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Addgene

We narrowed to 2,424 results for: MEA;

Showing: 741 - 760 of 2424 results
  1. pNB697

    Plasmid
    #60146
    Purpose
    Disintegrator plasmid encoding MET17 promoter driven red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).
    Depositor
    Insert
    RdLuc
    Use
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Expression
    Yeast
    Mutation
    Promoter
    MET17pr
    Available since
    Dec. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
  2. pNB788

    Plasmid
    #60165
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by RNR1 promoter.
    Depositor
    Insert
    FLuc-yEVenus
    Use
    Tags
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    RNR1pr
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
  3. pHS1625

    Plasmid
    #239834
    Purpose
    OrufIscB-REC E. coli expression for recombinant purification
    Depositor
    Insert
    OrufIscB-REC
    Use
    Tags
    14xHis-TwinStrep-bdSUMO
    Expression
    Bacterial
    Mutation
    Insertion of REC domain from NbaCas9-1
    Promoter
    T7
    Available since
    July 22, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. pHS1742

    Plasmid
    #239838
    Purpose
    OrufIscB-KRK mammalian expression
    Depositor
    Insert
    OrufIscB-KRK
    Use
    Tags
    Nucleoplasmin NLS-3xHA and SV40 NLS
    Expression
    Mammalian
    Mutation
    Insertion of REC domain from NbaCas9-1 + E137K/E4…
    Promoter
    CMV
    Available since
    July 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  5. pHS2096

    Plasmid
    #239835
    Purpose
    NovaIscB E. coli expression for recombinant purification
    Depositor
    Insert
    NovaIscB
    Use
    Tags
    14xHis-TwinStrep-bdSUMO
    Expression
    Bacterial
    Mutation
    Insertion of REC domain from NbaCas9-1 and swap o…
    Promoter
    T7
    Available since
    July 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  6. pA-CBh.TP-CNOT7-V5H6.MCh.Puro

    Plasmid
    #209946
    Purpose
    In mammalian cells expresses TP fused to a CNOT7 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.
    Depositor
    Inserts
    CCR4-NOT transcription complex subunit 7 (CNOT7 Human)
    mCherry fluorescent protein fused to puromycin resistance marker
    Use
    Tags
    TP (MS2 coat protein) and V5 epitope with H6 tag
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and CMV
    Available since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
  7. pA-CBh.TP-CNOT8-V5H6.MCh.Puro

    Plasmid
    #209947
    Purpose
    In mammalian cells expresses TP fused to a CNOT8 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.
    Depositor
    Inserts
    CCR4-NOT transcription complex subunit 8 (CNOT8 Human)
    mCherry fluorescent protein fused to puromycin resistance marker
    Use
    Tags
    TP (MS2 coat protein) and V5 epitope with H6 tag
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and CMV
    Available since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
  8. pA-CBh.TP-(inactive)CNOT6-V5H6.MCh.Puro

    Plasmid
    #209943
    Purpose
    In mammalian cells expresses TP fused to an inactive CNOT6 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.
    Depositor
    Inserts
    CCR4-NOT transcription complex subunit 6 (enzymatically inactive) (CNOT6 Human)
    mCherry fluorescent protein fused to puromycin resistance marker
    Use
    Tags
    TP (MS2 coat protein) and V5 epitope with H6 tag
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and CMV
    Available since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
  9. pLVUT-spC3GFP

    Plasmid
    #225487
    Purpose
    Tet-on lentiviral vector for controlled expression of a secretable and permeable form of C. botulinum exoenzyme C3 transferase fused with EGFP
    Depositor
    Insert
    Secretable and permeable exoenzyme C3 transferase
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Amino acids 30-251
    Promoter
    Human ubiquitin C
    Available since
    Sept. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 741 - 760 of 2424 results