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pPD113 VP16-ZF43(AAAA)

Plasmid

#138818

Purpose

Constitutive expression of VP16-ZF43(AAAA) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF43(AAAA)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R6A, R15A, R43A, R71A

Promoter

CMV

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPD112 VP16-ZF43(AAAR)

Plasmid

#138817

Purpose

Constitutive expression of VP16-ZF43(AAAR) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF43(AAAR)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R6A, R15A, R43A

Promoter

CMV

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPD111 VP16-ZF43(AARR)

Plasmid

#138816

Purpose

Constitutive expression of VP16-ZF43(AARR) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF43(AARR)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R6A, R15A

Promoter

CMV

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPD110 VP16-ZF43(ARRR)

Plasmid

#138815

Purpose

Constitutive expression of VP16-ZF43(ARRR) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF43(ARRR)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R6A

Promoter

CMV

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPD109 VP16-ZF43(RAAA)

Plasmid

#138814

Purpose

Constitutive expression of VP16-ZF43(RAAA) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF43(RAAA)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R15A, R43A, R71A

Promoter

CMV

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPD108 VP16-ZF43(RAAR)

Plasmid

#138813

Purpose

Constitutive expression of VP16-ZF43(RAAR) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF43(RAAR)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R15A, R43A

Promoter

CMV

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJM572 ZF6x6-C intC-ZF1-NLS-HA in TUPV2

Plasmid

#161559

Purpose

Inducible expression of intC-ZF1-NLS-HA under the ZF6x6-C promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

intC-ZF1-NLS-HA

Use

Synthetic Biology

Tags

HA

Expression

Mammalian

Promoter

ZF6x6-C

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJM589 EF1α intN-ZF10-NLS-HA in TUPV2

Plasmid

#161565

Purpose

Constitutive expression of intN-ZF10-NLS-HA under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

intN-ZF10-NLS-HA

Use

Synthetic Biology

Tags

HA

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJB004 CMV 3xFLAG-NLS-VP16-intN

Plasmid

#161549

Purpose

Constitutive expression of 3xFLAG-NLS-VP16-intN under the CMV promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-VP16-intN

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJM516 EF1α intC-ZF1-NLS-HA in TUPV2

Plasmid

#161551

Purpose

Constitutive expression of intC-ZF1-NLS-HA under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

intC-ZF1-NLS-HA

Use

Synthetic Biology

Tags

HA

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJM522 EF1α intC-ZF10-NLS-HA in TUPV2

Plasmid

#161552

Purpose

Constitutive expression of intC-ZF10-NLS-HA under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

intC-ZF10-NLS-HA

Use

Synthetic Biology

Tags

HA

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK234_058

Plasmid

#123984

Purpose

Encodes the spCas9 Safetargeting Chr11 gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sg Safetargeting Chr11

Expression

Mammalian

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK234_038

Plasmid

#123917

Purpose

Encodes the spCas9 pSV40 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA-pSV40

Expression

Mammalian

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK234_043

Plasmid

#123913

Purpose

Encodes the spCas9 pSV40 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA-pSV40

Expression

Mammalian

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIE059 ZF43x1 mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138740

Purpose

Integration Vector, assembled with pHIE043 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x1

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIE060 ZF43x3-C mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138741

Purpose

Integration Vector, assembled with pHIE044 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x3-Compact

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIE063 ZF43x3-S mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138744

Purpose

Integration Vector, assembled with pHIE047 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x3-Spaced

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIE064 ZF43x6-S mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138745

Purpose

Integration Vector, assembled with pHIE048 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Spaced

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIE062 ZF43x12-C mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138743

Purpose

Integration Vector, assembled with pHIE046 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Compact

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJM466 EF1a VP16-ZF43 (TUPV2)

Plasmid

#138730

Purpose

mMoClo TUPV, with EF1alpha promoter and VP16-ZFa in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF43

Use

Synthetic Biology

Expression

Mammalian

Promoter

hEF1alpha

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge