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pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q-P70C

Plasmid

#114629

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P70C cysteine mutations and a 49 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P70C; 49 polyQ repeat

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-23Q-A2C-P90C

Plasmid

#114615

Purpose

Bacterial expression of Htt fragment containing exon 1, with A2C & P90C cysteine mutations and a 23 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P90C; 23 polyQ repeat

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q-A60C

Plasmid

#114628

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 49 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, A60C; 49 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90-37Q-P90C

Plasmid

#114621

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 37 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

P90C; 37 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-23Q-P80C

Plasmid

#114614

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 23 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P80C; 23 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-43Q-P70C

Plasmid

#114624

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P70C cysteine mutations and a 43 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P70C; 43 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-43Q-A2C-P90C

Plasmid

#114625

Purpose

Bacterial expression of Htt fragment containing exon 1, with A2C & P90C cysteine mutations and a 43 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P90C; 43 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q-P90C

Plasmid

#114630

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 49 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P90C; 49 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-P90C

Plasmid

#114610

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 15 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P90C; 15 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-P70C

Plasmid

#114609

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P70C cysteine mutations and a 15 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P70C; 15 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-P90C

Plasmid

#114620

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 37 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P90C; 37 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-P80C

Plasmid

#114619

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P80C cysteine mutations and a 37 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P80C; 37 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-A60C

Plasmid

#114618

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 37 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, A60C; 37 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-A60C

Plasmid

#114608

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 15 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, A60C; 15 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Htt2-90(A2C)-23Q

Plasmid

#114612

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C cysteine mutation and a 23 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C; 23 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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GoldenBraid 2.0 Kit

Plasmid Kit

#1000000076

Purpose

Modular cloning of multigenic DNA constructs in Plant Synthetic Biology applications. 95 GoldenBraid cloning compatible plasmids, containing standard basic parts and transcriptional units

Depositor

Diego Orzaez

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only
pLV x EF1a_EKAREN5-ires-puro

Plasmid

#167821

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLV x EF1a_EKAREN4-ires-puro

Plasmid

#167828

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-dCas9-p300-P2A-PuroR

Plasmid

#83889

Purpose

Lentiviral Sp dCas9-p300-P2A-PuroR

Depositor

Charles Gersbach

Article

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
pac from Streptomyces alboniger

Use

CRISPR and Lentiviral

Tags

Flag and nucleoplasmin NLS

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

EFS

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-α-cateninWT

Plasmid

#229709

Purpose

Transient expression of GFP-alpha-cateninWT in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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