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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,499 results for: addgene/1000

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Showing: 7461 - 7480 of 7499 results

  1. MPC1-QQ-FLAG

    Plasmid
    #228905
    Purpose
    Expression of mouse MPC1 in which K45 and K46 motif sites were replaced with glutamine to mimic acetylated lysines with a C terminal FLAG tag
    Depositor
    Ghazaleh Ashrafi
    Article
    Tiwari et al Sci Adv. 2024 Nov 15;10(46):eadp7423. do
    Insert
    MPC1 (Mpc1 Mouse)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    K45 and K46 motif sites were both replaced with g…
    Promoter
    CMV
    Available Since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228905-plasmid-map-sequence-id-460266

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  3. TFORF3066

    Plasmid
    #144542
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CXXC1 (CXXC1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144542-plasmid-map-sequence-id-353564

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  11. pINDUCER20_mStrawberry_Atg4BC74A

    Plasmid
    #161735
    Purpose
    Doxycycline-inducible expression of Atg4B(C74A) fused with mStrawberry to inhibit autophagy
    Depositor
    Alec Kimmelman
    Article
    Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:
    Insert
    Atg4B(C74A) (Atg4b Mouse) (Atg4b Mouse)
    Use
    Lentiviral; Destinatioin vector for gateway cloni…
    Tags
    mStrawberry
    Expression
    Mammalian
    Mutation
    220-222 TGC (Cys) is changed to GCA (Ala)
    Available Since
    Dec. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    161735-plasmid-map-sequence-id-320115

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  12. pSLIK_Hyg_mTurquoise2_Atg4BC74A

    Plasmid
    #161733
    Purpose
    Doxycycline-inducible expression of Atg4B(C74A) fused with mTurquoise2 to inhibit autophagy
    Depositor
    Alec Kimmelman
    Article
    Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:
    Insert
    Atg4B(C74A) (Atg4b Mouse) (Atg4b Mouse)
    Use
    Lentiviral; Destinatioin vector for gateway cloni…
    Tags
    mTurquoise2
    Expression
    Mammalian
    Mutation
    220-222 TGC (Cys) is changed to GCA (Ala)
    Available Since
    Nov. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    161733-plasmid-map-sequence-id-318425

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  13. pOTTC1845 - pAAV CaMKII hChR2(H134R)-V5-ER-LBD (ChRERa)

    Plasmid
    #201822
    Purpose
    An adeno-associated viral vector expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a CaMKii promoter, for use in PET imaging
    Depositor
    Christopher Richie
    Article
    Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd
    Insert
    hChR2(H134R)-V5-ER-LBD (ChRERa)
    Use
    AAV
    Promoter
    CaMKii
    Available Since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    201822-plasmid-map-sequence-id-399617

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  16. G protein alpha-q-GFP

    Plasmid
    #66080
    Purpose
    This G protein alpha-q construct contains internal insertions of GFP and the EE epitope
    Depositor
    Catherine Berlot
    Article
    Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.
    Insert
    G protein alpha-q-EE-YFP (Gnaq Mouse)
    Tags
    GFP was inserted internally into the alpha-q sequ…
    Expression
    Mammalian
    Mutation
    BamHI and SacI sites in alpha-q were removed with…
    Promoter
    CMV
    Available Since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    66080-plasmid-map-sequence-id-436984

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  18. lucMAPT-30D

    Plasmid
    #214672
    Purpose
    MAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs downstream of the 5′ splice site
    Depositor
    Eugene Yeo
    Article
    Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Insert
    MAPT (MAPT Human)
    Tags
    Firefly Luciferase, Renilla Luciferase, and V5
    Expression
    Mammalian
    Mutation
    Contains Exon 9, Exon 10 with a mutation to intro…
    Promoter
    CMV
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214672-plasmid-map-sequence-id-424908

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  19. lucMAPT-30U

    Plasmid
    #214673
    Purpose
    MAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs upstream of the 3′ splice site
    Depositor
    Eugene Yeo
    Article
    Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Insert
    MAPT (MAPT Human)
    Tags
    Firefly Luciferase, Renilla Luciferase, and V5
    Expression
    Mammalian
    Mutation
    Contains Exon 9, Exon 10 with a mutation to intro…
    Promoter
    CMV
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214673-plasmid-map-sequence-id-424933

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  20. G protein alpha-q-CFP

    Plasmid
    #66081
    Purpose
    This G protein alpha-q construct contains internal insertions of CFP and the EE epitope
    Depositor
    Catherine Berlot
    Article
    Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.
    Insert
    G protein alpha-q-EE-CFP (Gnaq Mouse)
    Tags
    CFP was inserted internally into the alpha-q sequ…
    Expression
    Mammalian
    Mutation
    BamHI and SacI sites in alpha-q were removed with…
    Promoter
    CMV
    Available Since
    July 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    66081-plasmid-map-sequence-id-118921

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Showing: 7461 - 7480 of 7499 results