We narrowed to 20,158 results for: Nov
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Plasmid#102931PurposeLentiviral expression of a lysosomal tag (LAMP1-mRFP1-Flag)DepositorInsertLAMP1
UseLentiviralTagsmRFP-2xFlagPromoterubcAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV FLEX Synaptophysin GFP
Plasmid#137188PurposeCre-dependent expression of synaptophysin-GFPDepositorInsertSynaptophysin GFP (Syp Mouse)
UseAAVAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GABA(A) receptor subunit B3SE
Plasmid#49171PurposepHluorin-tagged GABA A receptor subunit (beta 3) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surfaceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGABA(A) receptor subunit beta 3 (Gabrb3 Mouse)
Tagsmyc, pHGFP (pHluorin), and thrombin cleavage site…ExpressionMammalianMutationGTG (V) to TGC (C)PromoterCMVAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-EFS-PCP-GFPnls
Plasmid#121938PurposeCRISPR-Sirius plasmidDepositorInsertPCP-GFPnls
UseLentiviralTagssfGFPExpressionMammalianPromoterEFSAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CNC3G (CASANOVA-C3 with glycine linkers)
Plasmid#137192PurposeExpresses CNC3G (CASANOVA-C3 with glycine linkers), a blue light dependent NmeCas9 inhibitory proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCNC3G (CASANOVA-C3 with glycine linkers)
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHUE411
Plasmid#62203PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Hyg resistanceDepositorInsertsgRNA scaffold
zCas9
UsePlant binary vectorTags3x FLAG and NLSExpressionPlantMutationmaize codon optimizedPromoterOsU3 and UbiAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQCXIH-Flag-YAP-S127A
Plasmid#33092DepositorInsertYAP (YAP1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationS127APromoterCMV/MSVAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJK02
Plasmid#105133PurposeClostridium difficile CRISPR-cas9 mutagenesis plasmid traJ oriTDepositorInsertsUpstream & Downstream pyrE deletion region
tetR
Cas9
gRNA
UseE. coli - c. difficile shuttle vectorAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-PDZD8HA
Plasmid#105005PurposeMammalian Expression of PDZD8DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPDZD8 (Pdzd8 Mouse)
TagsHAExpressionMammalianPromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-K-I-N-CAAX
Plasmid#41640PurposeExpresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to wild-type Dronpa145K at C terminus and Dronpa145N at N terminus, for optogenetic activationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDronpa145K-IntersectinDH-Dronpa145N-CAAX
TagsCAAX boxExpressionMammalianMutationK145N on DronpaPromoterCMVAvailable sinceDec. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-PYL1-sfGFP-Emerin
Plasmid#121532Purposeexpression of PYL-SfGFP-Emerin, lentivirus vectorDepositorAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TO-dCas9-P2A-HSA
Plasmid#121936PurposeCRISPR-Sirius plasmidDepositorInsertdCas9-P2A-HSA
UseCRISPR and LentiviralTagsHSAExpressionMammalianPromoterCMV-TOAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SFG.wtCNa_opt.IRES.eGFP
Plasmid#22489DepositorInsertcodon optimised wild type Calcineurin A (PPP3CA Human)
UseRetroviralMutationCodon Optimised wtCNaAvailable sinceDec. 16, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-ABE6.3
Plasmid#102916Purposeexpresses ABE6.3 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertABE6.3
TagsNLSExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Synapsin-pHoenix-WPRE
Plasmid#70111Purposelentiviral expression of pHoenix (Synaptophysin-pHluorin-Arch3-mKate2) driven by human Synapsin I promoter for optogenetic acidification of synaptic vesiclesDepositorInsertpHoenix (Syp Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Rat)
UseLentiviralTagsArch3, H+/K+ ATPase beta-subunit, mKate2, and pHl…PromoterSynapsinAvailable sinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAJM.773
Plasmid#108527PurposeVanRAM + PVanCC-YFP Reporter. Van sensor for use in non-Marionette strain.DepositorInsertVanRAM + PVanCC-YFP
ExpressionBacterialAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-PIPK1 gamma 87
Plasmid#22300DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman phosphatidylinositol phosphate kinase type 1 gamma 87 (PIP5K1C Human)
TagsGFPExpressionBacterial and MammalianAvailable sinceDec. 10, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMSCV-Flag-cMyc T58A
Plasmid#20075DepositorAvailable sinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmScarlet-i_alphaTubulin_C1
Plasmid#85047PurposeIn vivo visualization of microtubules (can be used for colocalization studies)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAlpha-Tubulin (TUBA1B Human)
TagsmScarlet-iExpressionMammalianPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
M22: pHR-SFFVp-NLS-iLID::EGFP::FTH1
Plasmid#122147PurposeSelf-assebled 24-mer tagged with NLS, iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.DepositorAvailable sinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by