We narrowed to 7,673 results for: ski

1. pBrain-GFP-TACC3KDP(L566,567A)-shTACC3

   Plasmid

   #69117

   Purpose

   Dual-promoter plasmid to express knockdown-proof human TACC3 (L566A and L567A substitutions) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.

   Depositor

   Stephen Royle

   Article

   Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

   Inserts

   Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
   shTACC3
   

   Use

   RNAi

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   LL (566-567aa) mutated to AA. Silent mutations i…

   Promoter

   CMV

   Available Since

   Feb. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEM BirA-2A-Citrine-SV40pA-FRT-Kan-FRT

   Plasmid

   #89890

   Purpose

   BAC donor construct containing 3XHA-tagged BirA, a ribosome skipping motif - 2A, Citrine reporter, polyadenyation signal, followed by FRT recombination sites flanking kanamycin selection cassette.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

   Insert

   HA-BirA-2A-citrine
   

   Use

   Unspecified

   Tags

   Citrine

   Available Since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBrain-GFP-TACC3KDP∆678-681-shTACC3

   Plasmid

   #69115

   Purpose

   Dual-promoter plasmid to express knockdown-proof human TACC3 (amino acids 678-681 deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.

   Depositor

   Stephen Royle

   Article

   Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

   Inserts

   Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
   shTACC3
   

   Use

   RNAi

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deletion of 678-681aa region. Silent mutations i…

   Promoter

   CMV

   Available Since

   Oct. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-U6/TO-shEsr1-CMV-TetR-eGFP

   Plasmid

   #233298

   Purpose

   DOX-inducible U6 driven shRNA against mouse Esr1

   Depositor

   Dayu Lin

   Article

   Golden et al bioRxiv [Preprint]. 2024 Sep 25:2023.12.

   Insert

   Esr1 shRNA (Esr1 Mouse)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. IronFistMUT.

   Plasmid

   #243008

   Purpose

   Iron binding deficient IronFist

   Depositor

   Roland Malli

   Article

   Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d

   Inserts

   hemerythrin-like-mNeonGreen (FBXL5 Human)
   T2A
   mCherry
   

   Use

   Gateway

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   H15A, H57A, E58A, E61A, H126A, E130A

   Promoter

   human phosphoglycerate kinase (hPGK)

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. CL20_mEGFP_FGFR3_TACC3

   Plasmid

   #205821

   Purpose

   Express mEGFP-tagged fusion protein, FGFR3_TACC3 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   FGFR3_TACC3 (FGFR3, TACC3 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCSC-LHX8-IRES-GBX1

   Plasmid

   #182235

   Purpose

   To convert human skin fibroblasts into induced basal forebrain cholinergic neurons (hiBFCNs) in combination with ASCL1, Sox11, FGF2 and two small molecules, forskolin and LDN-193189

   Depositor

   Chun-Li Zhang

   Article

   Ma et al Mol Neurodegener. 2020 Oct 21;15(1):61.

   Insert

   LHX8-IRES-GBX1 (LHX8, GBX1 Human)
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDF-hLPYK

   Plasmid

   #220231

   Purpose

   Inducible expression of human liver pyruvate kinase

   Depositor

   Aron Fenton, Liskin Swint-Kruse

   Article

   Swint-Kruse et al Database (Oxford). 2023 May 3;2023:baad0

   Insert

   Human liver pyruvate kinase (PKLR Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLNCX2-NGN2-IRES-GFP-T2A-Sox11

   Plasmid

   #90213

   Purpose

   To convert human skin fibroblasts into induced cholinergic neurons (hiCN) in combination with FGF2 and two small molecules, forskolin and dorsomorphin.

   Depositor

   Chun-Li Zhang

   Article

   Liu et al Nat Commun. 2013;4:2183. doi: 10.1038/nc

   Insert

   NGN2-IRES-GFP-T2A-Sox11 (Sox11, NEUROG2 Mouse, Human)
   

   Use

   Retroviral

   Promoter

   CMV

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTU1-A-pdh_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid

    #107576

    Purpose

    B. megaterium DSM319 pdh promoter, GFP+ with malachite green mRNA aptamer in Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

    Depositor

    Paul Freemont

    Article

    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

    Insert

    GFP+
    

    Use

    Synthetic Biology

    Tags

    B. megaterium DSM319 xylA leader sequence (MTSSKI…

    Expression

    Bacterial

    Mutation

    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A

    Promoter

    pdh promoter Bacillus megaterium DSM319

    Available Since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Exp-Rosa-EF1a-tTA-mCh-Rs1-TetO(sh)-2

    Plasmid

    #24419

    Depositor

    Edward Hsiao

    Article

    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

    Insert

    RS1 (HTR4 Human)
    

    Use

    Mouse Targeting

    Tags

    FLAG and mCherry

    Mutation

    Human 5HT4B receptor with D100A mutation, generat…

    Available Since

    March 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBrain-GFP-TACC3KDP∆682-688-shTACC3

    Plasmid

    #69116

    Purpose

    Dual-promoter plasmid to express knockdown-proof human TACC3 (682-688aa ch-TOG binding site deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.

    Depositor

    Stephen Royle

    Article

    Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

    Inserts

    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    shTACC3
    

    Use

    RNAi

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 682-688 (region containin…

    Promoter

    CMV

    Available Since

    Oct. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTU1-A-fbp_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid

    #107578

    Purpose

    B. megaterium DSM319 fbp promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

    Depositor

    Paul Freemont

    Article

    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

    Insert

    GFP+
    

    Use

    Synthetic Biology

    Tags

    B. megaterium DSM319 xylA leader sequence (MTSSKI…

    Expression

    Bacterial

    Mutation

    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A

    Promoter

    fbp promoter Bacillus megaterium DSM319

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCSC-NGN2-IRES-GFP-T2A-Sox11

    Plasmid

    #90214

    Purpose

    To convert human skin fibroblasts into induced motor neurons (hiMN) in combination with ISL1, LHX3, FGF2 and two small molecules, forskolin and dorsomorphin.

    Depositor

    Chun-Li Zhang

    Article

    Liu et al Cell Rep. 2016 Jan 5;14(1):115-28. doi:

    Insert

    NGN2-IRES-GFP-T2A-Sox11 (NEUROG2, Sox11 Mouse, Human)
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCSC-ISL1-T2A-LHX3

    Plasmid

    #90215

    Purpose

    To convert human skin fibroblasts into induced motor neurons (hiMN) in combination with NGN2, Sox11, FGF2 and two small molecules, forskolin and dorsomorphin.

    Depositor

    Chun-Li Zhang

    Article

    Liu et al Cell Rep. 2016 Jan 5;14(1):115-28. doi:

    Insert

    ISL1-T2A-LHX3 (ISL1, LHX3 Human)
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223-TRIB3

    Plasmid

    #23677

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    TRIB3 (TRIB3 Human)
    

    Use

    Gateway donor vector

    Mutation

    P5T

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pTU1-A-fba_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid

    #107582

    Purpose

    B. megaterium DSM319 fba promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

    Depositor

    Paul Freemont

    Article

    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

    Insert

    GFP+
    

    Use

    Synthetic Biology; E. coli and bacillus shuttle v…

    Tags

    B. megaterium DSM319 xylA leader sequence (MTSSKI…

    Expression

    Bacterial

    Mutation

    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A

    Promoter

    fba promoter Bacillus megaterium DSM319

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTU1-A-gap_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid

    #107577

    Purpose

    B. megaterium DSM319 gap promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

    Depositor

    Paul Freemont

    Article

    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

    Insert

    GFP+
    

    Use

    Synthetic Biology; E. coli and bacillus shuttle v…

    Tags

    B. megaterium DSM319 xylA leader sequence (MTSSKI…

    Expression

    Bacterial

    Mutation

    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A

    Promoter

    gapdh promoter - B. megaterium DSM319

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEMS1499

    Plasmid

    #29247

    Purpose

    Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple28 (CCL27 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Intron-LacZ-NLS

    Available Since

    Nov. 7, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pEMS1498

    Plasmid

    #29246

    Purpose

    Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple27 (CCL27 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Intron-LacZ-NLS

    Available Since

    Nov. 7, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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