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PINK1-SPARK

Plasmid

#248086

Purpose

PINK1 phase separation-based biosensor

Depositor

Danielle Schmitt

Article

Vineall et al bioRxiv [Preprint]. 2025 Oct 16:2025.10.

Insert

PINK1-SPARK

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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SM425_pHAGE-CAG-mcherry-P2A-puro-DR-Lb_crRNA-KAN-DR

Plasmid

#244225

Purpose

Vector for golden gate cloning LbCas12a crRNA arrays. Contains mCherry-P2A-puroR selectable marker.

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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SM426_pHAGE-CAG-mCherry-T2A-puro-DR-As_crRNA-KAN-DR

Plasmid

#244226

Purpose

Vector for golden gate cloning AsCas12a crRNA arrays. Contains mCherry-P2A-puroR selectable marker.

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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SM271_EF1A-dHyperLbCas12a-4xSID-T2A-BlastR

Plasmid

#244209

Purpose

Mammalian expression of dHyperLbCas12a-4xSID with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dHyperLbCas12a-4xSID

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDL1-Spol-foxA-C-term

Plasmid

#242944

Purpose

Encodes a C-terminal fragment of Spol-foxA to generate dsRNA for RNAi experiments in the planarian S. polychroa

Depositor

Erin Davies

Article

Deihl et al bioRxiv [Preprint]. 2025 Jul 22:2025.07.

Insert

Spol-foxA

Use

RNAi

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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SM272_EF1A-dHyperLbCas12a-VPR-T2A-EGFP

Plasmid

#244210

Purpose

Mammalian expression of dHyperLbCas12a-VPR with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dHyperLbCas12a-VPR

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM274_EF1A-dEnAsCas12a-KRAB-T2A-EGFP

Plasmid

#244212

Purpose

Mammalian expression of dEnAsCas12a-KRAB with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dEnAsCas12a-KRAB

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM270_EF1A-dHyperLbCas12a-4xSID-T2A-EGFP

Plasmid

#244208

Purpose

Mammalian expression of dHyperLbCas12a-4xSID with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dHyperLbCas12a-4xSID

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM266_EF1A-dHyperLbCas12a-KRAB-T2A-BlastR

Plasmid

#244205

Purpose

Mammalian expression of dHyperLbCas12a-KRAB with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dHyperLbCas12a-KRAB

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM275_EF1A-dEnAsCas12a-KRAB-T2A-BlastR

Plasmid

#244213

Purpose

Mammalian expression of dEnAsCas12a-KRAB with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dEnAsCas12a-KRAB

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM273_EF1A-dHyperLbCas12a-VPR-T2A-BlastR

Plasmid

#244211

Purpose

Mammalian expression of dHyperLbCas12a-VPR with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dHyperLbCas12a-VPR

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM280_EF1A-dEnAsCas12a-4xSID-T2A-BlastR

Plasmid

#244215

Purpose

Mammalian expression of dEnAsCas12a-4xSID with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dEnAsCas12a-4xSID

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM279_EF1A-dEnAsCas12a-4xSID-T2A-EGFP

Plasmid

#244214

Purpose

Mammalian expression of dEnAsCas12a-4xSID with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dEnAsCas12a-4xSID

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDRF1 Cyterm-mGold

Plasmid

#224084

Purpose

Expresses Cyterm-mGold to check for monomericity.

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

Cyterm-mGold

Expression

Yeast

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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P-fusA2

Plasmid

#235656

Purpose

Reports Cur activity in Bacteroides thetaiotaomicron

Depositor

Guy Townsend

Article

Kabonick et al bioRxiv [Preprint]. 2024 Nov 13:2024.11.

Insert

Bacteroides thetaiotaomicron fusA2 promoter

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-T4gp46

Plasmid

#228211

Purpose

Expression in BL21(DE3) of T4 SbcC-like subunit of palindrome specific endonuclease

Depositor

Stephen Benkovic

Article

Herdendorf et al J Biol Chem. 2011 Jan 28;286(4):2382-92.

Insert

46

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pZZMP132

Plasmid

#231757

Purpose

Mammalian expression of SPO11-EA

Depositor

Scott Keeney

Article

Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

Insert

SPO11-EA (Spo11 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

E224A

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2837

Plasmid

#231676

Purpose

Expresses a rabbit IgH signal peptide fused to GFP in mammalian cells

Depositor

Damian Ekiert

Article

Jaroenlak et al bioRxiv [Preprint]. 2024 May 30:2024.05.

Insert

Rabbit IgH-sfGFP

Tags

sfGFP

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pJB75

Plasmid

#226320

Purpose

E. coli protein expression for protein purification

Depositor

David Savage

Article

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

6xHis-(Y to A MR1-6)-strep

Tags

6xHis and Strep

Expression

Bacterial

Mutation

N-terminal domain deleted, C-terminal domain dele…

Promoter

T7

Available Since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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EphA1-SAM-Cry2olig

Plasmid

#176600

Purpose

EphrinA1 RTK fusion to Cry2olig - includes SAM domain

Depositor

Robert Hughes

Article

Wurz et al bioRxiv [Preprint]. 2024 Feb 7:2024.02.0

Insert

EphrinA1-Cry2olig-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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