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pKLV2-U6gRNA5(Nelfcd-g1)-PGKpuroBFP-W

Plasmid

#105025

Purpose

Lentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Nelfcd (Th1l Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Nelfcd-g2)-PGKpuroBFP-W

Plasmid

#105026

Purpose

Lentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Nelfcd (Th1l Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shEsco2

Plasmid

#160957

Purpose

Esco2 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Esco2 shRNA (Esco2 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Mfsd10

Plasmid

#120238

Purpose

Expresses V5-tagged Mfsd10 in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Mfsd10 (Mfsd10 Mouse)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pHTSHP-Smt-IIShhN

Plasmid

#121135

Purpose

Expresses N-terminal SUMO tagged II-ShhN in bacteria

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Shh (Shh Mouse)

Tags

HIS, HRV3C site, MBP, and Smt tag

Expression

Bacterial

Mutation

Amino acid sequence from 26-195, with two extra i…

Promoter

tac

Available Since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pET41a-MED31

Plasmid

#15437

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED31 (Med31 Mouse)

Tags

GST and His

Expression

Bacterial

Mutation

N/A

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
myc-Med31 pBacPAK8

Plasmid

#15375

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

Med31 (Med31 Mouse)

Use

Baculovirus construction

Tags

myc

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pGEX4T-2 GST-mouse Fis1 (ΔTM domain)

Plasmid

#252268

Purpose

Expression of GST-mouse Fis1 (ΔTM domain) in bacteria

Depositor

David Chan

Article

Nakajima et al Curr Biol. 2026 Mar 9;36(5):1205-1219.e8

Insert

mouse Fis1 (deltaTM domain) (Fis1 Mouse)

Tags

GST

Expression

Bacterial

Mutation

moue Fis1 1-121 amino acids

Promoter

tac

Available Since

Feb. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pQCXIP EGFP-mouse Fis1 (L110A)

Plasmid

#252265

Purpose

Retroviral expression of EGFP-mouse Fis1 (L110A)

Depositor

David Chan

Article

Nakajima et al Curr Biol. 2026 Mar 9;36(5):1205-1219.e8

Insert

mouse Fis1 (L110A) (Fis1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Mutating leucine 110 to alanine (L110A) in Fis1 r…

Promoter

CMV

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

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pQCXIP EGFP-mouse Fis1 (W40A)

Plasmid

#252263

Purpose

Retroviral expression of EGFP-mouse Fis1 (W40A)

Depositor

David Chan

Article

Nakajima et al Curr Biol. 2026 Mar 9;36(5):1205-1219.e8

Insert

mouse Fis1 (W40A) (Fis1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Mutating tryptophan 40 to alanine (W40A) in Fis1 …

Promoter

CMV

Available Since

Feb. 24, 2026

Availability

Academic Institutions and Nonprofits only

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pQCXIP EGFP-mouse Fis1 (V79A)

Plasmid

#252264

Purpose

Retroviral expression of EGFP-mouse Fis1 (V79A)

Depositor

David Chan

Article

Nakajima et al Curr Biol. 2026 Mar 9;36(5):1205-1219.e8

Insert

mouse Fis1 (V79A) (Fis1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Mutating valine 79 to alanine (V79A) in Fis1 resu…

Promoter

CMV

Available Since

Feb. 24, 2026

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Zmynd8-exon minimal CMV pCDNA5

Plasmid

#212078

Purpose

LR vector for integration of Zmynd8-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Zmynd8-exon (Zmynd8 Mouse)

Expression

Mammalian

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pEE019

Plasmid

#176776

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

211

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE020

Plasmid

#176777

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

212

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE029

Plasmid

#176786

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

306

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE007

Plasmid

#176764

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

103

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shTgm5-1

Plasmid

#180394

Purpose

Producing AAV that encodes mouse Tgm5 shRNA-1 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shTgm5-1 (Tgm5 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shTgm5-2

Plasmid

#180395

Purpose

Producing AAV that encodes mouse Tgm5 shRNA-2 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shTgm5-2 (Tgm5 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shTgm5-3

Plasmid

#180396

Purpose

Producing AAV that encodes mouse Tgm5 shRNA-3 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shTgm5-3 (Tgm5 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shCdkn3

Plasmid

#160955

Purpose

Cdkn3 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Cdkn3 shRNA (Cdkn3 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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