We narrowed to 24,185 results for: promoter

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pLenti6.3delta4 GPR3-V5

Plasmid

#172601

Purpose

Lentiviral plasmid expressing c-terminal V5-tagged GPR3

Depositor

Robert Screaton

Article

Iorio et al Commun Biol. 2021 Jul 23;4(1):907. doi:

Insert

G protein-Coupled Receptor 3 (GPR3 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET-DEST42 -SSRP1

Plasmid

#170439

Purpose

Express drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.

Depositor

Joan Conaway

Article

Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

Insert

ssrp1 (Ssrp Fly)

Tags

6xHis and V5

Expression

Bacterial

Promoter

T7

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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human TLNRD1-4H pET151

Plasmid

#159386

Purpose

Expresses human TLNRD1 4-helix domain (residues 143-273) in bacterial cells

Depositor

Ben Goult

Article

Cowell et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

Talin Rod Domain Containing Protein 1 (TLNRD1 Human)

Tags

his-tag, TEV cleavage site

Expression

Bacterial

Mutation

TLNRD1 4-helix domain (residues 143-273)

Promoter

T7

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcnj13-M135R-EGFP

Plasmid

#164944

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish gain-of-function kcnj13 mutant, M135R

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnj13 (kcnj13 Zebrafish)

Use

Tol2 transposon destination vector

Tags

EGFP

Mutation

Stop codon deleted; M135R

Promoter

actinb (actb2) promoter

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-TAPBPR-tapasinCT deltaALAS

Plasmid

#153471

Purpose

Mammalian expression of FLAG-tagged TAPBPR with tapasin CT deltaALAS

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBPR (TAPBPL Human)

Tags

FLAG

Expression

Mammalian

Mutation

C terminal tail of tapasin, deletion of amino aci…

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-TAPBPR-tapasinCT delta24-36

Plasmid

#153472

Purpose

Mammalian expression of FLAG-tagged TAPBPR with tapasin CT delta24-36

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBPR (TAPBPL Human)

Tags

FLAG

Expression

Mammalian

Mutation

C terminal tail of tapasin, deletion of amino aci…

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pSico_U6-PLOD2 sgRNA3

Plasmid

#136458

Purpose

Expression of gRNA against human PLOD2

Depositor

Antonio Amelio

Article

Sato et al Neoplasia. 2021 Jun 6;23(6):594-606. doi

Insert

gRNA against human PLOD2 (PLOD2 Synthetic, Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pSico_U6-PLOD2 sgRNA5

Plasmid

#136460

Purpose

Expression of gRNA against human PLOD2

Depositor

Antonio Amelio

Article

Sato et al Neoplasia. 2021 Jun 6;23(6):594-606. doi

Insert

gRNA against human PLOD2 (PLOD2 Synthetic, Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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deltaVP1 + VP2C-GFP-MIOX

Plasmid

#166682

Purpose

Yeast integrative plasmid for expressing deltaVP1 (GAL1 promoter) and mouse myo-inositol oxygenase fused to VP2C-GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

Inserts

VP2C-GFP-MIOX
Murine polyomavirus deltaVP1 (NLS deletion mutant)

Use

Synthetic Biology

Expression

Yeast

Promoter

GAL1 and GAL10

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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wtVP1 + VP2C-GFPdeg

Plasmid

#166678

Purpose

Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1 (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

Inserts

VP2C-GFPdeg
Wild-type Murine polyomavirus VP1

Use

Synthetic Biology

Expression

Yeast

Promoter

GAL1 and GAL10

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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deltaVP1 + VP2C-GFPdeg

Plasmid

#166677

Purpose

Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

Inserts

VP2C-GFPdeg
Murine polyomavirus deltaVP1 (NLS deletion mutant)

Use

Synthetic Biology

Expression

Yeast

Promoter

GAL1 and GAL10

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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wtVP1 + VP2C-GFP

Plasmid

#166674

Purpose

Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

Inserts

VP2C-GFP
Wild-type Murine polyomavirus VP1

Use

Synthetic Biology

Expression

Yeast

Promoter

GAL1 and GAL10

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-Hs.KCNJ13-IRES-EGFP

Plasmid

#164945

Purpose

Tol2 construct: actinb (actb2) promoter driven human KCNJ13 and EGFP”. This will reflect the linkage of KCNJ13 and EGFP

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

KCNJ13 (KCNJ13 Human)

Use

Tol2 transposon destination vector

Tags

IRES-EGFP

Mutation

Stop codon deleted

Promoter

actinb (actb2) promoter

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcnj13-Q153H-EGFP

Plasmid

#164943

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish loss-of-functionkcnj13 mutant, Q153H and M154I

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnj13 (kcnj13 Zebrafish)

Use

Tol2 transposon destination vector

Tags

EGFP

Mutation

Stop codon deleted; Q153H; M154I

Promoter

actinb (actb2) promoter

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcnj13-T131A-EGFP

Plasmid

#164942

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish loss-of-function kcnj13 mutant, T131A and G133R

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnj13 (kcnj13 Zebrafish)

Use

Tol2 transposon destination vector

Tags

EGFP

Mutation

Stop codon deleted; T131A; G133R

Promoter

actinb (actb2) promoter

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcnj1b-IRES-EGFP

Plasmid

#164946

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj1b and EGFP

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnj1b (kcnj1b Zebrafish)

Use

Tol2 transposon destination vector

Tags

IRES-EGFP

Promoter

actinb (actb2) promoter

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6B CARD8 UPA-CARD-mCherry R495E

Plasmid

#164016

Purpose

Mammalian expression vector encoding CARD8 UPA-CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

mCherry

Expression

Mammalian

Mutation

R495E

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6B CARD8 UPA-CARD-mCherry Y527A

Plasmid

#164018

Purpose

Mammalian expression vector encoding CARD8 UPA-CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Y527A

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C

Plasmid

#164023

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C R459E

Plasmid

#164024

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, R459E

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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