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Bo_NPTII:pCYP86A2:Gus_introns

Plasmid

#215179

Purpose

Evaluating guard cell specific promotors in Brassica

Depositor

Penny Hundleby

Article

Nguyen et al Plant Direct. 2025 Feb 12;9(2):e70045. d

Insert

Cytochrome p450 86a2 monooxygenase

Use

Synthetic Biology

Promoter

pCYP86A2

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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Bo_NPTII:pGC1:Gus_introns

Plasmid

#215181

Purpose

Evaluating guard cell specific promotors in Brassica

Depositor

Penny Hundleby

Article

Nguyen et al Plant Direct. 2025 Feb 12;9(2):e70045. d

Insert

GA-stimulated regulatory protein

Use

Synthetic Biology

Promoter

AtGC1

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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Bo_NPTII:pCER6:Gus_introns

Plasmid

#215182

Purpose

Evaluating guard cell specific promotors in Brassica

Depositor

Penny Hundleby

Article

Nguyen et al Plant Direct. 2025 Feb 12;9(2):e70045. d

Insert

3-ketoacyl-coa synthase6

Use

Synthetic Biology

Promoter

AtCER6

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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Bo_NPTII:pGstA1+WIRa:Gus_introns

Plasmid

#215176

Purpose

Evaluating guard cell specific promotors in Brassica

Depositor

Penny Hundleby

Article

Nguyen et al Plant Direct. 2025 Feb 12;9(2):e70045. d

Insert

Glutathione-S-transferase1 Wheat‐induced resistance1

Use

Synthetic Biology

Promoter

TaGstA1 fused with TaWIR1 (chimeric)

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-mNeonGreen-G418

Plasmid

#236484

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on G418.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mNeonGreen

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHA-MOV10

Plasmid

#177921

Purpose

Expresses MOV10 with N-terminal HA tag

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

MOV10 (MOV10 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-mStayGold-G418

Plasmid

#236487

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mStayGold and selection on G418.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mStayGold

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-mCherry-G418

Plasmid

#236490

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mCherry and selection on G418.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mCherry

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-Dendra2-G418

Plasmid

#236496

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with Dendra2 and selection on G418.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

Dendra2

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-mScarlet-G418

Plasmid

#236493

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on G418.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mScarlet

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-GFP-G418

Plasmid

#236478

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on G418.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

GFP

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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FUGW-RUSH-Strep-KDEL

Plasmid

#228527

Purpose

Lentiviral vector, cloning vector for Strep-KDEL-IRES in mammalian cells.

Depositor

Ginny Farias

Article

Li et al Nat Commun. 2024 Dec 30;15(1):10829. doi

Insert

Strep-KDEL

Use

Lentiviral

Tags

Streptavidin-KDEL

Expression

Mammalian

Promoter

Ubc

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pGL 4.79-mCherry

Plasmid

#220137

Purpose

promoterless vector

Depositor

Monika Gulia-Nuss

Article

Pham et al iScience. 2024 Jul 8;27(8):110468. doi:

Type

Empty backbone

Use

Ixodes scapularis

Promoter

No promoter

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-CLIPf

Plasmid

#200787

Purpose

For the mammalian expression of CLIPf-HisTag-FLAG fusion protein.

Depositor

Alison Twelvetrees

Article

Smith et al iScience. 2026 Feb 2;29(3):114875. doi:

Insert

CLIPf-HisTag-FLAG

Tags

CLIPf

Expression

Mammalian

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-SNAPf

Plasmid

#200788

Purpose

For the mammalian expression of SNAPf-HisTag-FLAG fusion protein.

Depositor

Alison Twelvetrees

Article

Smith et al iScience. 2026 Feb 2;29(3):114875. doi:

Insert

SNAPf-HisTag-FLAG

Tags

SNAPf

Expression

Mammalian

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pIRES2_hMela(CT+IL3 mGluR6)

Plasmid

#191343

Purpose

Optogenetic tool

Depositor

Sonja Kleinlogel

Article

Kralik et al Commun Biol. 2022 Oct 20;5(1):1116. doi:

Insert

hMela(CT+IL3 mGluR6)

Tags

IRES_Turbo

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-EGFP-nonstop

Plasmid

#226406

Purpose

Expresses EGFP without a stop codon that produces a GFP-tagged readthrough RQC substrate.

Depositor

Nico Dantuma

Article

Xu et al Commun Biol. 2024 Oct 8;7(1):1282. doi:

Insert

EGFP without stop codon

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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PGK EGFP-RAB3A-T86E

Plasmid

#206148

Purpose

PGK EGFP-RAB3A-T86E

Depositor

Erika Holzbaur

Article

Dou et al J Cell Biol. 2024 Jun 3;223(6):e20230709

Insert

RAB3A (RAB3A Human)

Tags

EGFP

Expression

Mammalian

Mutation

T86E

Promoter

PGK

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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PGK EGFP-RAB3A-T86A

Plasmid

#206149

Purpose

PGK EGFP-RAB3A-T86A

Depositor

Erika Holzbaur

Article

Dou et al J Cell Biol. 2024 Jun 3;223(6):e20230709

Insert

RAB3A (RAB3A Human)

Tags

EGFP

Expression

Mammalian

Mutation

T86A

Promoter

PGK

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-39S Light chain

Plasmid

#216297

Purpose

Expresses anti-HER2 39S Light chain in mammalian cells. To be paired with 39S heavy chain to form the anti-HER2 39S Fab

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

39S Light chain

Use

Affinity Reagent/ Antibody

Tags

Signal peptide

Expression

Mammalian

Promoter

T7 promoter

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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