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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,041 results for: biotin

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Showing: 741 - 760 of 1041 results

  2. JB96

    Plasmid
    #128923
    Purpose
    mammalian expression, proximity labelling, mitochondrial relocalisation
    Depositor
    Sean Munro
    Publication
    Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nRasSN-BirA*-HA-MAO (NRAS Human)
    Expression
    Mammalian
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    128923-plasmid-map-sequence-id-254214

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  3. MT4

    Plasmid
    #223332
    Purpose
    Mapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells
    Depositor
    Xiaohong Zhu
    Publication
    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1
    Insert
    3HA-miniTurboID-EGFP-OMP25 (Synj2bp Rat)
    Tags
    3HA-miniTurboID-EGFP
    Expression
    Plant
    Promoter
    CaMV35S
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    223332-plasmid-map-sequence-id-455589

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  5. ENDOD1-bio-His

    Plasmid
    #53155
    Purpose
    Expresses full-length ENDOD1 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    ENDOD1 (ENDOD1 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53155-plasmid-map-sequence-id-82782

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  7. p663-UBC-miniTurbo-V5-PIP5K1A_IDG-K

    Plasmid
    #214462
    Purpose
    Gateway destination clone of PIP5K1A (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-PIP5K1A (PIP5K1A Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available since
    March 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    214462-plasmid-map-sequence-id-425204

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  9. piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1628)

    Plasmid
    #209086
    Purpose
    Ngn2 mediated cortical neuron induction, SNCA(Avi tagged) expression, Synapsin promoter BirA ligase expression
    Depositor
    Vikram Khurana
    Publication
    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    BirA
    Tags
    AviTag
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    TRE4G and hSyn
    Available since
    Dec. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    209086-plasmid-map-sequence-id-417159

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  11. XX01CAPN1A-c001

    Plasmid
    #175491
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-A714 (CAPN1); EF hand 1-5, H86-S268 (CAPNS1). CAPN1 can be biotinylated. Codon optimised.
    Depositor
    Opher Gileadi
    Insert
    CAPN1 / CAPNS1 (CAPN1 Human)
    Tags
    AviTag and His6-TEV
    Expression
    Bacterial
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175491-plasmid-map-sequence-id-353165

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  12. XX01CAPN2A-c001

    Plasmid
    #175492
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-L700 (CAPN2); EF hand 1-5, H86-S268 (CAPNS1). CAPN2 can be biotinylated. Codon optimised.
    Depositor
    Opher Gileadi
    Insert
    CAPN2 / CAPNS1 (CAPN2 Human)
    Tags
    AviTag and His6-TEV
    Expression
    Bacterial
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175492-plasmid-map-sequence-id-353163

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  13. pET29b-AviTag-eGFP-dspB(E184Q)-6xHis

    Plasmid
    #175784
    Purpose
    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.
    Depositor
    Mark Nitz
    Publication
    Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12
    Insert
    Dispersin B
    Tags
    AviTag, Hexahistidine tag, and eGFP
    Expression
    Bacterial
    Mutation
    E184Q
    Promoter
    T7
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    175784-plasmid-map-sequence-id-345284

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  14. pET29b-AviTag-eGFP-dspB(E184Q W330Y)-6xHis

    Plasmid
    #175801
    Purpose
    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.
    Depositor
    Mark Nitz
    Publication
    Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12
    Insert
    Dispersin B
    Tags
    AviTag, Hexahistidine tag, and eGFP
    Expression
    Bacterial
    Mutation
    E184Q W330Y
    Promoter
    T7
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    175801-plasmid-map-sequence-id-345278

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  18. p663-UBC-miniTurbo-V5-CSNK1G2_IDG-K

    Plasmid
    #214459
    Purpose
    Gateway destination clone of CSNK1G2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CSNK1G2 (CSNK1G2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214459-plasmid-map-sequence-id-423212

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  19. D92_Ld_360170

    Plasmid
    #135905
    Purpose
    Expression of Leishmania donovani ectodomain in mammalian cells
    Depositor
    Gavin Wright
    Publication
    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hypothetical protein, conserved
    Tags
    biotinylation sequence and His tag
    Expression
    Mammalian
    Available since
    March 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    135905-plasmid-map-sequence-id-274668

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  20. D44_Ld_180090

    Plasmid
    #135858
    Purpose
    Expression of Leishmania donovani ectodomain in mammalian cells
    Depositor
    Gavin Wright
    Publication
    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    alpha glucosidase II subunit, putative
    Tags
    biotinylation sequence and His tag
    Expression
    Mammalian
    Available since
    March 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    135858-plasmid-map-sequence-id-274512

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Showing: 741 - 760 of 1041 results