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pRG/III-hs-MBP-A2aTr316-H10

Plasmid

#121683

Purpose

Bacterial expression of adenosine A2a receptor fusion protein

Depositor

Reinhard Grisshammer, Richard Henderson

Publication

Weiss et al Eur J Biochem. 2002 Jan;269(1):82-92.

Insert

adenosine A2a receptor (ADORA2A Human)

Tags

His10

Expression

Bacterial

Promoter

lac

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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hSUMO1-HIS

Plasmid

#118361

Purpose

This plasmid expresses the full length human SUMO1.

Depositor

Lea Sistonen

Publication

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hSUMO1 (SUMO1 Human)

Tags

HIS

Expression

Mammalian

Mutation

Please see Depositor Comments

Available since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLKO.1-shMBNL1-3964

Plasmid

#115459

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Publication

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shMBNL1-3964 (MBNL1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pROS14

Plasmid

#107928

Purpose

LEU2 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Publication

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pZLCv2-gMYOD1-3xFLAG-dCas9-HA-2xNLS

Plasmid

#106355

Purpose

CRISPR/Cas9 engineered chromatin immunoprecipitation of MYOD1 locus

Depositor

Stephen Tapscott

Publication

Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

Insert

MYOD1 gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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TUBG1_pLX307

Plasmid

#98376

Purpose

Lentiviral expression of TUBG1

Depositor

William Hahn, Sefi Rosenbluh

Publication

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

TUBG1 (TUBG1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PAK4 gRNA (BRDN0001147629)

Plasmid

#77573

Purpose

3rd generation lentiviral gRNA plasmid targeting human PAK4

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PAK4 (PAK4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pRSET-spa-GCaMP6f

Plasmid

#67563

Purpose

bacterial expression of a photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Publication

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6f

Tags

HIS-tag

Expression

Bacterial

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Available since

July 29, 2015

Availability

Academic Institutions and Nonprofits only
pQStrep2-PREI3

Plasmid

#31592

Depositor

Konrad Buessow

Publication

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

preimplantation protein 3 (MOB4 Human)

Tags

His and StrepII-tag

Expression

Bacterial

Available since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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M47 MBP-TEV-Chibby

Plasmid

#17195

Depositor

Randall Moon

Publication

Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

Insert

Chibby (CBY1 Human)

Tags

MBP

Expression

Bacterial

Available since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only
Hox A13b

Plasmid

#36468

Depositor

Robert Ho

Publication

Ahn et al Dev Biol. 2008 Oct 1;322(1):220-33. Epub

Insert

hoxA13b (hoxa11b Zebrafish)

Use

Riboprobe synthesis

Mutation

F232C

Promoter

T7

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
pSUPERIOR.puro-shREV3-4

Plasmid

#38029

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Publication

Knobel et al Neoplasia. 2011 Oct;13(10):961-70.

Insert

shREV3-4 (REV3L Human, Mouse, Rat, human)

Use

RNAi

Available since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
dopamine beta hydroxylase (dbh)_R (OZ526)

Plasmid

#27201

Depositor

Keith Joung

Publication

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting dbh (dbh Zebrafish)

Use

Zebrafish targeting

Available since

Feb. 8, 2011

Availability

Academic Institutions and Nonprofits only

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RCAS (A) cTbx3 del2 (CT#645)

Plasmid

#13921

Depositor

Cliff Tabin

Publication

Logan et al Development. 1998 Aug . 125(15):2825-35.

Insert

Tbx3 del (TBX3 Chicken)

Use

Retroviral; Avian expression

Mutation

Truncated Tbx3. Putative dominant negative.

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
piHGpd/p82

Plasmid

#1105

Depositor

Susan Lindquist

Publication

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

wild-type HSP82 regulated by the strong constitut…

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pAIP4_3RZY

Plasmid

#234156

Purpose

Heterologous protein expression of fatty acid-binding protein, adipocyte (ALBP) in Escherichia coli

Depositor

Christopher Bahl

Publication

Cárcamo Noriega et al Protein Sci. 2026 Sep;35(9):e70746. doi:

Insert

3RZY (FABP4 Human)

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCI5-CasRx-NES-HA (pKW387)

Plasmid

#229048

Purpose

Mammalian expression of CasRx-NES-HA

Depositor

S Chul Kwon

Publication

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CasRx (RfxCas13d)

Use

CRISPR

Tags

NES-HA

Expression

Mammalian

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pDL691

Plasmid

#231166

Purpose

T-DNA encoding TRV2 with ipt and mobile gRNAs targeting SlUGT75C1 and SlHAIRLESS

Depositor

Daniel Voytas

Publication

Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

Insert

ipt and mobile gRNAs targeting SlUGT75C1 and SlHAIRLESS

Expression

Plant

Promoter

PEBV sub-genomic

Available since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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hzHDAC10

Plasmid

#225542

Purpose

Expresses histone deacetylase 10 (HDAC10) humanized catalytic domain, also known as hzHDAC10, in Escherichia coli cells.

Depositor

David Christianson

Publication

Herbst-Gervasoni et al ACS Chem Biol. 2020 Aug 21;15(8):2154-21

Insert

Histone Deacetylase 10 humanized catalytic domain (hdac10 Zebrafish)

Tags

His6-MBP tag with cleavable TEV site

Expression

Bacterial

Mutation

Mutated A24E and D94A

Promoter

T7

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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gWiz_BM40-vGAT-VH-hIgG1-CH1-TS-HIS

Plasmid

#218436

Purpose

mammalian expression plasmid for the heavy chain fragment of an anti-vGAT Fab domain

Depositor

Axel Brunger

Publication

Gopal et al Curr Res Physiol. 2024 Feb 23;7:100121.

Insert

vGAT-Vh-hIgG1-CH1

Use

Affinity Reagent/ Antibody

Tags

BM40 and TwinStrep-6xHis

Expression

Mammalian

Promoter

CMV

Available since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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