We narrowed to 19,051 results for: Tec

1. pRSF-G1(5CNW)RS

   Plasmid

   #222872

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of 5-Cyano-L-Tryptophan (5-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

   Insert

   tRNA synthetase
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. RhoA gRNA #1

   Plasmid

   #198718

   Purpose

   Cas9-mediated knockout of RhoA in mammalian cells #1

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   RhoA (Rhoa Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. RhoA gRNA #3

   Plasmid

   #198720

   Purpose

   Cas9-mediated knockout of RhoA in mammalian cells #3

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   RhoA (Rhoa Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. RhoB gRNA #1

   Plasmid

   #198721

   Purpose

   Cas9-mediated knockout of RhoB in mammalian cells #1

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   RhoB (Rhob Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. RhoB gRNA #2

   Plasmid

   #198722

   Purpose

   Cas9-mediated knockout of RhoB in mammalian cells #2

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   RhoB (Rhob Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. RhoB gRNA #3

   Plasmid

   #198723

   Purpose

   Cas9-mediated knockout of RhoB in mammalian cells #3

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   RhoB (Rhob Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. RhoC gRNA #1

   Plasmid

   #198724

   Purpose

   Cas9-mediated knockout of RhoC in mammalian cells #1

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   RhoC (Rhoc Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. RhoC gRNA #2

   Plasmid

   #198725

   Purpose

   Cas9-mediated knockout of RhoC in mammalian cells #2

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   RhoC (Rhoc Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. RhoC gRNA #3

   Plasmid

   #198726

   Purpose

   Cas9-mediated knockout of RhoC in mammalian cells #3

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   RhoC (Rhoc Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR221-DXO

    Plasmid

    #177992

    Purpose

    For gateway cloning of DXO

    Depositor

    Karla Feijs

    Article

    Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

    Insert

    DXO (DXO Human)
    

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SpCas9-miniCGBE1

    Plasmid

    #198548

    Purpose

    Expresses human codon-optimized SpCas9-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-miniCGBE1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-miniCGBE1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. SpCas9-NG-miniCGBE1

    Plasmid

    #198538

    Purpose

    Expresses human codon-optimized SpCas9-NG-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-miniCGBE1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-miniCGBE1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. SpCas9-NRRH

    Plasmid

    #198540

    Purpose

    Expresses human codon-optimized SpCas9-NRRH and blasticidin resistance: EFS promoter-SpCas9-NRRH-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRRH
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SpCas9-NRCH

    Plasmid

    #198542

    Purpose

    Expresses human codon-optimized SpCas9-NRCH and blasticidin resistance: EFS promoter-SpCas9-NRCH-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR221-PARP11

    Plasmid

    #184469

    Purpose

    For gateway cloning of PARP11

    Depositor

    Karla Feijs

    Article

    Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

    Insert

    PARP11 (PARP11 Human)
    

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTRE3G-NS3/4A S139A

    Plasmid

    #186885

    Purpose

    Doxycycline-dependent expression of catalytically inactive NS3/4A protease gene (S140A) in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    HCV NS3/4A protease with S139A mutation
    

    Use

    Retroviral

    Mutation

    S139A

    Promoter

    TRE3G

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTRE3G-WHG cGAS-HA

    Plasmid

    #186867

    Purpose

    Doxycycline-dependent expression of white-handed gibbon cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    White-handed gibbon cGAS with C-terminal HA tag
    

    Use

    Retroviral

    Tags

    HA

    Promoter

    TRE3G

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYAMTr2GCsgSc-SeLEU2

    Plasmid

    #224871

    Purpose

    Disruption of LEU2 genes in the Saccharomyces sense strict group species

    Depositor

    Katsunori Suzuki

    Article

    Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

    Insert

    pYAMTr2GC having the guide sequence Sc-SeLEU2 (LEU2, DI49_0736 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDGB3_alpha1_CBS-FRT-OCS-BeYDVRepRepA-tNos (GB4646)

    Plasmid

    #225438

    Purpose

    Transcriptional unit for copper- and flipase-regulated BeYDV Rep/RepA. OCSt, flanked by FRT sites, is embedded in the 5'UTR of miniDFR.

    Depositor

    Diego Orzaez

    Article

    Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

    Insert

    CBS-FRT-OCS-BeYDVRepRepA-tNos
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDGB3_alpha2_BeYDV geminino 1.0 (GB4480)

    Plasmid

    #225436

    Purpose

    BeYDV LIR1:2nd intron+eGFP CDS+Ter35S+p35S+1st intron:LIR1 in alpha2. Deconstructed replicon that needs BeYDV Rep for circularization and, thus, replication and eGFP transcription.

    Depositor

    Diego Orzaez

    Article

    Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

    Insert

    BeYDV geminino 1.0
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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