We narrowed to 7,673 results for: ski

1. pV1386

   Plasmid

   #111445

   Purpose

   Solo vector pV1382 + sgScADE2

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgScADE2/stop-codon repair
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-shortSUMO1

   Plasmid

   #228951

   Purpose

   express a fragment of the human SUMO1 protein (residues 18-97 of UniProt P6316 with an N-terminal thrombin-cleavable tag, MGSSHHHHHHSSGLVPR|GSHM-, where | indicates the cleavage site). Codon-optimized

   Depositor

   Marcin Suskiewicz

   Insert

   SUMO1 (SUMO1 Human)
   

   Tags

   His tag and thrombine cleavage site : MGSSHHHHHHS…

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-17

   Promoter

   T7-lac

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-RANGAP1-CTD

   Plasmid

   #228947

   Purpose

   express the C-terminal domain of the human RANGAP1 protein (residues 418-587 of UniProt P46060 with the N-terminal thrombin-cleavable MGSSHHHHHHSSGLVPR|GSHM- tag). Codon-optimized

   Depositor

   Marcin Suskiewicz

   Insert

   RANGAP1-CTD (RANGAP1 Human)
   

   Tags

   His tag and thrombine cleavage site : MGSSHHHHHHS…

   Expression

   Bacterial

   Mutation

   contains aa 418-587 only

   Promoter

   T7-lac

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS316-SSD1-L1374

   Plasmid

   #226278

   Purpose

   Plasmid expressing the SSD1 allele from L-1374, under control of its native promoter

   Depositor

   Jolanda Van Leeuwen

   Article

   Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

   Insert

   SSD1 (SSD1 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRS315-SEC22-NCYC110

   Plasmid

   #226273

   Purpose

   Plasmid expressing the SEC22 allele from NCYC110, under control of its native promoter

   Depositor

   Jolanda Van Leeuwen

   Article

   Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

   Insert

   SEC22 (SEC22 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS315-SEC22-L1374

   Plasmid

   #226271

   Purpose

   Plasmid expressing the SEC22 allele from L-1374, under control of its native promoter

   Depositor

   Jolanda Van Leeuwen

   Article

   Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

   Insert

   SEC22 (SEC22 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS315-SEC22-UWOPS872421

   Plasmid

   #226272

   Purpose

   Plasmid expressing the SEC22 allele from UWOPS87-2421, under control of its native promoter

   Depositor

   Jolanda Van Leeuwen

   Article

   Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

   Insert

   SEC22 (SEC22 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS315-SEC22-S288C

   Plasmid

   #226270

   Purpose

   Plasmid expressing the SEC22 allele from S288C, under control of its native promoter

   Depositor

   Jolanda Van Leeuwen

   Article

   Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

   Insert

   SEC22 (SEC22 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS316-SSD1-NCYC110

   Plasmid

   #226279

   Purpose

   Plasmid expressing the SSD1 allele from NCYC110, under control of its native promoter

   Depositor

   Jolanda Van Leeuwen

   Article

   Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

   Insert

   SSD1 (SSD1 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. MARS-nGFP

    Plasmid

    #205234

    Purpose

    Expresses PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells

    Depositor

    Jeremy Baskin

    Insert

    PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody (PLEKHA5 Human)
    

    Tags

    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3-2xMARS-PLD

    Plasmid

    #205237

    Purpose

    Expresses two repeats of PLEKAH5 aa 143-271 (K163A and R164A) fused to phospholipase D from Streptomyces sp. PMF

    Depositor

    Jeremy Baskin

    Inserts

    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    phospholipase D (PLD)
    

    Tags

    Nuclear Export Sequence and mCherry

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET3a-FruK

    Plasmid

    #186256

    Purpose

    Overexpression of FruK for purification

    Depositor

    Liskin Swint-Kruse, Emile Van Schaftingen

    Article

    Veiga-da-Cunha et al Protein Expr Purif. 2000 Jun;19(1):48-52

    Insert

    1-phosphofructokinase (fruK E. coli)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. AraC-pHG165c WT

    Plasmid

    #170114

    Purpose

    Constitutively expressed, wild-type E coli AraC transcription activator; activated by L-Arabinose.

    Depositor

    Liskin Swint-Kruse

    Article

    Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

    Insert

    AraC
    

    Expression

    Bacterial

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMET17p-CCG 316

    Plasmid

    #131166

    Purpose

    For ScMET17 promoter regulated expression of mCherry-Cub-R-GFP (CCG) fused bait proteins, centromeric ARS plasmid, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutant

    Depositor

    Nils Johnsson

    Article

    Dunkler et al Methods Mol Biol. 2015;1346:151-68. doi:

    Type

    Empty backbone

    Tags

    CCG

    Expression

    Yeast

    Available Since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. LLhE_AW-pHG165 H118R

    Plasmid

    #90063

    Purpose

    Constitutive expression of a chimeric LacI:CelR transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Sousa et al J Mol Biol. 2016 Feb 22;428(4):671-8. do

    Insert

    LLhE_AW/H118R
    

    Expression

    Bacterial

    Mutation

    I48V/Q55A/Q60R/H118R

    Promoter

    Iq

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. LLhG-pHG165a

    Plasmid

    #90039

    Purpose

    Constitutive expression of a chimeric LacI:GalR transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Proteins. 2008 Dec;73(4):941-57. doi: 10

    Insert

    LLhG
    

    Expression

    Bacterial

    Promoter

    Iq

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. LGhG-pHG165a

    Plasmid

    #90048

    Purpose

    Constitutive expression of a chimeric LacI:GalR transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Tungtur et al J Mol Biol. 2010 Jan 29;395(4):785-802.

    Insert

    LGhG
    

    Expression

    Bacterial

    Promoter

    Iq

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. SMN2E7-Fluc-Nluc-reporter

    Plasmid

    #221005

    Purpose

    Transiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.

    Depositor

    Benjamin Blencowe, Mikko Taipale

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Insert

    Fluc-SMN2E7-STOP-Nluc
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. shh10

    Plasmid

    #64867

    Purpose

    This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.

    Depositor

    John Flannery, David Schaffer

    Article

    Klimczak et al PLoS One. 2009 Oct 14;4(10):e7467. doi:

    Insert

    shh10 cap
    

    Use

    AAV

    Mutation

    I319V, N451D, D532N

    Available Since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLJM1 EGFP-Tau

    Plasmid

    #108868

    Purpose

    Expresses EGFP linked to human Tau

    Depositor

    Gabriele Kaminski Schierle

    Insert

    Tau (MAPT Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    human four-repeat tau lacking the N-terminal sequ…

    Promoter

    CMV

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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