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Anti-Pannexin-2 [N121A/31R]

Plasmid

#140073

Purpose

Mammalian Expression Plasmid of anti-Pannexin-2 (Rat). Derived from hybridoma N121A/31.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pannexin-2 (Rat) recombinant mouse monoclonal antibody (Panx2 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-HsNot9_19-285_AF

Plasmid

#148733

Purpose

Bacterial Expression of HsNot9_19-285

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

HsNot9_19-285 (CNOT9 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pC1-IMS-oROS-HT

Plasmid

#216419

Purpose

Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the inter-membrane-space of mitochondria.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

oROS-HT

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pF710-pETDuet-1-Hs-delta19-ETFalpha-noHis wt

Plasmid

#85111

Purpose

expression of recombinant human mature (delta19) ETFalpha wt without tag

Depositor

Pal Falnes

Article

Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

Insert

human mature (delta19) ETFalpha (ETFA Human)

Expression

Bacterial

Promoter

T7

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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VN-Tau (wt)

Plasmid

#87368

Purpose

expresses hTau fused to the N-terminal part of Venus

Depositor

Tiago Outeiro

Article

Blum et al Hum Mol Genet. 2015 Jan 1;24(1):76-85. d

Insert

MAPT (MAPT Human)

Tags

N-terminal part of Venus

Expression

Mammalian

Promoter

CMV

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-CD86

Plasmid

#223604

Purpose

Lentiviral expression of human CD86 in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD86 (CD86 Human)

Use

Lentiviral; mammalian expression

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-BirAopt-GSQ-RNF4wt-P2A-BLAST

Plasmid

#208046

Purpose

Enables constitutive expression of N-terminal BirAopt-fused RNF4wt; to perform bioE3; selection with blasticidin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

Insert

RNF4 (RNF4 Human)

Use

Lentiviral

Tags

BirAopt

Promoter

EFS

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Fon TVA-mCherry

Plasmid

#131779

Purpose

INTRSECT 2.0

Depositor

Karl Deisseroth

Article

Hafner et al Cell Rep. 2019 Sep 24;28(13):3450-3461.e

Insert

TVA-mCherry

Use

AAV; Intrsect

Tags

mCherry

Expression

Mammalian

Promoter

nEF

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST RBM14-V5

Plasmid

#69823

Purpose

Expresses RBM14-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

RNA-binding protein 14 (RBM14 Human)

Use

Retroviral

Tags

v5

Expression

Mammalian

Promoter

cmv

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST MTFR1L-V5

Plasmid

#69822

Purpose

Expresses MTFR1L-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Mitochondrial fission regulator 1-like (MTFR1L Human)

Use

Retroviral

Tags

v5

Expression

Mammalian

Promoter

cmv

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-SNAP-PDL1

Plasmid

#223602

Purpose

Lentiviral expression of human PD-L1 with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

PD-L1 (CD274 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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MAC_N_LMNA

Plasmid

#111688

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

LMNA (LMNA Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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Orf3a-mCherry

Plasmid

#165138

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Orf3a (ORF3a codon optimized SARS-COV-2)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pWPI-LUC7L2-3xFLAG

Plasmid

#201641

Purpose

Over-expression of 3xFLAG-tagged human LUC7L2

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

LUC7L2 (LUC7L2 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pBa-flag-BicD2 594-FKBP

Plasmid

#64206

Purpose

Bicaudal D protein aa1-594 (excluding start Met) with N-terminal flag tag and C-terminal FKBP

Depositor

Gary Banker, Marvin Bentley

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

Bicaudal D protein (Bicd2 Mouse)

Tags

FKBP and flag

Expression

Mammalian

Mutation

no start methionine, aa1-594

Promoter

chicken Beta Actin

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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pHR-CD86-SmBiT

Plasmid

#223618

Purpose

Lentiviral expression of human CD86 with SmBiT tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD86 (CD86 Human)

Use

Lentiviral; mammalian expression

Tags

SmBiT

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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LV-hHOXA13

Plasmid

#135561

Purpose

Express hHOXA13 and EGFP in mammalian cells

Depositor

Jose Teixeira

Article

George et al Cell Rep. 2019 Dec 17;29(12):4069-4085.e

Insert

Homeobox A13 (HOXA13 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CBH

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3-PAX3-FOXO1/FKHR

Plasmid

#115526

Purpose

Mammalian expression construct

Depositor

Martine Roussel

Article

Lam et al Mol Cell Biol. 1999 Jan;19(1):594-601.

Insert

Pax3-FOXO1/FKHR (FOXO1, Pax3 Mouse, Human)

Expression

Mammalian

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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His-Precission-NEDD4L_C942S_pCDNA3.1(+)

Plasmid

#187729

Purpose

Express the catalytically inactive NEDD4L FL in mammalian cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L FL with C874 mutated to a serine (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Mammalian

Mutation

Cys874 mutated to a serine

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-SmBiT-CD80

Plasmid

#223614

Purpose

Lentiviral expression of human CD80 with SmBiT tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD80 (CD80 Human)

Use

Lentiviral; mammalian expression

Tags

SmBiT

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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