We narrowed to 1,231 results for: POLI

1. pET21a-CampR

   Plasmid

   #51162

   Depositor

   Christopher Voigt

   Article

   Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

   Insert

   CampR
   

   Use

   Synthetic Biology

   Tags

   6x-his

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCellFree_G03 PC

   Plasmid

   #67132

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   PC (PC Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pJB75

   Plasmid

   #226320

   Purpose

   E. coli protein expression for protein purification

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   6xHis-(Y to A MR1-6)-strep
   

   Tags

   6xHis and Strep

   Expression

   Bacterial

   Mutation

   N-terminal domain deleted, C-terminal domain dele…

   Promoter

   T7

   Available Since

   Oct. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFH77

   Plasmid

   #125816

   Purpose

   Level0 enCas9-PolI3M-TBD, wheat codon optimized

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   enCas9-PolI3M-TBD, wheat codon optimized
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. H2B-mNeonGreen-IRESpuro2

   Plasmid

   #183745

   Purpose

   Expresses H2B-mNeonGreen

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   H2B (H2BC16P Human)
   

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Ki67-mNeonGreen-IRESpuro2

   Plasmid

   #183737

   Purpose

   Expresses Ki67-mNeonGreen

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   MKI67 (MKI67 Human)
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBP-T7RNAP

   Plasmid

   #74096

   Purpose

   T7 RNA polymerase PCR cloned from BL21 (DE3) genomic DNA

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   T7 RNAP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   None

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET21a - TR

   Plasmid

   #89621

   Purpose

   [Edit] Expresses recombinant 6HIS tagged T. cruzi trypanothione reductase protein in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   Thioredoxin
   

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Available Since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pgRNA

   Plasmid

   #215560

   Purpose

   Constitutively expressed sgRNA targeting a desired gene (this case pta), ColE1 origin, KanR

   Depositor

   Brian Pfleger

   Article

   Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

   Insert

   gRNA
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. hMTP-flag

    Plasmid

    #138335

    Purpose

    Expresses MTP

    Depositor

    Mahmood Hussain

    Article

    Rava et al J Biol Chem. 2006 Apr 21;281(16):11019-2

    Insert

    MTTP (MTTP Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET21a - NR

    Plasmid

    #89625

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi nitrate reductase in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Nitrate reductase
    

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBP-lldR

    Plasmid

    #74095

    Purpose

    L-lactate repressor (LldR) from E. coli MG1655

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    lldR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    None

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCellFree_G03 TFEC

    Plasmid

    #67060

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TFEC (TFEC Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pTU1-A-RFP

    Plasmid

    #72939

    Purpose

    Level 1 Destination vector - Position A

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 1 - A site
    

    Use

    Synthetic Biology

    Mutation

    Beta-lactamase - G717C (nucleotide) - silent muta…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCellFree_G03 TCF12

    Plasmid

    #67113

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TCF12 (TCF12 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pMSP2

    Plasmid

    #26282

    Depositor

    Stephen Sligar

    Article

    Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

    Insert

    MSP2
    

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    synthetic gene of dimer of deletion mutant (1-43)…

    Available Since

    April 7, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pBP-SJM901

    Plasmid

    #72966

    Purpose

    Level 0 - SJM901 promoter (5' CTAT/3' GTAC fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    SJM901 promoter
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SiT-EGFP

    Plasmid

    #166943

    Purpose

    mammalian expression of N' Sialyl Transferase tagged with EGFP

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Patterson et al Cell. 2008 Jun 13;133(6):1055-67. doi: 1

    Insert

    Sialyl Transferase N-terminus
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCellFree_G03 CYP2A13

    Plasmid

    #67103

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CYP2A13 (CYP2A13 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pCellFree_G03 WWP1

    Plasmid

    #67125

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    WWP1 (WWP1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge