Skip to main content
Addgene
Showing: 741 - 760 of 8435 results

  1. pCAG dCas9-KRAB-2A-EGFP

    Plasmid
    #92396
    Purpose
    CAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.
    Depositor
    Tatjana Sauka-Spengler
    Article
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Insert
    dCas9_KRAB_2A_EGFP
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    92396-plasmid-map-sequence-id-178234

    Enlarge

  11. Lenti-EF1alpha-dCas9-KRAB_Hygro

    Plasmid
    #192663
    Purpose
    3rd generation lenti vector encoding dCas9-KRAB with 2A Hygro resistance marker
    Depositor
    Jeffrey Miner
    Insert
    dCas9-KRAB
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A, D839A, H840A and N863A in Cas9
    Promoter
    EF1a
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192663-plasmid-map-sequence-id-379915

    Enlarge

  13. pInducer10b-EGFP-KRAS G12V

    Plasmid
    #164925
    Purpose
    Expresses EGFP cDNA and KRAS G12V cDNA in mammalian cells
    Depositor
    Ji Luo
    Article
    Carver et al PLoS One. 2014 Aug 5;9(8):e103836. doi:
    Inserts
    Enhanced green fluorescent protein
    KRAS proto-oncogene, GTPase (KRAS) G12V mutant (KRAS Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Glycine 12 changed to valine (G->T 3156)
    Promoter
    Ubc Promoter and Ubc promoter
    Available since
    May 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    164925-plasmid-map-sequence-id-333648

    Enlarge

  14. pLVCT-rtTR-KRAB-2SM2

    Plasmid
    #11779
    Purpose
    Tet-regulated (Tet-off) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation
    Depositor
    Didier Trono, Patrick Aebischer
    Article
    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:
    Insert
    CAG promoters, GFP, rtTR-KRAB-2SM2, Tet-off
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 17, 2006
    Availability
    Academic Institutions and Nonprofits only
    11779-plasmid-map-sequence-id-255304

    Enlarge

  16. pLVPT-rtTR-KRAB-2SM2

    Plasmid
    #11652
    Purpose
    Tet-regulated (Tet-off) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation
    Depositor
    Didier Trono, Patrick Aebischer
    Article
    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:
    Insert
    mPGK, GFP, rtTR-KRAB-2SM2, Tet-off
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 17, 2006
    Availability
    Academic Institutions and Nonprofits only
    11652-plasmid-map-sequence-id-6998

    Enlarge

  17. pUbi_dCas9-BFP-KRAB-gRNA

    Plasmid
    #221503
    Purpose
    CRISPRi machinery fish codon optimized expressing plasmid
    Depositor
    Alessandro Ori
    Insert
    dCas9-tagBFP-KRAB
    Use
    Fish expression
    Tags
    Expression
    Mutation
    D10A and H839A point mutations
    Promoter
    Zebrafish Ubiquitin promoter
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221503-plasmid-map-sequence-id-439415

    Enlarge

  19. pcDNA3-DrFLINC-AKAR-Kras

    Plasmid
    #182334
    Purpose
    Genetically encoded fluorescent biosensor for super-resolution imaging of PKA activity; targeted to the plasma membrane. Compatible with multiplexed super-resolution imaging using a Dronpa-tagged POI.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    DrFLINC-AKAR
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    182334-plasmid-map-sequence-id-363289

    Enlarge

Showing: 741 - 760 of 8435 results