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pHH0103_ASAP2-1/1

Plasmid

#91504

Purpose

Protein expression and purification of human SH3 domain construct ASAP2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ASAP2-1/1 (ASAP2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_MAP3K9-1/2

Plasmid

#91313

Purpose

Protein expression and purification of human SH3 domain construct MAP3K9-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MAP3K9-1/2 (MAP3K9 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGAP12-1/1

Plasmid

#91282

Purpose

Protein expression and purification of human SH3 domain construct ARHGAP12-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGAP12-1/1 (ARHGAP12 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_AHI1-1/1

Plasmid

#91269

Purpose

Protein expression and purification of human SH3 domain construct AHI1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

AHI1-1/1 (AHI1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pESC-LEU-SopB

Plasmid

#183670

Purpose

Inducible Salmonella Typhimurium SopB for expression in yeast

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Expression

Yeast

Promoter

GAL1

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pESC-Leu-SopB(C460S)

Plasmid

#183671

Purpose

Inducible Salmonella Typhimurium SopB (catalytically inactive) for expression in yeast

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Expression

Yeast

Mutation

C460S

Promoter

GAL1

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGEF9-1/1

Plasmid

#91417

Purpose

Protein expression and purification of human SH3 domain construct ARHGEF9-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGEF9-1/1 (ARHGEF9 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pPGK-T7/2-CD44v9 (-cyt)

Plasmid

#137819

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v9 (-cyt) (CD44 Human)

Tags

GFP

Expression

Mammalian

Mutation

without cytoplasmic region

Promoter

PGK

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pEMS2260

Plasmid

#158423

Purpose

AAV plasmid with Ple331 (PAX6 MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple331 (PAX6 Human)

Use

AAV

Promoter

Ple331

Available Since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pMS04 [myo-3p::bPGC::SL2::mCherry]

Plasmid

#168172

Purpose

Expression of bPGC in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

Insert

bPGC

Expression

Worm

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-TOX2

Plasmid

#192920

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of TOX2

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

TOX2 (TOX2 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGEF6-1/1

Plasmid

#91515

Purpose

Protein expression and purification of human SH3 domain construct ARHGEF6-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGEF6-1/1 (ARHGEF6 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-E2F2 3'UTR

Plasmid

#29469

Depositor

Judy Lieberman

Article

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

E2F2 3'UTR (E2F2 Human)

Expression

Mammalian

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
mTFP1-MAPTau-N-10

Plasmid

#55498

Purpose

Localization: Microtubules, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

MAPTau (MAPT Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
Cas9_YTHDF2_sgRNA

Plasmid

#186673

Purpose

YTHDF2 sgRNA plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

YTHDF2 KO sgRNA Plasmid (YTHDF2 Human)

Expression

Mammalian

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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human TLNRD1-F250D pET151

Plasmid

#159385

Purpose

Expresses human TLNRD1 F250D mutant in bacterial cells

Depositor

Ben Goult

Article

Cowell et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

Talin Rod Domain Containing Protein 1 F250D mutant (TLNRD1 Human)

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Mutation

Changed Phenylalanine 250 to Aspartic Acid (F250D)

Promoter

T7

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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lenti-Cas9-VQR-GFP_activity_reporter

Plasmid

#87156

Purpose

This lentiviral vector can be used to assay activity of SpCas9-VQR (NGA PAM restricted).

Depositor

Daniel Bauer

Article

Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

Insert

GFP and sgRNA targeting GFP (NGA restricted)

Use

Lentiviral

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CARMA2cl-FLAG

Plasmid

#97077

Purpose

Expresses CARMA2cl/CARD14 isoform 2 in mammalian cells

Depositor

Pasquale Vito

Article

Scudiero et al J Cell Physiol. 2011 Dec;226(12):3121-31

Insert

CARD14 isoform 2 (CARD14 Human)

Tags

FLAG

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pS6-C-hα2M-H6

Plasmid

#128497

Purpose

Expresses human C-α2M (from F789 to S1468) in mammalian cells. With C-t H6x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

A2M (F789-S1468) (A2M Human)

Tags

His tag (6x)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pαH-S-HRV3C/D614G

Plasmid

#164568

Purpose

SARS-CoV-2 Spike protein with furin site mutated to HRV3C site and D614G mutation (S-HRV3C-D614G variant)

Depositor

Priyamvada Acharya

Article

Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

Insert

Spike (S-HRV3C-D614G variant)

Tags

2X Strep-Tag II and 8X His tag

Expression

Mammalian

Mutation

D614G

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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