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pUCSh3-2300

Plasmid

#170985

Purpose

Encodes sgRNA target 2300 and Tetracycline resistance transposon.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

Sh-CAST sgRNA 2300

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pUCSh8-2300

Plasmid

#170986

Purpose

Encodes sgRNA target 2300 and Hygromycin resistance transposon.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

Sh-CAST sgRNA 2300

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6Biso1-delMI-MII_F

Plasmid

#146304

Purpose

Mammalian Expression of HsTNRC6Biso1-delMI-MII

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6Biso1-delMI-MII (TNRC6B Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6C-delMI-AGOhook_F

Plasmid

#146309

Purpose

Mammalian Expression of HsTNRC6C-delMI-AGOhook

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6C-delMI-AGOhook (TNRC6C Human)

Expression

Mammalian

Mutation

1 silent mutations compared to the sequence of Hs…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6C-delMI-MII_F

Plasmid

#146311

Purpose

Mammalian Expression of HsTNRC6C-delMI-MII

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6C-delMI-MII (TNRC6C Human)

Expression

Mammalian

Mutation

2 silent mutations compared to the sequence of Hs…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6C-delMI-MII-AGOhook_F

Plasmid

#146312

Purpose

Mammalian Expression of HsTNRC6C-delMI-MII-AGOhook

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6C-delMI-MII-AGOhook (TNRC6C Human)

Expression

Mammalian

Mutation

2 silent mutations compared to the sequence of Hs…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot6-159-557_AF

Plasmid

#148657

Purpose

Bacterial Expression of HsNot6_159-557

Depositor

Elisa Izaurralde

Article

Chen et al Nucleic Acids Res. 2021 Jun 21;49(11):64

Insert

HsNot6_159-557 (CNOT6 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot6-D491N_AF

Plasmid

#148660

Purpose

Bacterial Expression of HsNot6-D491N

Depositor

Elisa Izaurralde

Article

Chen et al Nucleic Acids Res. 2021 Jun 21;49(11):64

Insert

HsNot6-D491N (CNOT6 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot6-R65E_AF

Plasmid

#148663

Purpose

Bacterial Expression of HsNot6-R65E

Depositor

Elisa Izaurralde

Article

Chen et al Nucleic Acids Res. 2021 Jun 21;49(11):64

Insert

HsNot6-R65E (CNOT6 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot6-R87T_AF

Plasmid

#148665

Purpose

Bacterial Expression of HsNot6-R87T

Depositor

Elisa Izaurralde

Article

Chen et al Nucleic Acids Res. 2021 Jun 21;49(11):64

Insert

HsNot6-R87T (CNOT6 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-HsNot7-NpM-HsNot6_AF

Plasmid

#148645

Purpose

Bacterial Expression of HsNot7-HsNot6

Depositor

Elisa Izaurralde

Article

Chen et al Nucleic Acids Res. 2021 Jun 21;49(11):64

Insert

HsNot7-HsNot6 (CNOT7 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-delMI-MII_F

Plasmid

#146298

Purpose

Mammalian Expression of HsTNRC6A-delMI-MII

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6A-delMI-MII (TNRC6A Human)

Expression

Mammalian

Mutation

Five non silent mutations A339T, V417M, R934G, D1…

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-delMI-MII-AGOhook_F

Plasmid

#146299

Purpose

Mammalian Expression of HsTNRC6A-delMI-MII-AGOhook

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6A-delMI-MII-AGOhook (TNRC6A Human)

Expression

Mammalian

Mutation

Four non silent mutations A339T, R934G, D1289G, a…

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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Dcp2C Δ5H

Plasmid

#163581

Purpose

express Dcp2C Δ5H at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

Truncation of aa1-300; mutate aa440-450, aa489-50…

Promoter

DCP2 promoter

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pVG88

Plasmid

#164709

Purpose

yEVenus under the control of ARG3 promoter

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

Insert

ARG3pr - yEVenus - CLN2 PEST - ADH1t

Expression

Yeast

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pFTtG5

Plasmid

#149488

Purpose

Broad-Host range expression vector, RSF1010 origin, TtgR(AM) repressor, PTtg promoter, mRFP.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

TtgR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PTtg

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCL10

Plasmid

#164710

Purpose

yEVenus under the control of MET3 promoter

Depositor

Fred Cross

Article

Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

Insert

MET3pr - yEVenus - CLN2 PEST - ADH1t

Expression

Yeast

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2 300

Plasmid

#163577

Purpose

express Dcp2 300 at endogenous level in S.cerevisiae

Depositor

Roy Parker

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

Truncation of C-terminal domian (301-970)

Promoter

DCP2 promoter

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-K295TAG-W340A.pcDNA3-k

Plasmid

#126627

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site.

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid mutations: K295 to Amber codon and W34…

Promoter

CMV

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-E322TAG-E309C-W340A-CAAX.pcDNA3-k

Plasmid

#127409

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

CAAX and FLAG

Expression

Mammalian

Mutation

Amino acid mutations: E322 to Amber codon, E309C …

Promoter

CMV

Available Since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

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