We narrowed to 18,457 results for: multi

1. pAAV-Syn-VARNAM2-Kv2.1PR

   Plasmid

   #195524

   Purpose

   Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted

   Depositor

   Vincent Pieribone

   Article

   Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

   Insert

   VARNAM2-Kv2.1 proximal restriction sequence
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   VARNAM SI linker

   Promoter

   Synapsin

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV5.1-SARS-CoV-2 Nsp3

   Plasmid

   #204205

   Purpose

   Mammalian expression of SARS-CoV-2 Nsp3

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )
   

   Tags

   FLAGx3

   Expression

   Mammalian

   Mutation

   none, from obtained clone

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX SH3(2)-4R

   Plasmid

   #112090

   Purpose

   Bacterial expression plasmid containing a GST tag with 4 repeats of the second SH3 domain from human NCK1.

   Depositor

   Michael Rosen

   Article

   Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

   Insert

   SH3(2)-4R (NCK1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   Lac

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYLsgRNA-OsU6a/LacZ

   Plasmid

   #66195

   Purpose

   cloning of sgRNAs for expression in plants

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Type

   Empty backbone

   Use

   Cloning vector

   Promoter

   OsU6a

   Available Since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYLsgRNA-OsU3m

   Plasmid

   #66193

   Purpose

   cloning of sgRNAs for expression in plants

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Type

   Empty backbone

   Use

   Cloning vector

   Promoter

   OsU3m (SpeI site destroyed)

   Available Since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. NPY1R-DuET

   Plasmid

   #213354

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   NPY1R (NPY1R Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA5/FRT/TO_TOMM20_HaloTag9

   Plasmid

   #169335

   Purpose

   Expression of HaloTag9 at the mitochondrial surface of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   TOMM20-HaloTag9 (TOMM20 Human)
   

   Tags

   HaloTag9

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEntry_SARS-CoV-1-Spike

   Plasmid

   #168864

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Pascal Falter-Braun

   Article

   Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

   Insert

   SARS-CoV-1-Spike (S )
   

   Use

   Gateway-compatible entry vector

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAC93-pmax-dCas9VP160

   Plasmid

   #48225

   Purpose

   dCas9VP160 on pmax expression vector

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A) fusion with VP160 activation domain
   

   Use

   CRISPR

   Tags

   HA Tag and VP160

   Expression

   Mammalian

   Mutation

   D10A;H840A (catalytically inactive)

   Promoter

   CAGGS

   Available Since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR-JNKKTRmRuby2

    Plasmid

    #59148

    Purpose

    ORF encoding JNK KTR mRuby2 flanked by Gateway sequences

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    JNK Kinase Translocation Reporter (MAPK8 Human, Mouse)
    

    Tags

    mRuby2

    Available Since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX SH3(2)-3R

    Plasmid

    #112091

    Purpose

    Bacterial expression plasmid containing a GST tag with 3 repeats of the second SH3 domain from human NCK1.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    SH3(2)-3R (NCK1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    Lac

    Available Since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-RAB-EKARev-NLS

    Plasmid

    #118476

    Purpose

    ddRFP-based single-color ERK biosensor targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    RAB-EKARev-NLS
    

    Tags

    6xHIS, Nuclear localization signal (NLS), T7 tag …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMuLE ENTR CMV Renilla Luciferase L5-L2

    Plasmid

    #62186

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and renilla luciferase module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    Renilla luciferase
    

    Use

    Luciferase; Mule gateway entry vector

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. ECFP-Bcr

    Plasmid

    #36415

    Depositor

    John Groffen, Nora Heisterkamp

    Article

    Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

    Insert

    breakpoint cluster region (BCR Human)
    

    Tags

    ECFP

    Expression

    Mammalian

    Available Since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

15. SSTR3-DuET

    Plasmid

    #213378

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    SSTR3 (SSTR3 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. talin1-head

    Plasmid

    #182996

    Purpose

    expression of talin1 head wt in mammalian cells

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

    Insert

    talin1 head (Tln1 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. OPN3-DuET

    Plasmid

    #213357

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    OPN3 (OPN3 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEntry_HCoV-OC43-nsp12

    Plasmid

    #168949

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    HCoV-OC43-nsp12 (ORF1ab )
    

    Use

    Gateway-compatible entry vector

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-sgRb1/Cre

    Plasmid

    #89647

    Purpose

    Expresses an Rb1-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

    Insert

    gRNA targeting Rb1 (Rb1 Mouse)
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CaMKII-Ace-mNeon2

    Plasmid

    #195526

    Purpose

    Green fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    Ace-mNeon2
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    Ace-mNeon 81S; SY linker

    Promoter

    CaMKII

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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