We narrowed to 168,552 results for: addgene

Showing: 7441 - 7460 of 168552 results

Results per page:

Sort results by:

pEIF3D-CtermDel-sgEIF3D

Plasmid

#222125

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

F527 truncation

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEIF3D-a5helixDel-sgEIF3D

Plasmid

#222133

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

D249Q, V262I, Y263A

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEIF3D-S528N-S529N-sgEIF3D

Plasmid

#222129

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

S528N, S529N

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
FgH1tUTG-sgRNA.FOXA-ZEB2_CRISPRd

Plasmid

#216170

Purpose

Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Insert

sgRNA- FOXA.bs- ZEB2.locus

Use

Lentiviral

Tags

EGFP

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
FgH1tUTG-sgRNA.Control-ZEB2_CRISPRd

Plasmid

#216172

Purpose

Express the gRNA targeting the control site in the human ZEB2 locus for the CRISPRd experiment

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Insert

sgRNA- Control- ZEB2.locus

Use

Lentiviral

Tags

EGFP

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LRG-sgRNA.FOXA-ZEB2_CRISPRd_v2

Plasmid

#216173

Purpose

Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Insert

sgRNA- FOXA.bs- ZEB2.locus

Use

Lentiviral

Tags

EGFP

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SCML2_3

Plasmid

#220878

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Article

Nady et al J Mol Biol. 2012 Nov 9;423(5):702-18. do

Insert

SCML2 (SCML2 Human)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

June 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pSCL475

Plasmid

#219503

Purpose

Express Ade2, Can1 and Faa1 retron msd donor-gRNAs from a single Gal7 promoter, separated by a Csy4 recognition sequence, with an msr expressed in trans

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

Insert

Ade2, Can1 and Faa1 retron msd donor-gRNAs separated by a Csy4 recognition sequence, with an msr expressed in trans

Expression

Yeast

Promoter

GAL7

Available Since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW57.1 WT1 +378 ATG

Plasmid

#217644

Purpose

Dox inducible WT1 cDNA - as transcribed from +378ATG (short isoform)

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

WT1 short isoform (WT1 Human)

Expression

Mammalian

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW57.1 Ex8 mut WT1

Plasmid

#217645

Purpose

Dox inducible WT1 cDNA with exon 8 mutation

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

WT1 (WT1 Human)

Expression

Mammalian

Mutation

Frameshift exon 8

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW57.1 Ex9 mut WT1

Plasmid

#217646

Purpose

Dox inducible WT1 cDNA with exon 9 mutation

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

WT1 (WT1 Human)

Expression

Mammalian

Mutation

Frameshift exon 9

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBDP

Plasmid

#217827

Purpose

Stuffer plasmid with golden gate compatible bidirectional promoter

Depositor

Timothy Whitehead

Article

Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

Insert

Gal1/Gal10 Bidirectional Promoter

Use

Cloning vector

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNS49 (enAsCas12a-KRAB piggyBac)

Plasmid

#217335

Purpose

piggyBac expresion of enAsCas12a-KRAB

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

enAsCas12a

Use

CRISPR

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Expression

Mammalian

Mutation

E174R/S542R/K548R

Promoter

CAG

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-mKO2-Parkin

Plasmid

#213544

Purpose

Transient mammalian expresssion of Parkin, N-terminally tagged with mKO2, M1 of Parkin intact

Depositor

Steven Altschuler, Lani Wu

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Tags

mKO2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Parkin(E16A)

Plasmid

#213546

Purpose

Transient mammalian expresssion of E16A Parkin

Depositor

Steven Altschuler, Lani Wu

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Expression

Mammalian

Mutation

E16A mutation in Parkin

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP782_Traptavidin-KRAB-CO

Plasmid

#211775

Purpose

AviTag counterpart binding domain, Traptavidin, fused to transcriptional repressor KRAB, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

SpyCatcher-DNMT3A

Tags

3xFLAG

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect DNMT3A…

Promoter

pEF1a and pSV40

Available Since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP785_Traptavidin-DNMT3A-CO

Plasmid

#211778

Purpose

AviTag counterpart binding domain, Traptavidin, fused to DNA methyltransferase DNMT3A, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

Traptavidin-EZH2

Tags

3xV5

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect EZH2 e…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP781_SnoopCatcher-KRAB-CO

Plasmid

#211774

Purpose

SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional repressor KRAB, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

Traptavidin-KRAB

Tags

3xV5

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect KRAB e…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
dCas9-RPT6-FLAG

Plasmid

#205416

Purpose

Expresses dCas9-RPT6 with FLAG label

Depositor

Timothy Jarome

Article

Farrell et al J Neurosci. 2024 Jan 24;44(4):e145323202

Insert

dCas9-RPT6 (Psmc5 Rat)

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
phROSA26-Tet-HA-JLP_V55Q

Plasmid

#208771

Purpose

Tetracycline inducible expression vector for HA-JLP_V55Q mutant protein, used as a donor plasmid for HA-JLP_V55Q knock-in at the human ROSA26 locus

Depositor

Katsuji Yoshioka

Article

Suzuki et al Genes Cells. 2023 Nov 14. doi: 10.1111/g

Insert

JLP (SPAG9 Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge