:T2141-E2527 (LRRK2 Human)
TagsMHHHHHHSSGRENLYFQGExpressionInsectMutationM2397T natural variant ( VAR_024965)PromoterPolyhedrinAvailable sinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pFBD-BirA_ATG16L1:303-607
Plasmid#210942PurposeInsect expression of human ATG16L1 fragment G303 to Y607. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertATG16L1:G303-Y607 (ATG16L1 Human)
TagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 4, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBD-BirA_CDC40:236-578
Plasmid#210885PurposeInsect expression of human CDC40 fragment E236 to W578. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertCDC40:E236-W578 (CDC40 Human)
TagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLX304-EZH1-Y727F
Plasmid#203582PurposeExpresses V5-tagged mutant version of EZH1 partially resistant to JQ-EZ-05 in mammalian cells.DepositorInsertEZH1 (EZH1 Human)
UseLentiviralTagsV5-taggedExpressionMammalianMutationchanged Tyrosine 727 to Phenylalanine for partialā¦PromoterCMVAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX304-EZH1-dPSET
Plasmid#203586PurposeExpresses V5-tagged mutant version of EZH1 with deletion of aa 744-747 and partially resistant to JQ-EZ-05 in mammalian cells.DepositorInsertEZH1 (EZH1 Human)
UseLentiviralTagsV5-taggedExpressionMammalianMutationdeletion of aa 744-747 and partial resistance to ā¦PromoterCMVAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFR*-CUbo(K63R)-His6
Plasmid#212820PurposeConstitutive or doxycycline-inducible expression of EGFR*-CUbo(K63R)-His6 in mammalian cellsDepositorAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFR*-CUbo(K48R)-GFP
Plasmid#212816PurposeConstitutive or doxycycline-inducible expression of EGFR*-CUbo(K48R)-GFP in mammalian cellsDepositorAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFR*-CUbo(K63R)-GFP
Plasmid#212817PurposeConstitutive or doxycycline-inducible expression of EGFR*-CUbo(K63R)-GFP in mammalian cellsDepositorAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
rUBR5_2383-2442_pGEX6P1
Plasmid#209791PurposeBacterial expression of UBR5 Mlle domainDepositorInsertMlle domain of UBR5 (Ubr5 Rat)
TagsGSTExpressionBacterialMutationaa 2383-2442 onlyPromotertacAvailable sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-HA_aa 321-843 REV1
Plasmid#201587PurposeExpresses the catalytic domain (aa 321-843) of human DNA repair protein REV1 with a HA tag in mammalian cellsDepositorInsertDNA repair protein REV1 (REV1 Human)
TagsHAExpressionMammalianMutationExpresses amino acids 321-843 of DNA repair proteā¦Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-rox-inv[Talpha1-iCre-pA]-rox-mNeonGreen-hCentrin1
Plasmid#196892PurposeNeuron-specific expression of human (h)Centrin fused to mNeonGreen. Used in combination with Talpha1-Dre-pA plasmidsDepositorAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-inv[Talpha1-iCre-pA]-lox-2xHA-hCentrin1
Plasmid#196891PurposeNeuron-specific expression of human (h)Centrin fused to 2xHA-tagsDepositorAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-inv[Talpha1-Dre-pA]-lox-mOrange2-Cdk5rap2-CTD
Plasmid#196881PurposeNeuron-specific expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.DepositorInsertmOrange2-Cdk5rap2-CTD
UseCre/Lox; Dre/roxExpressionMammalianPromoterQuimeric CAGAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-DIO-inv[roxSTOProx-Lyn-GFP-bGH]
Plasmid#196884PurposeDual Cre/Dre-dependent expression of LynGFP. Used as Cre-Dre cotransfection reporterDepositorInsertroxSTOProx-Lyn-GFP-bGH
UseCre/Lox; Dre/roxExpressionMammalianPromoterQuimeric CAGAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBactin-AcGFP-Cdk5rap2-CTDdeltaCBD
Plasmid#196856PurposeExpression of the centrosome targeting carboxy-terminal domain (CTD) laking the centrosome binding domain (deltaCBD) of Cdk5rap2 fused to AcGFP. It cannot displace Cdk5rap2 from centrosomesDepositorInsertAcGFP-Cdk5rap2-CTDdeltaCBD (Cdk5rap2 Rat)
ExpressionMammalianMutationLacking the centrosome-targeting domain (CTD)PromoterChicken bactin (plus Chicken bactin intron)Available sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only