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pIntegrase_12

Plasmid

#60583

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_12

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pIntegrase_13

Plasmid

#60584

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_13

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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VEE-EGFP-IRES-PuroR

Plasmid

#242414

Purpose

Spectral unmixing: EGFP only

Depositor

Ewan St. John Smith

Article

Lim et al bioRxiv 2024.09.24.614636

Inserts

EGFP
IRES-PuroR

Use

Self-amplifying rna

Expression

Mammalian

Available Since

Sept. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pBZS-M.CviPI

Plasmid

#198355

Purpose

Express M.CviPI in bacterial cells.

Depositor

Takashi Ito

Article

Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

Insert

M.CviPI (M.CviPI )

Expression

Bacterial

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pHaMDRwt

Plasmid

#10957

Depositor

Michael Gottesman

Article

Pastan et al Proc Natl Acad Sci U S A. 1988 Jun . 85(

Insert

MDR1 (ABCB1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

March 14, 2006

Availability

Academic Institutions and Nonprofits only

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Inducible tdTomato

Plasmid

#183718

Purpose

tdTomato expression from dox-inducible promoter; plasmid encodes the TetR inducer

Depositor

Jeffrey Gerst

Article

Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

Inserts

tdTomato
rTetR

Use

Lentiviral

Promoter

TRE3GV and UbiC

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-AncMEcoGII291

Plasmid

#205024

Purpose

Expressing engineered M.EcoGII with higher thermostability and DNA methylation activity

Depositor

Paola Laurino

Article

Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

Insert

Ancestral M.EcoGII 291

Tags

His6x tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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TATA-GFP

Plasmid

#110833

Purpose

negative control

Depositor

Michael Lewis

Article

Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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Golgi-targeted optoPLD

Plasmid

#140058

Purpose

Encodes CRY2-mCherry-PLD-P2A-CIBN-Sialyltransferase(TM) for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Tags

CIBN, CRY2, P2A, Sialyltransferase(TM), and mCher…

Expression

Mammalian

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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HyPer7.2DAAO-CAV

Plasmid

#168303

Purpose

Chemogenetic fluorescent H2O2 reporter targeted to the caveolae

Depositor

Thomas Michel

Article

Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

Insert

HyPer7.2DAAO-CAV

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pYIC

Plasmid

#18673

Depositor

Han Htun

Article

Nie et al Nucleic Acids Res. 2006 . 34(19):5528-4

Type

Empty backbone

Tags

3Myc-EYFP-HA-His6 and ECFP-HA-His6

Expression

Mammalian

Available Since

Aug. 12, 2008

Availability

Academic Institutions and Nonprofits only

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pCAGGS-HA-hUBE1L

Plasmid

#12438

Depositor

Dong-Er Zhang

Article

Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

Insert

UBE1L (UBA7 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
SpCas9-CBE6b-IVT

Plasmid

#215834

Purpose

Template for in vitro transcription of CBE6b (with SpCas9)

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6b-SpCas9-2xUGI

Use

In vitro transcription template

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pMT-Bip-GFP:V5:KDEL

Plasmid

#69917

Purpose

An endoplasmic reticulum (ER) marker used to visulaize the ER structure in Drosophilia S2 cells.

Depositor

Kenneth Irvine

Article

Okajima et al Science. 2005 Mar 11;307(5715):1599-603.

Insert

GFP

Tags

Drosophila BiP signal sequence, KDEL ER targeting…

Expression

Insect

Promoter

pMT

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-hCDC6p-Venus-puro

Plasmid

#212666

Purpose

Express Venus under CDC6 promoter

Depositor

Yumi Konagaya, Tobias Meyer

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

hCDC6p-Venus

Use

Lentiviral

Promoter

hCDC6p

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pTK_Sox10_enh_84_Citrine

Plasmid

#130584

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox10_enh_84

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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PB-EF1a-TetOn3G

Plasmid

#104543

Purpose

PiggyBac vector encoding Tet-On 3G constitutively expressed by EF1a promoter

Depositor

David Vereide

Article

Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

Insert

Tet-On 3G

Expression

Bacterial and Mammalian

Promoter

EF1a

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry-TPX2

Plasmid

#31227

Depositor

Patricia Wadsworth

Article

Ma et al Mol Biol Cell. 2010 Mar 15. 21(6):979-88

Insert

TPX2 (TPX2 Human)

Tags

mCherry

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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hNedd4-2

Plasmid

#83433

Purpose

full length Nedd4L with His Xpress tag

Depositor

Christie Thomas

Article

Itani et al Am J Physiol Renal Physiol. 2005 Aug;289

Insert

human Nedd4L with C2 domain (NEDD4L Human)

Tags

His Tag (6x) and Xpress Epitope Tag

Expression

Mammalian

Promoter

CMV

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pALPS puro miR30-L1221

Plasmid

#101335

Purpose

negative control for knockdowns target site: CTTGTCGATGAGAGCGTTTGT

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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